Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,089,170 T→G V296G (GTC→GGC)  galP → D‑galactose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,089,1700TG98.4% 212.1 / NA 64V296G (GTC→GGC) galPD‑galactose transporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (26/36);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTA  >  NC_000913/3088941‑3089399
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     
gAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTggcggc                                                                                                                                                                                                                                   <  1:250903/234‑1 (MQ=255)
     gAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCa                                                                                                                                                                                                                            <  2:605694/236‑1 (MQ=255)
      aaaCGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTggcggc                                                                                                                                                                                                                                   <  1:67937/228‑1 (MQ=255)
                            gTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCa                                                                                                                                                                                                     >  1:388378/1‑236 (MQ=255)
                              gAAAGTTCGAAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATTAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGAAAt                                                                                                                                                                                                   <  1:715540/236‑1 (MQ=255)
                                     tGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCa                                                                                                                                                                                                                            >  1:150095/1‑204 (MQ=255)
                                     tGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCa                                                                                                                                                                                                                            <  2:150095/204‑1 (MQ=255)
                                                       gCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTg                                                                                                                                                                                 <  1:26540/229‑1 (MQ=255)
                                                       gCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTg                                                                                                                                                                                 <  1:527299/229‑1 (MQ=255)
                                                             agcTGTTTAAAGAGAACAGCAACTTCCGACGCGCGGTGTGCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATTTATTACGCGACGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAcc                                                                                                                                                                     <  2:61086/234‑1 (MQ=255)
                                                                      aaGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaacg                                                                                                                                                           <  1:493467/236‑1 (MQ=255)
                                                                        gagaACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaacgct                                                                                                                                                         <  1:405469/236‑1 (MQ=255)
                                                                            aCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGGACGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCCTGTTGACCGCTGTGGCCGTGAAGCaaatctaaacttgg                                                                                                                                                       >  2:526481/1‑228 (MQ=255)
                                                                            aCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTggg                                                                                                                                                      >  1:167728/1‑235 (MQ=255)
                                                                            aCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGAACGTGATTGGCGGCCTGACCAACGTACTTGCCACCCTTATCGCAATCGGCCTTGTTGACACCTGGGGACGTAACACAAAGCTAACGCTggg                                                                                                                                                      >  2:397299/1‑235 (MQ=255)
                                                                            aCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGG‑GGGGGACCGTGATTGGGGGCCTGACCAAAGAACTTGCCACCTTTATTGCAATCGGCCTTGTTGACCGCTGGGGGCGCAAACCaacgctaaagcatgg                                                                                                                                                      >  2:493467/1‑231 (MQ=255)
                                                                            aCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAAACCTACCGAGCAAATTA‑GGGGGACCGTGGTTGGCGGCCTGACCAACGGCCTTTCCACCTTTATCGCCAACGGCCTTGTTTACCGCTGGGGACGTAAACCAACGCGAACGCTggg                                                                                                                                                      >  2:231429/1‑235 (MQ=255)
                                                                               gCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTggg                                                                                                                                                      <  1:73028/232‑1 (MQ=255)
                                                                               gCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTggg                                                                                                                                                      <  2:167728/232‑1 (MQ=255)
                                                                                    ttCCGCCGCGCGGTGTTCCTTGGCTTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTc                                                                                                                                                  <  2:61874/231‑1 (MQ=255)
                                                                                     tCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTg                                                                                                                                            <  1:596685/236‑1 (MQ=255)
                                                                                             gaggTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGgctgc                                                                                                                                    <  1:694723/234‑1 (MQ=255)
                                                                                                  gtTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCa                                                                                                                               <  1:556324/236‑1 (MQ=255)
                                                                                                       ttGGCGTTCTGTGGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGc                                                                                                                          <  2:668823/236‑1 (MQ=255)
                                                                                                          gCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTa                                                                                                                       <  1:43125/236‑1 (MQ=255)
                                                                                                           cGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCCATCGGCCTTGTTGACCGCTGGGGACGTAAACCCAAGCTAACGCCGGGCTTCCTGGTGATGGCTGCTGGCaagtgggcgc                                                                                                                      >  2:43125/1‑227 (MQ=255)
                                                                                                            caacgtttGCAGGTAAGGAATCAATTCCCCGGGATGAACGTCATCATGTATTGCGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACt                                                                                                                     <  2:703849/230‑1 (MQ=255)
                                                                                                             tACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTc                                                                                                                    >  1:688915/1‑236 (MQ=255)
                                                                                                               cTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACCCCGGGCTTCCTGGGGATGGCTGCTGGCATGGGCGTACTCgg                                                                                                                  >  1:146189/1‑236 (MQ=255)
                                                                                                                 gTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACGAACAATACCGAGCAAATGT‑GTGGGGCCGTGATTGGCGGCCTGACCATCGTACTAGCCACCTTTATCACAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCGGGCATGGGCGTACTCGGTa                                                                                                                <  1:33408/236‑1 (MQ=255)
                                                                                                                           aTGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACt                                                                                                                     <  1:400431/221‑1 (MQ=255)
                                                                                                                            tGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAcc                                                                                                                                                                     <  1:83963/172‑1 (MQ=255)
                                                                                                                            tGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAcc                                                                                                                                                                     >  2:83963/1‑172 (MQ=255)
                                                                                                                            tGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCa                                                                                                                               >  1:192242/1‑210 (MQ=255)
                                                                                                                            tGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCa                                                                                                                               <  2:192242/210‑1 (MQ=255)
                                                                                                                            tGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGGACAATGATGCat                                                                                                     >  1:421813/1‑236 (MQ=255)
                                                                                                                             gcagcaATTCACCGGGATGACCGTCATAATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCata                                                                                                    <  1:718087/236‑1 (MQ=255)
                                                                                                                              cagcaATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCata                                                                                                    <  1:214438/235‑1 (MQ=255)
                                                                                                                                  aaTTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGGTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTTCTGGCATGGGCGTACTCGGAACAATGATGCCTATCgg                                                                                                >  1:685878/1‑235 (MQ=255)
                                                                                                                                    ttCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAAAGCTGGGCTTCCTGGTGATGGCTGCTGGCAGTGGCGTAATCGGTACAAATATGCTTATCGGTa                                                                                              >  2:718087/1‑235 (MQ=255)
                                                                                                                                     tCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTAtt                                                                                            <  1:397299/236‑1 (MQ=255)
                                                                                                                                       cccGGGATTAACGTCATCATGTATTACGAGCCAAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCa                                                                                          <  2:130574/235‑1 (MQ=255)
                                                                                                                                                                aCGCTCCGAAAATCTTCGAACTGGCGGGTTATCCCAACCCTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtcgcc                                                                 <  2:146189/236‑1 (MQ=255)
                                                                                                                                                                      cGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCa                                                                                                                               >  1:206714/1‑169 (MQ=255)
                                                                                                                                                                      cGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCa                                                                                                                               <  2:206714/169‑1 (MQ=255)
                                                                                                                                                                         aaaTCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCa                                                                                                      <  2:427271/190‑1 (MQ=255)
                                                                                                                                                                         aaaTCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCa                                                                                                      >  1:427271/1‑190 (MQ=255)
                                                                                                                                                                              ttCGAACTGGCGGGTTATTCCAACCCTGCCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATgctgct                                                   <  1:231429/236‑1 (MQ=255)
                                                                                                                                                                                        cGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTAt                                                                                                                                                                                                         >  1:222789/1‑76 (MQ=255)
                                                                                                                                                                                        cGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTAt                                                                                                                                                                                                         <  2:222789/76‑1 (MQ=255)
                                                                                                                                                                                              ataCCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGt                                                                                  <  1:78301/189‑1 (MQ=255)
                                                                                                                                                                                                    acacTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGcc                             >  2:574635/1‑236 (MQ=255)
                                                                                                                                                                                                     cacTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGGACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGGATTTTGCCATCGGCATGCTGCTGATGATTATTGTCgggattgtca                            >  2:684668/1‑228 (MQ=255)
                                                                                                                                                                                                     cacTACCGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGGACTCGGTACAATGATGCATATCGGTCTTCACTCTCCGCCGGCGCa                                                                           >  2:78301/1‑189 (MQ=255)
                                                                                                                                                                                                      cttACCGAGCAAATGT‑GGGGGACCGGAATTGGCGGCCTGCCCAACTGACTTGCCACCTTTACCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCAt                           <  2:604968/234‑1 (MQ=255)
                                                                                                                                                                                                          ccGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGt                       >  1:457171/1‑236 (MQ=255)
                                                                                                                                                                                                          ccGAGCAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGAGGTTTATTGTCGGTTTTGCCATGAGt                       >  1:316946/1‑236 (MQ=255)
                                                                                                                                                                                                               cAAATGT‑GGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCgg                  >  1:137385/1‑236 (MQ=255)
                                                                                                                                                                                                                   tgt‑gGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCg              <  2:457171/236‑1 (MQ=255)
                                                                                                                                                                                                                     t‑gGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGcc                                                                                                                                                                                              <  1:94942/58‑1 (MQ=255)
                                                                                                                                                                                                                     t‑gGGGGACCGCGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGcc                                                                                                                                                                                              >  2:94942/1‑58 (MQ=255)
                                                                                                                                                                                                                        gggcACCGTGATTGCCTGCCTGACCAACGTACTGGCCACCTTGATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGa          <  1:101625/236‑1 (MQ=255)
                                                                                                                                                                                                                             ccGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTg      >  2:656742/1‑235 (MQ=255)
                                                                                                                                                                                                                              cGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTgg     <  2:137385/235‑1 (MQ=255)
                                                                                                                                                                                                                              cGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCCCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTGGCCATGAGTGCCGGTCCGCTGATTTg      >  1:567409/1‑234 (MQ=255)
                                                                                                                                                                                                                                 gATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTa  <  1:656742/235‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     
GAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGT‑GGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTA  >  NC_000913/3088941‑3089399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A