Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,271,880 T→G E291A (GAA→GCA)  garR ← tartronate semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,271,8800TG98.9% 642.5 / ‑3.0 182E291A (GAA→GCA) garRtartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (100/80);  total (102/80)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 182 total aligned reads displayed.

TACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACC  >  NC_000913/3271783‑3271975
                                                                                                 |                                                                                               
 aCCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGa                                                                                              >  2:1940072/1‑100 (MQ=255)
  ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGAc                                                                                             >  2:2367677/1‑100 (MQ=255)
  ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGAc                                                                                             >  1:2843825/1‑100 (MQ=255)
  ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGAc                                                                                             >  2:523763/1‑100 (MQ=255)
  ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGAc                                                                                             <  2:439202/100‑1 (MQ=255)
      aTGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTc                                                                                         >  1:2561346/1‑100 (MQ=255)
       tGACGCCTGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCg                                                                                        <  2:1074428/100‑1 (MQ=255)
       tGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCg                                                                                        >  1:2624310/1‑100 (MQ=255)
         aCGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGcc                                                                                      <  1:1259882/100‑1 (MQ=255)
         aCGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGcc                                                                                      >  1:3780060/1‑100 (MQ=255)
            ctggAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGt                                                                                   <  2:3641125/98‑1 (MQ=255)
            ccGGAGGGCGTTGCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGt                                                                                   <  1:2507314/100‑1 (MQ=255)
              ggAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGttt                                                                                 <  2:223634/100‑1 (MQ=255)
               gAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGtttt                                                                                <  2:979676/100‑1 (MQ=255)
                  ggCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCg                                                                             <  1:3844984/100‑1 (MQ=255)
                   gCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgt                                                                            <  1:2912681/100‑1 (MQ=255)
                   gCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgt                                                                            <  2:3661415/100‑1 (MQ=255)
                   gCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgt                                                                            <  1:3196183/100‑1 (MQ=255)
                   gCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgt                                                                            <  1:3615130/100‑1 (MQ=255)
                   gCGTTTCTCTTATTCGGCCTGGATCCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgt                                                                            >  1:1722313/1‑100 (MQ=255)
                     gTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgtag                                                                          >  2:146244/1‑100 (MQ=255)
                     gTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgtag                                                                          >  1:2666994/1‑100 (MQ=255)
                      tttCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGTCAGTTTTTCgtagt                                                                         <  2:1780329/100‑1 (MQ=255)
                       ttCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCgtagta                                                                        >  2:1289393/1‑100 (MQ=255)
                         ctctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGc                                                                      <  1:1971195/100‑1 (MQ=255)
                           ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCAc                                                                    >  1:984702/1‑100 (MQ=255)
                             tATTCGGCCTGGATTCCAGGCCCGGATTTCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTGCGCCAGTTTTTCGTAGTAGCACGc                                                                  >  2:2862988/1‑100 (MQ=255)
                              aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGcc                                                                 >  2:2621340/1‑100 (MQ=255)
                              aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGcc                                                                 >  2:1674017/1‑100 (MQ=255)
                               ttCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCa                                                                >  2:1231734/1‑100 (MQ=255)
                               ttCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCa                                                                <  1:613260/100‑1 (MQ=255)
                               ttCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCa                                                                >  1:401214/1‑100 (MQ=255)
                               ccgggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCa                                                                >  1:366074/4‑100 (MQ=255)
                                tCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAg                                                               <  1:3282375/100‑1 (MQ=255)
                                tCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAg                                                               <  1:3772832/100‑1 (MQ=255)
                                cgggCCTGGAATCCAGGCCCGGATTGCAATACGCTATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAg                                                               >  2:2502397/3‑100 (MQ=255)
                                 gggCCTGGAATCCAAGCCCGGATTGCAATCCGCAATCCGGGCACGACGTCATTAACGAGTAACTGCAACCTTCGCCAGTTTTTCGTAGTAACACGCCAga                                                              >  2:3597124/2‑99 (MQ=255)
                                  ggCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAggg                                                             >  1:3472758/1‑100 (MQ=255)
                                  ggCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAggg                                                             <  1:1116471/100‑1 (MQ=255)
                                  ggCCTGGATTCCAGGCCCGGATTGCAATACGCCAGCCGGGCACGACGGCATTAACGAGTAACTGCGATTTTCGCCAGTTTTTCGTAGTAGCACGCCAggg                                                             <  1:1315756/100‑1 (MQ=255)
                                  ggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAggg                                                             <  2:1476627/100‑1 (MQ=255)
                                   gCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgc                                                            <  1:4049239/100‑1 (MQ=255)
                                    ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgcg                                                           >  1:2114602/1‑100 (MQ=255)
                                     cTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTCGCACGCCAGGgcgc                                                          >  1:473299/1‑100 (MQ=255)
                                      tGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCt                                                         >  1:2296007/1‑100 (MQ=255)
                                       ggATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTa                                                        >  1:3929578/1‑100 (MQ=255)
                                          ttCCAGGCCCGGATTGCAATACGCCATCCGGGCGCGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGa                                                     >  1:1926206/1‑100 (MQ=255)
                                           tCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGAt                                                    <  2:1978491/100‑1 (MQ=255)
                                            ccAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATc                                                   >  2:3153946/1‑100 (MQ=255)
                                             cAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATcc                                                  >  1:1859859/1‑100 (MQ=255)
                                               ggCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATccgc                                                >  2:3326651/1‑100 (MQ=255)
                                                gCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATccgcc                                               >  1:1344050/1‑100 (MQ=255)
                                                gCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATccgcc                                               <  2:3925813/100‑1 (MQ=255)
                                                 cccGGATTGCAATACGCCATCCGGGCAGGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGCAGTAGCACGCCAGGGCGCTATGATccgccg                                              >  2:1599654/1‑100 (MQ=255)
                                                  ccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGt                                             <  2:998130/100‑1 (MQ=255)
                                                   cGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGtt                                            >  1:3140075/1‑100 (MQ=255)
                                                     gATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTcc                                          <  2:2271199/100‑1 (MQ=255)
                                                     gATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTcc                                          <  2:2203530/100‑1 (MQ=255)
                                                     gATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTcc                                          <  2:2964881/100‑1 (MQ=255)
                                                     gATGGCAAGACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTcc                                          <  2:3188630/100‑1 (MQ=255)
                                                        tGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTaa                                       >  1:194729/1‑100 (MQ=255)
                                                          cAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAAc                                     <  2:665528/100‑1 (MQ=255)
                                                          cAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAAc                                     >  2:2370150/1‑100 (MQ=255)
                                                          cAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAAc                                     <  1:3055282/100‑1 (MQ=255)
                                                          cAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAAc                                     >  1:2465556/1‑100 (MQ=255)
                                                            aTACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCa                                   >  2:3400475/1‑100 (MQ=255)
                                                            aTACGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCa                                   <  2:2800940/100‑1 (MQ=255)
                                                              aCGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATc                                 <  1:218132/100‑1 (MQ=255)
                                                               cGCCATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCt                                <  2:1786228/100‑1 (MQ=255)
                                                                 ccATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGc                              >  1:3703393/1‑100 (MQ=255)
                                                                 ccATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGc                              <  2:1757704/100‑1 (MQ=255)
                                                                 ccATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGc                              >  1:1652666/1‑100 (MQ=255)
                                                                  cATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCt                             <  1:1773398/100‑1 (MQ=255)
                                                                  cATCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCt                             <  1:2943339/100‑1 (MQ=255)
                                                                  cATCAGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCt                             >  2:3774970/1‑100 (MQ=255)
                                                                    tCCGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCg                           >  2:2640838/1‑100 (MQ=255)
                                                                     ccGGGCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGc                          <  1:1441695/100‑1 (MQ=255)
                                                                        ggCACGACGTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGt                       <  2:3048378/100‑1 (MQ=255)
                                                                           acgacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                    >  1:704370/1‑100 (MQ=255)
                                                                           acgacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                    <  1:2298928/100‑1 (MQ=255)
                                                                           acgacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                    <  1:736721/100‑1 (MQ=255)
                                                                            cgacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGCTCCGCCGTTCCTAAACCATCCCCTCGCAGTGCCt                   >  1:515199/1‑100 (MQ=255)
                                                                            cgacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                   >  2:195637/1‑100 (MQ=255)
                                                                             gacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTg                  <  2:916459/100‑1 (MQ=255)
                                                                             gacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTg                  >  2:210555/1‑100 (MQ=255)
                                                                             gacgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTg                  >  1:689110/1‑100 (MQ=255)
                                                                              acgTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGc                 <  2:1565663/100‑1 (MQ=255)
                                                                                gTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcat               <  1:1591400/100‑1 (MQ=255)
                                                                                gTCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCCTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTAccgtgct               >  2:3512624/1‑95 (MQ=255)
                                                                                 tCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc              <  1:2981343/100‑1 (MQ=255)
                                                                                 tCATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc              <  1:1813928/100‑1 (MQ=255)
                                                                                  cATTAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTAGGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatca             <  1:3468226/100‑1 (MQ=255)
                                                                                    ttAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCatcatc           >  1:2349984/1‑100 (MQ=255)
                                                                                    ttAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCatcatc           <  2:2675918/100‑1 (MQ=255)
                                                                                     tAACGAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCAtct          <  1:805035/100‑1 (MQ=255)
                                                                                         gAGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAACCCATCTGCTCGCAGTGCCTGCATCATCTCCAt      <  2:1389513/100‑1 (MQ=255)
                                                                                          aGTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATa     <  2:6323/100‑1 (MQ=255)
                                                                                           gTAACTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATaa    <  2:174734/100‑1 (MQ=255)
                                                                                             aaCTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATaaga  >  1:275593/1‑98 (MQ=255)
                                                                                             aaCTGCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAAcc  <  2:24164/100‑1 (MQ=255)
                                                                                                 |                                                                                               
TACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACC  >  NC_000913/3271783‑3271975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A