breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 6,809 T→G K384T (AAA→ACA)  yaaJ ← putative transporter
RA 25,474 T→G S90A (TCG→GCG)  lspA → prolipoprotein signal peptidase (signal peptidase II)
RA 76,539 T→G E254A (GAA→GCA)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 95,418 T→G S257A (TCG→GCG)  murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
JC JC 111,051 IS1 (–) +9 bp coding (8‑16/390 nt) mutT → dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
RA 133,452 A→C K613T (AAA→ACA)  acnB → aconitate hydratase 2; aconitase B; 2‑methyl‑cis‑aconitate hydratase
RA 142,754 A→C intergenic (‑84/‑25) can ← / → yadG carbonic anhydrase/putative ABC transporter ATPase
RA 143,263 A→C E162A (GAA→GCA)  yadG → putative ABC transporter ATPase
RA 162,349 A→C Y82S (TAC→TCC)  hrpB → putative ATP‑dependent helicase
RA 213,847 T→G E29A (GAG→GCG)  rof ← modulator of Rho‑dependent transcription termination
RA 243,021 T→G T95P (ACC→CCC)  fadE ← acyl coenzyme A dehydrogenase
RA 257,298 A→C N258H (AAC→CAC)  frsA → fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
MC JC 257,908 Δ776 bp [crl] [crl]
RA 299,214 A→C F574C (TTC→TGC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 301,463 T→G E142A (GAA→GCA)  paoB ← PaoABC aldehyde oxidoreductase, FAD‑containing subunit
RA 313,275 T→G intergenic (‑33/+441) ykgR ← / ← ykgP uncharacterized protein/pseudogene, oxidoreductase family
RA 316,817 T→G pseudogene (353/684 nt) ykgA ← pseudogene, AraC family;putative regulator; Not classified; putative ARAC‑type regulatory protein
RA 366,811 T→G N234H (AAT→CAT)  lacI ← lactose‑inducible lac operon transcriptional repressor
RA 394,041 T→G pseudogene (1072/1449 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
RA 400,871 T→G E18A (GAA→GCA)  ddlA ← D‑alanine‑D‑alanine ligase A
RA 402,002 T→G Y86D (TAT→GAT)  phoA → bacterial alkaline phosphatase
RA 463,948 A→C G4G (GGA→GGC ybaV → putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein
RA 480,566 A→C L48W (TTG→TGG)  tomB ← Hha toxicity attenuator; conjugation‑related protein
RA 491,048 T→G F56C (TTT→TGT)  ybaN → DUF454 family inner membrane protein
RA 519,286 A→C V160V (GTT→GTG tesA ← acyl‑CoA thioesterase 1 and protease I and lysophospholipase L1
RA 546,173 T→G E64A (GAA→GCA)  allD ← ureidoglycolate dehydrogenase
RA 568,937 A→C S12S (TCA→TCC ybcK → DLP12 prophage; putative recombinase
RA 570,560 A→C intergenic (+132/‑333) ybcK → / → ybcL DLP12 prophage; putative recombinase/inactive polymorphonuclear leukocyte migration suppressor; DLP12 prophage; UPF0098 family secreted protein
RA 576,137 T→G pseudogene (689/1040 nt) nmpC ← DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
RA 611,121 A→C S458R (AGT→AGG fepA ← ferrienterobactin outer membrane transporter
RA 615,736 A→C E527A (GAA→GCA)  entF → enterobactin synthase multienzyme complex component, ATP‑dependent
RA 619,006 A→C G251G (GGA→GGC fepE → regulator of length of O‑antigen component of lipopolysaccharide chains
RA 623,458 T→G L387V (TTG→GTG)  entS → enterobactin exporter, iron‑regulated
RA 650,553 T→G A77A (GCA→GCC citD ← citrate lyase, acyl carrier (gamma) subunit
RA 653,352 A→C Q373P (CAG→CCG)  citA → sensory histidine kinase in two‑component regulatory system with CitB
RA 733,589 T→G L1336* (TTA→TGA)  rhsC → Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
RA 741,597 T→G A320A (GCA→GCC dtpD ← dipeptide and tripeptide permease D
RA 754,738 A→C intergenic (‑270/‑439) gltA ← / → sdhC citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
RA 783,757 A→C I198L (ATT→CTT)  pnuC → nicotinamide riboside transporter
RA 798,817 T→G S78A (TCT→GCT)  pgl → 6‑phosphogluconolactonase
RA 825,545 T→G V188V (GTA→GTC ybhR ← putative ABC transporter permease
RA 846,198 A→C F89C (TTC→TGC)  glnQ ← glutamine transporter subunit
RA 917,078 T→G D202E (GAT→GAG ybjD → putative OLD family ATP‑dependent endonuclease; DUF2813 family protein
RA 921,236 A→C K297T (AAA→ACA)  macB → macrolide ABC transporter peremase/ATPase
RA 924,571 T→G I436M (ATT→ATG clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 930,957 A→C N2K (AAT→AAG cydD ← glutathione/cysteine ABC transporter export permease/ATPase
RA 954,603 T→G intergenic (‑137/+269) focA ← / ← ycaO formate channel/ribosomal protein S12 methylthiotransferase accessory factor
RA 960,657 C→T L132L (CTG→TTG)  ycaL → putative peptidase‑related chaperone
RA 987,767 T→G M407L (ATG→CTG)  asnS ← asparaginyl tRNA synthetase
RA 1,035,960 T→G L64R (CTC→CGC)  hyaD → hydrogenase 1 maturation protease
RA 1,048,134 A→C N186K (AAT→AAG gfcC ← putative O‑antigen capsule production periplasmic protein
RA 1,078,882 T→G M1M (ATG→CTG) † putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
RA 1,091,002 T→G N430H (AAC→CAC)  pgaA ← biofilm adhesin polysaccharide PGA secretin; OM porin; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein
RA 1,114,259 A→C intergenic (‑78/+5) msyB ← / ← mdtG multicopy suppressor of secY and secA/putative drug efflux system protein
RA 1,134,118 A→C Q106P (CAG→CCG)  flgF → flagellar component of cell‑proximal portion of basal‑body rod
RA 1,139,916 T→G D513E (GAT→GAG flgK → flagellar hook‑filament junction protein 1
RA 1,183,048 T→G L191F (TTA→TTC potC ← spermidine/putrescine ABC transporter permease
RA 1,192,380 T→G E84D (GAA→GAC hflD ← putative lysogenization regulator
RA 1,224,314 T→G N78H (AAC→CAC)  minE ← cell division topological specificity factor
RA 1,256,574 T→G intergenic (‑622/+147) ycgV ← / ← ychF putative adhesin/catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated
RA 1,290,933 T→G L231R (CTT→CGT)  rssB → PcnB‑degradosome interaction factor; response regulator
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,325,833 T→G V363G (GTC→GGC)  yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,326,908 A→C E19D (GAA→GAC rluB → 23S rRNA pseudouridine(2605) synthase
RA 1,343,516 A→C intergenic (‑188/+81) osmB ← / ← yciT osmotically and stress inducible lipoprotein/global regulator of transcription; DeoR family
RA 1,424,158 T→G L126* (TTA→TGA)  trkG → Rac prophage; potassium transporter subunit
RA 1,432,583 T→G F58C (TTC→TGC)  tfaR → Rac prophage; putative tail fiber assembly protein
RA 1,438,947 T→G E613A (GAA→GCA)  pfo ← pyruvate‑flavodoxin oxidoreductase
RA 1,461,931 A→C N347H (AAC→CAC)  paaJ → 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
RA 1,479,936 A→C N267H (AAC→CAC)  ynbC → putative esterase
RA 1,486,556 A→C I1166L (ATC→CTC)  hrpA → putative ATP‑dependent helicase
RA 1,546,032 A→C intergenic (‑4/+256) yddL ← / ← yddG putative lipoprotein;putative membrane; Not classified; putative outer membrane porin protein/aromatic amino acid exporter
RA 1,562,611 A→C F156V (TTT→GTT)  ddpX ← D‑ala‑D‑ala dipeptidase, Zn‑dependent
RA 1,571,816 T→G D77A (GAT→GCT)  gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,575,705 T→G N639H (AAC→CAC)  yddB ← putative TonB‑dependent outer membrane receptor
RA 1,597,634 A→C pseudogene (354/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,676,734 T→G K390N (AAA→AAC pntA ← pyridine nucleotide transhydrogenase, alpha subunit
RA 1,681,460 T→G M162R (ATG→AGG)  folM → dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme
RA 1,714,643 T→G I89M (ATT→ATG gstA → glutathionine S‑transferase
RA 1,742,506 T→G L365R (CTC→CGC)  cfa → cyclopropane fatty acyl phospholipid synthase, SAM‑dependent
RA 1,756,929 T→G L411R (CTG→CGG)  pykF → pyruvate kinase I
RA 1,815,451 T→G F529V (TTT→GTT)  katE → catalase HPII, heme d‑containing
RA 1,822,043 T→G I72L (ATC→CTC)  osmE ← osmotically‑inducible lipoprotein
RA 1,830,953 T→G N344H (AAT→CAT)  astC ← succinylornithine transaminase, PLP‑dependent
RA 1,869,873 T→G F307V (TTC→GTC)  yeaH → UPF0229 family protein
RA 1,896,050 A→C N416H (AAC→CAC)  pabB → aminodeoxychorismate synthase, subunit I
RA 1,921,059 T→G P279P (CCT→CCG rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
RA 1,927,578 A→C I421R (ATA→AGA)  ptrB ← protease II
RA 1,962,412 A→C L147L (CTA→CTC yecT → uncharacterized protein
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,008,526 A→C I84L (ATC→CTC)  yedE → UPF0394 family sulphur transport domain‑containing inner membrane protein
RA 2,010,994 A→C D132A (GAT→GCT)  yedL → GNAT family putative N‑acetyltransferase
RA 2,020,656 T→G N190K (AAT→AAG fliM → flagellar motor switching and energizing component
RA 2,021,050 A→C N322H (AAC→CAC)  fliM → flagellar motor switching and energizing component
RA 2,036,444 T→G Y204D (TAT→GAT)  hchA → glyoxalase III and Hsp31 molecular chaperone
RA 2,075,512 T→G L245R (CTG→CGG)  yeeR → CP4‑44 prophage; putative membrane protein
RA 2,077,040 T→G intergenic (+2/‑72) yeeT → / → cbeA CP4‑44 prophage; uncharacterized protein/CP4‑44 prophage; cytoskeleton bundling‑enhancing factor A; CbtA antitoxin
RA 2,110,741 A→C F93L (TTT→TTG rfbA ← glucose‑1‑phosphate thymidylyltransferase
RA 2,127,751 T→G E188D (GAA→GAC gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding
RA 2,151,093 T→G intergenic (‑108/‑92) yegI ← / → yegJ protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein
RA 2,170,272 A→C K13Q (AAA→CAA)  insE1 → IS3 transposase A
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,179,614 T→G F265C (TTC→TGC)  yegT → nucleoside transporter, low affinity
RA 2,185,809 T→G intergenic (‑13/‑108) rcnR ← / → rcnA transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance
RA 2,208,622 T→G L219* (TTA→TGA)  yehP → VMA domain putative YehL ATPase stimulator
RA 2,221,860 T→G K44Q (AAA→CAA)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,273,796 A→C D478A (GAC→GCC)  yejA → microcin C ABC transporter periplasmic binding protein
RA 2,278,486 T→G intergenic (‑249/+84) yejG ← / ← bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
RA 2,281,112 A→C I161L (ATC→CTC)  yejH → putative ATP‑dependent DNA or RNA helicase
RA 2,292,894 A→C L24R (CTG→CGG)  dsbE ← periplasmic thioredoxin of cytochrome c‑type biogenesis
RA 2,318,639 A→C F413C (TTT→TGT)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,323,353 T→G intergenic (+102/‑94) atoC → / → atoD fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
RA 2,387,305 G→A P124P (CCG→CCA yfbM → DUF1877 family protein
RA 2,395,394 A→C L168R (CTG→CGG)  nuoJ ← NADH:ubiquinone oxidoreductase, membrane subunit J
RA 2,398,075 A→C I697M (ATT→ATG nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,421,142 T→G T174T (ACT→ACG yfcG → GSH‑dependent disulfide bond oxidoreductase
RA 2,488,822 A→C L116R (CTT→CGT)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase
RA 2,503,731 T→G E252A (GAA→GCA)  fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,518,687 A→C E74A (GAA→GCA)  yfeC → DUF1323 family putative DNA‑binding protein
RA 2,547,053 T→G G127G (GGT→GGG murP → N‑acetylmuramic acid permease, EIIBC component, PTS system
RA 2,589,778 T→G D728E (GAT→GAG acrD → aminoglycoside/multidrug efflux system
RA 2,624,901 T→G Y620D (TAT→GAT)  ppk → polyphosphate kinase, component of RNA degradosome
RA 2,668,449 A→C Y150D (TAT→GAT)  hcaR ← hca operon transcriptional regulator
RA 2,717,422 A→C V223V (GTA→GTC ung → uracil‑DNA‑glycosylase
RA 2,769,390 A→C R242R (CGA→CGC yfjQ → CP4‑57 prophage; uncharacterized protein
RA 2,869,491 A→C L10R (CTG→CGG)  pcm ← L‑isoaspartate protein carboxylmethyltransferase type II
RA 2,899,375 A→C I15S (ATT→AGT)  ygcU ← putative FAD‑linked oxidoreductase
RA 2,904,306 T→G intergenic (+932/+441) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,912,404 A→C V416V (GTT→GTG relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,934,721 T→G S163A (TCG→GCG)  fucP → L‑fucose transporter
RA 2,942,844 T→G noncoding (149/206 nt) gcvB → sRNA antisense regulator represses oppA, dppA, gltI and livJ expression, Hfq‑dependent
RA 2,956,720 T→G K722T (AAA→ACA)  ptrA ← protease III
RA 2,968,180 T→G I85I (ATA→ATC ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 3,009,624 A→C K287N (AAA→AAC ygeY → putative peptidase
RA 3,014,506 T→G Q184H (CAA→CAC yqeC ← putative selenium‑dependent hydroxylase accessory protein
RA 3,040,512 A→C G104G (GGT→GGG cptA ← toxin of CptAB toxin‑antitoxin pair
RA 3,044,628 A→C K323Q (AAG→CAG)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,052,652 T→G Q289H (CAA→CAC ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
RA 3,082,025 A→C Y38S (TAC→TCC)  loiP → Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein
RA 3,089,170 T→G V296G (GTC→GGC)  galP → D‑galactose transporter
RA 3,101,594 T→G A10A (GCA→GCC yggN ← DUF2884 family putative periplasmic protein
RA 3,123,491 A→C I105M (ATT→ATG glcB ← malate synthase G
RA 3,174,035 T→G E454D (GAA→GAC parE ← DNA topoisomerase IV, subunit B
RA 3,174,612 A→C L262R (CTG→CGG)  parE ← DNA topoisomerase IV, subunit B
RA 3,182,529 A→C intergenic (‑122/‑21) ygiD ← / → zupT 4,5‑ DOPA‑extradiol‑dioxygenase/zinc transporter
RA 3,197,496 T→G T716P (ACT→CCT)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,198,644 A→C F333C (TTC→TGC)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,271,880 T→G E291A (GAA→GCA)  garR ← tartronate semialdehyde reductase
RA 3,290,794 A→C I661L (ATC→CTC)  yraJ → putative outer membrane protein
RA 3,327,851 T→G V21G (GTT→GGT)  yhbY → RNA binding protein associated with pre‑50S ribosomal subunits
RA 3,393,985 A→C P758P (CCT→CCG yhdP ← DUF3971‑AsmA2 domains protein
RA 3,399,779 A→C L67R (CTG→CGG)  mreC ← cell wall structural complex MreBCD transmembrane component MreC
RA 3,408,510 A→C T302P (ACC→CCC)  panF → pantothenate:sodium symporter
RA 3,439,399 A→C S37S (TCT→TCG yhdN ← DUF1992 family protein
RA 3,452,188 A→C V33V (GTT→GTG rplD ← 50S ribosomal subunit protein L4
RA 3,468,138 T→G G572G (GGA→GGC chiA ← periplasmic endochitinase
RA 3,473,612 A→C L157* (TTA→TGA)  rpsG ← 30S ribosomal subunit protein S7
RA 3,488,471 A→C N561H (AAC→CAC)  yhfK → putative transporter, FUSC superfamily inner membrane protein
RA 3,515,115 A→C L267V (TTG→GTG)  dam ← DNA adenine methyltransferase
RA 3,519,300 T→G intergenic (‑236/+165) aroK ← / ← hofQ shikimate kinase I/DNA catabolic putative fimbrial transporter
RA 3,520,438 A→C L89R (CTC→CGC)  hofQ ← DNA catabolic putative fimbrial transporter
RA 3,540,754 T→G I116M (ATT→ATG feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein
RA 3,559,559 T→G I431S (ATC→AGC)  rtcR → sigma 54‑dependent transcriptional regulator of rtcBA expression
RA 3,559,766 A→C Q500P (CAG→CCG)  rtcR → sigma 54‑dependent transcriptional regulator of rtcBA expression
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,577,196 T→G M133L (ATG→CTG)  gntK ← gluconate kinase 2
RA 3,600,185 T→G Y200S (TAT→TCT)  rpoH ← RNA polymerase, sigma 32 (sigma H) factor
RA 3,602,989 T→G I419L (ATT→CTT)  ftsY ← Signal Recognition Particle (SRP) receptor
RA 3,623,709 T→G I104S (ATT→AGT)  yhhH → putative NTF2 fold immunity protein for polymorphic toxin RhsB
RA 3,633,028 T→G pseudogene (238/414 nt) yhiL ← pseudogene, DUF4049 family protein
RA 3,662,163 A→C N584H (AAC→CAC)  mdtF → anaerobic multidrug efflux transporter, ArcA‑regulated
RA 3,679,181 T→G R3R (AGG→CGG)  yhjH ← cyclic‑di‑GMP phosphodiesterase, FlhDC‑regulated
RA 3,689,996 A→C L89* (TTA→TGA)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,697,854 A→C Q466P (CAA→CCA)  bcsE → cellulose production protein
RA 3,708,080 T→G intergenic (‑375/+536) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,710,019 T→G T153P (ACC→CCC)  eptB ← KDO phosphoethanolamine transferase, Ca(2+)‑inducible
RA 3,714,488 A→C L636W (TTG→TGG)  bisC ← biotin sulfoxide reductase
RA 3,716,242 A→C V51V (GTT→GTG bisC ← biotin sulfoxide reductase
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine tRNA synthetase, alpha subunit
RA 3,749,326 A→C D32A (GAT→GCT)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,752,058 A→C I23L (ATC→CTC)  yiaU → putative DNA‑binding transcriptional regulator
RA 3,790,919 A→C L143V (TTA→GTA)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding
RA 3,802,005 T→G intergenic (‑6/+34) waaR ← / ← waaO lipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase/UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑ alpha‑1,3‑D‑galactosyltransferase
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 3,823,774 A→C N459H (AAC→CAC)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,913,683 A→C intergenic (‑15/+147) glmS ← / ← glmU L‑glutamine:D‑fructose‑6‑phosphate aminotransferase/fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
RA 3,939,836 A→C F259L (TTT→TTG hsrA ← putative multidrug or homocysteine efflux system
RA 3,966,875 T→G S153S (TCT→TCG rho → transcription termination factor
RA 3,972,452 A→C Q398P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
RA 4,008,222 A→C I156L (ATC→CTC)  rhtC → threonine efflux pump
RA 4,012,707 A→C F37C (TTT→TGT)  metR ← methionine biosynthesis regulon transcriptional regulator
RA 4,024,756 T→G E22D (GAA→GAC rfaH ← transcription antitermination protein
RA 4,031,324 A→C Q55P (CAA→CCA)  pepQ → proline dipeptidase
RA 4,046,123 A→C H160Q (CAT→CAG yihG ← inner membrane protein, inner membrane acyltransferase
RA 4,046,716 A→C intergenic (‑114/‑250) yihG ← / → polA inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
RA 4,061,235 A→C Y24S (TAT→TCT)  yihM → putative sugar phosphate isomerase
RA 4,117,812 T→G Q93H (CAA→CAC glpF ← glycerol facilitator
RA 4,146,625 T→G L123R (CTT→CGT)  pflC → putative [formate‑C‑acetyltransferase 2]‑activating enzyme; pyruvate formate‑lyase 1‑activating enzyme
RA 4,186,603 A→C E418D (GAA→GAC rpoC → RNA polymerase, beta prime subunit
RA 4,190,100 T→G I105M (ATT→ATG yjaZ → stationary phase growth adaptation protein
RA 4,215,122 T→G G281G (GGT→GGG metA → homoserine O‑transsuccinylase
RA 4,222,536 A→C intergenic (‑49/+268) arpA ← / ← iclR ankyrin repeat protein/transcriptional repressor
RA 4,229,644 T→G K167Q (AAA→CAA)  pepE ← peptidase E, alpha‑aspartyl dipeptidase
RA 4,231,659 T→G intergenic (‑28/+225) yjbD ← / ← lysC DUF3811 family protein/lysine‑sensitive aspartokinase 3
RA 4,260,939 T→G I114S (ATT→AGT)  yjbM → uncharacterized protein
RA 4,285,713 A→C N553K (AAT→AAG acs ← acetyl‑CoA synthetase
RA 4,291,339 T→G I288M (ATT→ATG nrfD → formate‑dependent nitrite reductase, membrane subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,307,372 A→C M143R (ATG→AGG)  alsK ← D‑allose kinase
RA 4,313,090 T→G intergenic (‑99/‑260) alsR ← / → rpiB d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,358,516 A→C V34V (GTT→GTG cadA ← lysine decarboxylase, acid‑inducible
RA 4,403,148 A→C N371H (AAC→CAC)  hflK → modulator for HflB protease specific for phage lambda cII repressor
RA 4,408,287 A→C Q545P (CAG→CCG)  rnr → exoribonuclease R, RNase R
RA 4,420,658 A→C N227H (AAC→CAC)  ulaA → L‑ascorbate‑specific enzyme IIC permease component of PTS
RA 4,475,797 A→C I121L (ATT→CTT)  yjgL → SopA‑central‑domain‑like hexapeptide repeat protein
RA 4,477,084 A→C N550H (AAT→CAT)  yjgL → SopA‑central‑domain‑like hexapeptide repeat protein
RA 4,522,275 T→G H607P (CAT→CCT)  yjhG ← putative dehydratase
RA 4,548,617 A→C L111L (CTA→CTC fimG → minor component of type 1 fimbriae
RA 4,559,230 T→G S99S (TCA→TCC iadA ← isoaspartyl dipeptidase
RA 4,566,805 T→G I148L (ATT→CTT)  yjiN ← zinc‑type alcohol dehydrogenase‑like protein
RA 4,595,235 A→C F206C (TTT→TGT)  lgoR ← putative transcriptional activator for L‑galactonate catabolism
RA 4,613,561 C→T R27R (CGC→CGT yjjV → putative DNase
RA 4,628,633 A→C intergenic (+86/+222) nadR → / ← ettA trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor/energy‑dependent translational throttle A

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423836–3424233 3424473–3424238 6–638 21 [20] [20] 21 [rrlD] [rrlD]
* * ÷ NC_000913 4472196 4472259 64 21 [19] [7] 28 pyrB aspartate carbamoyltransferase, catalytic subunit

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 19785020 (0.000)64 (1.000) 50/410 0.2 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein