Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I51 R1	223	69.9	1534784	95.1%	1459579	214.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,913,683	A→C	intergenic (‑15/+147)	glmS ← / ← glmU	L‑glutamine:D‑fructose‑6‑phosphate aminotransferase/fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,913,683	0	A	C	100.0%	188.5 / NA	54	intergenic (‑15/+147)	glmS/glmU	L‑glutamine:D‑fructose‑6‑phosphate aminotransferase/fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (26/28); total (26/28)

TGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGAC  >  NC_000913/3913454‑3913910

tgAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGGATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCGACAT‑CACCTTTCGCGATCCCGCCCACAATTCCACACCTAGCTTTTTATTCCGCTTtata                                                                                                                                                                                                                                >  2:480305/1‑236 (MQ=255)

           aGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGt                                                                                                                                                                                                                     <  1:705047/236‑1 (MQ=255)

               ccgccATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCCGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACCTTTGGCGATCGCGCCAACAATTTCACACCTAGTTTTTGATTTCGCTTTATATCGTTTTCGGt                                                                                                                                                                                                                     >  1:640772/1‑232 (MQ=255)

                             aTGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑c                                                                                                                                                                                                        <  1:475108/230‑1 (MQ=255)

                                      cGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCCTATCCCCGGTATTCCAGACGACGTAAACCCTCAAGCAGGATTTCTGCTACATCCCCGTTG‑GCGATCTGGCCAACAATTTCACACATAGATTTTGGTTCCCCCTTTTATCGTTGTCGGTCCACCCGTTTGTCCCGttt                                                                                                                                                                                                  >  2:539778/1‑228 (MQ=255)

                                            ttgtgCAGGCATCTGGACTTGACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGa                                                                                                                                                                                   <  2:392287/235‑1 (MQ=255)

                                            cTGTGCCGACATCTGGACTTTACCGAGGCGACGCAGGCGGTTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGa                                                                                                                                                                                   <  2:294775/236‑1 (MQ=255)

                                               tgCCAGCATCTGGCTTTTCCCGAGGCGACGCAGGCGGGTCATATTACCTTCTGCATCAACAACGGCCAGCCCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTAAAGAAGGATTGCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGcc                                                                                                                                                                                <  1:335716/236‑1 (MQ=255)

                                               gcccatgtgttGGACTTTCCCGAGGCGAAGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGcc                                                                                                                                                                                <  2:311247/226‑1 (MQ=255)

                                                         tGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTaga                                                                                                                                                                         <  1:515141/233‑1 (MQ=255)

                                                         tGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTa                                                                                                                                                                           <  1:199752/231‑1 (MQ=255)

                                                                tACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAAACCGGCCGAGGCATATCCGCGGGATTCCAGACGACGGAAACCTTTCAGAAGGACTTCTGTTACAT‑CACGTTGCGCGGTCGGGCCTACAAATCCACACATAGTTTTTGAGTTCGCTTTTTATCTTTGTCGGTCAACCTGTATG‑CCCGCGTTTTTTGGGGGCCCGGGCCTCGTTGAAAGTGTGGtg                                                                                                                                                               >  2:584458/1‑235 (MQ=255)

                                                                   cGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGa                                                                                                                                                                                   <  1:751984/213‑1 (MQ=255)

                                                                      ggcgtagCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTCCAT‑CACGTTGCGCGATCGCGCCAACAATTCCACCCATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTAttttt                                                                                                                                                         <  1:584458/230‑1 (MQ=255)

                                                                            gCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGt                                                                                                                                                    <  1:125666/235‑1 (MQ=255)

                                                                                       atatGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTgg                                                                                                                                        >  1:435328/1‑236 (MQ=255)

                                                                                        tatGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGa                                                                                                                                                                                   >  2:333112/1‑192 (MQ=255)

                                                                                        tatGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGa                                                                                                                                                                                   <  1:333112/192‑1 (MQ=255)

                                                                                          tggccTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTgggag                                                                                                                                     <  1:179080/233‑1 (MQ=255)

                                                                                          tGACCTTCTGAATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTgggag                                                                                                                                     <  1:220438/236‑1 (MQ=255)

                                                                                                tCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCTGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGAt                                                                                                                               >  2:571498/1‑236 (MQ=255)

                                                                                                                    aCCGGCAGATTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCAt                                                                                                               <  1:118328/232‑1 (MQ=255)

                                                                                                                    aCCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTggga                                                                                                                                      >  1:251879/1‑209 (MQ=255)

                                                                                                                    aCCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTggga                                                                                                                                      <  2:251879/209‑1 (MQ=255)

                                                                                                                    aCCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCAt                                                                                                               <  2:435328/232‑1 (MQ=255)

                                                                                                                              tCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGAATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGAGAGATTATGTTCTCTCCTCATACCATGTGACAggg                                                                                                 >  2:109267/1‑236 (MQ=255)

                                                                                                                                       cgGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGcccc                                                                                                                                                                                     >  1:162476/1‑143 (MQ=255)

                                                                                                                                       cgGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGcccc                                                                                                                                                                                     <  2:162476/143‑1 (MQ=255)

                                                                                                                                        ggTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGt                                                                                                                                                                                                                     <  1:690128/111‑1 (MQ=255)

                                                                                                                                        ggTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGt                                                                                                                                                                                                                     >  2:690128/1‑111 (MQ=255)

                                                                                                                                                    gacgTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTACTCATCCCATGTGACCGGCGTAGCCGGCCAGAATCCAtttt                                                                           >  2:391289/1‑236 (MQ=255)

                                                                                                                                                      cgTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGcccc                                                                                                                                                                                     >  1:17491/1‑128 (MQ=255)

                                                                                                                                                      cgTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGcccc                                                                                                                                                                                     <  2:17491/128‑1 (MQ=255)

                                                                                                                                                      cgTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTa                                                                                                                        >  2:430649/1‑189 (MQ=255)

                                                                                                                                                      cgTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTa                                                                                                                        <  1:430649/189‑1 (MQ=255)

                                                                                                                                                      cgTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACttcttc                                                                         >  1:711727/1‑236 (MQ=255)

                                                                                                                                                      cgTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACt                                                                              >  1:92998/1‑231 (MQ=255)

                                                                                                                                                      cgTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTc                                                                         >  1:699956/1‑236 (MQ=255)

                                                                                                                                                               tCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGga                                                                <  1:571498/236‑1 (MQ=255)

                                                                                                                                                                       gATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGCCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCTTCATCCCATCTGACCGGGTTACCCGGCCAGAATCACTTTTTCTTTACCGGACGACGc                                                          >  2:174059/1‑234 (MQ=255)

                                                                                                                                                                            cTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCctt                                                   <  1:391289/236‑1 (MQ=255)

                                                                                                                                                                                             cgcgATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTTAGtatccg                                    >  2:316731/1‑230 (MQ=255)

                                                                                                                                                                                             cgcgATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCgg                                   >  1:228773/1‑236 (MQ=255)

                                                                                                                                                                                               cgATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTg                                      <  2:755445/231‑1 (MQ=255)

                                                                                                                                                                                               cgATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTg                                      >  1:755445/1‑231 (MQ=255)

                                                                                                                                                                                                gATCGCTCAAACATTTCCACACATTGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAg                                                       <  2:201063/213‑1 (MQ=255)

                                                                                                                                                                                                  acgatccAACAGTTCCACACATATGTGTTGATGCCGCTTTATATCGTTGTCGGTCAGCCTGTATG‑CCCGTTATTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGcaca                               <  2:640772/230‑1 (MQ=255)

                                                                                                                                                                                                       ccAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCcttctt                                                >  1:201063/1‑213 (MQ=255)

                                                                                                                                                                                                       ccAACAATTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTg                         <  1:316731/236‑1 (MQ=255)

                                                                                                                                                                                                           caaTTCCACACATAGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAg                     <  2:228773/236‑1 (MQ=255)

                                                                                                                                                                                                                        aGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGCTAGCTATTGCActttg        >  2:332670/1‑235 (MQ=255)

                                                                                                                                                                                                                        aGTTTTTGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCa             >  1:732293/1‑231 (MQ=255)

                                                                                                                                                                                                                            tttGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCg    >  2:201271/1‑236 (MQ=255)

                                                                                                                                                                                                                              tGATTCCGCTTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGAc  >  1:512016/1‑236 (MQ=255)

TGAGCAATACCAGTGCCGCCATGCAGAGGATGTTCTTCCGCTGCCTGTGCCAGCATCTGGACTTTACCGAGGCGACGCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACAT‑CACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATG‑CCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGAC  >  NC_000913/3913454‑3913910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A