Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,095,466 T→G L123L (CTT→CTG)  yggS → UPF0001 family protein, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,095,4660TG97.3% 487.4 / 3.0 149L123L (CTT→CTG) yggSUPF0001 family protein, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (71/74);  total (73/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01

Only 100 of 149 total aligned reads displayed.

CCTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACT  >  NC_000913/3095370‑3095561
                                                                                                |                                                                                               
 cTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACg                                                                                             >  1:1829014/1‑100 (MQ=255)
 cTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACg                                                                                             <  2:2583904/100‑1 (MQ=255)
   ggtggCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTAACCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGtt                                                                                           >  1:1317808/1‑100 (MQ=255)
     tggCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCt                                                                                         <  1:545612/100‑1 (MQ=255)
      ggCAGAGCATTTCGACTGGTGTCATACCATCGTCCTTTTGCGCATCGCTATACGTCTCAACCACCCGCTCACGGCAGGCCTTCCCCCTCTGCACGCTCTg                                                                                        >  2:3453945/1‑100 (MQ=255)
      ggCAGAGCATTTCGACTGGTGTCATACCATCGACGGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTg                                                                                        <  2:3301543/100‑1 (MQ=255)
      ggCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTg                                                                                        <  2:3489411/100‑1 (MQ=255)
       gCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGa                                                                                       >  1:895955/1‑100 (MQ=255)
        cAGAGCATTTCGACTGGTGTCATACCATCGCCCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGAt                                                                                      <  2:102593/100‑1 (MQ=255)
        cAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTCCCCGTCTCAACGACCAGGGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGAt                                                                                      >  2:1587174/1‑100 (MQ=255)
        cAGAGCATTTCGACCGCTGTCATACCATCAACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGAt                                                                                      <  2:179785/100‑1 (MQ=255)
         agagCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGAtt                                                                                     >  2:2841559/1‑100 (MQ=255)
          gagCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTc                                                                                    <  1:557485/100‑1 (MQ=255)
          gagCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCACTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTc                                                                                    >  1:3471939/1‑100 (MQ=255)
             cATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCaaa                                                                                 <  2:3280496/100‑1 (MQ=255)
                 tCGACTGGTGTCATACCATCGACCGTTTGCGGATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTaa                                                                             <  2:2072771/100‑1 (MQ=255)
                 tCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTaa                                                                             <  2:2353071/100‑1 (MQ=255)
                  cGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAAc                                                                            <  1:1975116/100‑1 (MQ=255)
                    aCTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACAt                                                                          <  1:227037/100‑1 (MQ=255)
                    aCTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACAt                                                                          <  2:306566/100‑1 (MQ=255)
                     cTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACAtt                                                                         >  2:3405526/1‑100 (MQ=255)
                       ggTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAg                                                                       >  1:139989/1‑100 (MQ=255)
                        gtgtCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGt                                                                      >  1:3487334/1‑100 (MQ=255)
                        gtgtCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGt                                                                      >  2:2353175/1‑100 (MQ=255)
                         tgtCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCGCTCTGAACGTTCTGATTCAAATTAACATTAGtg                                                                     <  2:281455/100‑1 (MQ=255)
                         tgtCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGtg                                                                     >  2:2043695/1‑100 (MQ=255)
                          gtCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGtga                                                                    >  2:1070327/1‑100 (MQ=255)
                          gtCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGtga                                                                    >  2:1281923/1‑100 (MQ=255)
                          gtCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGtga                                                                    >  1:3209723/1‑100 (MQ=255)
                            cATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGtgatg                                                                  <  2:3860410/100‑1 (MQ=255)
                             aTACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGtgatga                                                                 >  2:692333/1‑100 (MQ=255)
                               aCCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGaaa                                                               >  1:3731717/1‑100 (MQ=255)
                               aCCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGaaa                                                               >  2:1314114/1‑100 (MQ=255)
                                 cATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAAc                                                             <  2:3411472/100‑1 (MQ=255)
                                 cATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAAc                                                             <  1:199665/100‑1 (MQ=255)
                                  aTCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGCACGTTCTGATTCAAATTAACATTAGTGATGAAAACa                                                            >  2:3668811/1‑100 (MQ=255)
                                     gACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTa                                                         >  1:2920501/1‑100 (MQ=255)
                                     gACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTa                                                         <  1:2776319/100‑1 (MQ=255)
                                     gACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTa                                                         >  1:2466996/1‑100 (MQ=255)
                                     gACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTa                                                         <  2:1369963/100‑1 (MQ=255)
                                     gACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTa                                                         <  1:1329891/100‑1 (MQ=255)
                                     gACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGGGATGAAAACAGTa                                                         >  1:3378429/1‑100 (MQ=255)
                                       ccGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAg                                                       <  1:878942/100‑1 (MQ=255)
                                        cGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGt                                                      >  2:374659/1‑100 (MQ=255)
                                          tttGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTcc                                                    >  2:3694940/1‑100 (MQ=255)
                                            tGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCgg                                                  <  2:2876710/100‑1 (MQ=255)
                                            tGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCgg                                                  >  1:1175332/1‑100 (MQ=255)
                                            tGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCgg                                                  <  1:2459772/100‑1 (MQ=255)
                                                 aTCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTc                                             <  2:430666/100‑1 (MQ=255)
                                                 aTCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTc                                             >  1:649055/1‑100 (MQ=255)
                                                 aTCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTc                                             >  1:3999897/1‑100 (MQ=255)
                                                    gCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAAc                                          <  1:2376308/100‑1 (MQ=255)
                                                     cTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGATAACAGTAAGTCCGGGATTCAACt                                         <  2:395220/100‑1 (MQ=255)
                                                     cTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACt                                         >  1:4028880/1‑100 (MQ=255)
                                                        cccGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGc                                      <  1:708684/100‑1 (MQ=255)
                                                        cccGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGc                                      <  1:3392498/100‑1 (MQ=255)
                                                        cccGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGc                                      <  1:2448054/100‑1 (MQ=255)
                                                        cccGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGc                                      <  2:2643593/100‑1 (MQ=255)
                                                           gTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGa                                   >  1:1584097/1‑100 (MQ=255)
                                                             ctcAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAAc                                 >  1:1096135/1‑100 (MQ=255)
                                                              tcAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAACCAGTAAGTCCGGGATTCAACTGGCTGAACt                                >  2:1283282/1‑100 (MQ=255)
                                                              tcAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACt                                <  2:1739345/100‑1 (MQ=255)
                                                               cAACGACCAGCGCCCGGCAGAACTTCCGCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGGCCGGGATTCAACTGGCTGAACTg                               <  2:426876/100‑1 (MQ=255)
                                                               cAACGACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTg                               >  2:2079783/1‑100 (MQ=255)
                                                                  cGACCAGCGCCCGGCAGAGCTGCCCCCTGTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGAc                            <  1:1667042/100‑1 (MQ=255)
                                                                   gACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACg                           >  2:2833007/1‑100 (MQ=255)
                                                                   gACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACg                           <  2:2163017/100‑1 (MQ=255)
                                                                   gACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACg                           <  1:431111/100‑1 (MQ=255)
                                                                   gACCAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACg                           <  1:1805908/100‑1 (MQ=255)
                                                                     ccAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAg                         >  2:3829075/1‑100 (MQ=255)
                                                                     ccAGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAg                         >  2:3153247/1‑100 (MQ=255)
                                                                       aGCGCCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCt                       >  1:2197732/1‑100 (MQ=255)
                                                                        gcgcCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTg                      <  2:273466/100‑1 (MQ=255)
                                                                         cgcCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTgg                     >  1:499424/1‑100 (MQ=255)
                                                                          gcCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGc                    >  2:2042552/1‑100 (MQ=255)
                                                                           cccGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCa                   >  1:2070869/1‑100 (MQ=255)
                                                                           cccGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCa                   >  1:1526020/1‑100 (MQ=255)
                                                                             cGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGc                 <  1:1871651/100‑1 (MQ=255)
                                                                               gCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGGGATGAAAACAGTAAGTCAGGGATTCAACTGGCTAAACTGGACGAGCTGGCAGCTg               >  1:3800477/1‑100 (MQ=255)
                                                                                cAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGc              <  2:2566736/100‑1 (MQ=255)
                                                                                cAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGc              <  1:3942010/100‑1 (MQ=255)
                                                                                cAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGc              >  2:1421236/1‑100 (MQ=255)
                                                                                cAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGc              >  1:2389643/1‑100 (MQ=255)
                                                                                 agtaCTTCCCCCTCTGAACGTTCTTAGTCAAATTAGCATTAGTGATGGAAACAGTAACTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCg             <  2:2456650/97‑1 (MQ=255)
                                                                                 aGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCg             <  1:1180359/100‑1 (MQ=255)
                                                                                 aGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCg             <  2:82306/100‑1 (MQ=255)
                                                                                  gAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCgg            >  2:2442111/1‑100 (MQ=255)
                                                                                  gAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCgg            <  2:2268573/100‑1 (MQ=255)
                                                                                    aCTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTc          >  1:570401/1‑100 (MQ=255)
                                                                                     cTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCg         >  1:3857795/1‑100 (MQ=255)
                                                                                       tCCCCCTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCt       <  1:2715208/100‑1 (MQ=255)
                                                                                        cccccTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCtn      <  1:3230537/100‑2 (MQ=255)
                                                                                        cccccTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTg      >  1:4064893/1‑100 (MQ=255)
                                                                                        cccccTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTg      <  1:555753/100‑1 (MQ=255)
                                                                                         ccccTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGa     >  1:3038846/1‑100 (MQ=255)
                                                                                         ccccTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGa     >  2:79781/1‑100 (MQ=255)
                                                                                          cccTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAGCTGGACGAGCTGGCAGCTGCGGTCGCTGaa    <  1:2426618/100‑1 (MQ=255)
                                                                                          cccTCTGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGaa    >  2:602590/1‑100 (MQ=255)
                                                                                            ctctGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACt  >  2:1075755/1‑100 (MQ=255)
                                                                                            ctctGAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACt  >  1:727741/1‑100 (MQ=255)
                                                                                                |                                                                                               
CCTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACT  >  NC_000913/3095370‑3095561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A