Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,461,243	A→C	E74A (GAA→GCA)	gspG →	pseudopilin, cryptic, general secretion pathway

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,461,243	0	A	C	98.6%	504.3 / ‑1.8	143	E74A (GAA→GCA)	gspG	pseudopilin, cryptic, general secretion pathway
Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (70/71); total (70/73)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 143 total aligned reads displayed.

AAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATG  >  NC_000913/3461146‑3461338

aaGCAAAAAGCCGTCCGCGATATTGTGGCGCTGGAACACGCCCTTGATATGTACAAATTCGACAATCATCACTATCCCACCACAAATCAGGGGCTTGCAt                                                                                               <  2:1179876/100‑1 (MQ=255)

 aacAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATc                                                                                              <  2:2648922/98‑1 (MQ=255)

 aGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATc                                                                                              >  1:2779908/1‑100 (MQ=255)

  gCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATcc                                                                                             <  1:2475750/100‑1 (MQ=255)

   cAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCt                                                                                            <  2:958472/100‑1 (MQ=255)

    aaaaaGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCtt                                                                                           >  1:3073781/1‑100 (MQ=255)

     aaaaGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTta                                                                                          <  1:604502/100‑1 (MQ=255)

     aaaaGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTta                                                                                          >  1:1843348/1‑100 (MQ=255)

      aaaGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTtag                                                                                         <  2:3938063/100‑1 (MQ=255)

       aaGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTtagt                                                                                        <  1:1817788/100‑1 (MQ=255)

       aaGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTtagt                                                                                        <  2:3954252/100‑1 (MQ=255)

       aaGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAAATCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTtagt                                                                                        <  1:3883417/100‑1 (MQ=255)

        aGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACACATCAGGGGCTTGCATCCTtagta                                                                                       <  2:546841/100‑1 (MQ=255)

         gCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTtagtag                                                                                      >  1:3452920/1‑100 (MQ=255)

           cGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGaa                                                                                    >  1:1910412/1‑100 (MQ=255)

            gTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAg                                                                                   <  1:417660/100‑1 (MQ=255)

            gTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAg                                                                                   >  2:2879512/1‑100 (MQ=255)

            gTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAg                                                                                   <  1:2847922/100‑1 (MQ=255)

            gTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAg                                                                                   >  1:382384/1‑100 (MQ=255)

            gTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAg                                                                                   <  1:624639/100‑1 (MQ=255)

               aGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCGTAGTAGAAgcgc                                                                                >  2:1969176/1‑100 (MQ=255)

                 cGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCg                                                                              >  1:1743320/1‑100 (MQ=255)

                   atatTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGac                                                                            <  1:312164/100‑1 (MQ=255)

                      ttGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACt                                                                         <  1:359756/100‑1 (MQ=255)

                       tgtgGCGCTGGAAAACGCCCTTGATCTGTACACACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCGCTAGTAGAAGCGCCGACAATg                                                                        >  2:3334312/1‑100 (MQ=255)

                       tgtgGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTg                                                                        <  2:3105207/100‑1 (MQ=255)

                         tgGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGcc                                                                      <  1:544061/100‑1 (MQ=255)

                         tgGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGcc                                                                      <  2:378052/100‑1 (MQ=255)

                          ggCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCa                                                                     <  1:4004429/100‑1 (MQ=255)

                           gcgcTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCAc                                                                    <  2:527038/100‑1 (MQ=255)

                            cgcTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCAcc                                                                   >  2:1717611/1‑100 (MQ=255)

                            cgcTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCAcc                                                                   >  1:2066267/1‑100 (MQ=255)

                                ggAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTg                                                               <  1:604996/100‑1 (MQ=255)

                                  aaaaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGc                                                             >  1:244013/1‑100 (MQ=255)

                                  aaaaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGc                                                             <  1:3615104/100‑1 (MQ=255)

                                   aaaCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGcc                                                            <  2:429879/100‑1 (MQ=255)

                                    aaCGCCCTTGATATGTACAAACTCGACAAGCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCg                                                           <  1:1007070/100‑1 (MQ=255)

                                    aaCGCCCTTGATATGCACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCg                                                           <  2:43183/100‑1 (MQ=255)

                                     aCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCga                                                          >  2:1921227/1‑99 (MQ=255)

                                     aCGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGc                                                          >  1:2770055/1‑100 (MQ=255)

                                       gCCCTTGATATGTACAAACTCGACAATCATAACTATCCAACCACAAATCAGGGGCTTGCATCCCTAGTAGAGGCGCCGACACTGCCACCGCTGGCCGCaa                                                        >  2:3699470/1‑100 (MQ=255)

                                        cccTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCaca                                                       >  2:2924462/1‑98 (MQ=255)

                                          cTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACt                                                     <  1:1000741/100‑1 (MQ=255)

                                          cTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACt                                                     >  2:1190032/1‑100 (MQ=255)

                                          cTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACt                                                     <  1:726936/100‑1 (MQ=255)

                                             gATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACtata                                                  <  1:155593/100‑1 (MQ=255)

                                             gATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACtata                                                  <  1:1757245/100‑1 (MQ=255)

                                             gATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACtata                                                  <  2:2976194/100‑1 (MQ=255)

                                               tatGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAAc                                                >  2:96131/1‑100 (MQ=255)

                                               tatGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAAc                                                <  1:37276/100‑1 (MQ=255)

                                               tatGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAAc                                                <  1:37419/100‑1 (MQ=255)

                                                  gTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGTAAACTATAACaag                                             >  1:3670722/1‑100 (MQ=255)

                                                  gTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaag                                             <  2:2276710/100‑1 (MQ=255)

                                                  gTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaag                                             >  2:674493/1‑100 (MQ=255)

                                                      aaaCTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACaaggaag                                         >  1:2215445/1‑100 (MQ=255)

                                                        aCTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGt                                       >  2:1341065/1‑100 (MQ=255)

                                                         cTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGtt                                      >  1:4051699/1‑100 (MQ=255)

                                                         cTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGtt                                      >  1:2242488/1‑100 (MQ=255)

                                                           cGACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTtat                                    >  2:2751935/1‑100 (MQ=255)

                                                            gACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAGCAAGGAAGGTtata                                   <  2:3365401/100‑1 (MQ=255)

                                                            gACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTtata                                   >  2:1757602/1‑100 (MQ=255)

                                                            gACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTtata                                   <  1:1349923/100‑1 (MQ=255)

                                                            gACAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTtata                                   >  2:321572/1‑100 (MQ=255)

                                                              cAATCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATc                                 <  1:3120715/100‑1 (MQ=255)

                                                               aaTCATCACTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCa                                >  1:254347/1‑100 (MQ=255)

                                                                 tcatcaCTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAg                              <  1:1082818/100‑1 (MQ=255)

                                                                 tcatcaCTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAg                              <  1:2247227/100‑1 (MQ=255)

                                                                 tcatcaCTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAg                              <  1:3902058/100‑1 (MQ=255)

                                                                  catcaCTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGc                             >  1:2629598/1‑100 (MQ=255)

                                                                   atcaCTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCg                            >  2:916223/1‑100 (MQ=255)

                                                                     caCTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTc                          >  1:3318153/1‑100 (MQ=255)

                                                                       cTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctg                        <  1:1392908/100‑1 (MQ=255)

                                                                       cTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctg                        >  2:2722469/1‑100 (MQ=255)

                                                                       cTATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctg                        <  1:1256292/100‑1 (MQ=255)

                                                                        tATCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgc                       >  1:754094/1‑100 (MQ=255)

                                                                         ctCCAACCACAAGTCAGTGGCTTGCATCCTTAGTAGAAGCGCCGACACTGACACCGCTGGCCGCAAACTATAACATGGAAGGTTATATCAAGCGTctgcc                      <  2:204430/99‑1 (MQ=255)

                                                                         aTCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgcc                      <  1:327414/100‑1 (MQ=255)

                                                                         aTCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgcc                      >  2:1232292/1‑100 (MQ=255)

                                                                          tCCAACCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTctgcct                     >  2:2760140/1‑100 (MQ=255)

                                                                            cAACCGCAAATCAGGGGCTTGCATCATTAGTAGAAGCGCCGACACTGCCACCGCTCGCCGCAAACTATCACAAGGAAGGTCATATAAAGCGTctccctgc                   >  2:3519784/1‑100 (MQ=255)

                                                                             aaCCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCtgcctgcc                  >  1:428967/1‑100 (MQ=255)

                                                                              aCCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCg                 >  1:294831/1‑100 (MQ=255)

                                                                              aCCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCg                 >  1:2126704/1‑100 (MQ=255)

                                                                              aCCACAAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCg                 <  1:3023454/100‑1 (MQ=255)

                                                                                cacaAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGAt               >  2:1768657/1‑100 (MQ=255)

                                                                                cacaAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGAt               <  2:2241854/100‑1 (MQ=255)

                                                                                 acaAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATc              >  1:2582922/1‑100 (MQ=255)

                                                                                  caAATCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATcc             >  1:369088/1‑100 (MQ=255)

                                                                                   aaaTCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATccc            <  1:92697/100‑1 (MQ=255)

                                                                                    aaTCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCt           <  2:2452009/100‑1 (MQ=255)

                                                                                    aaTCAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCt           >  2:3512417/1‑100 (MQ=255)

                                                                                       cAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTggn        <  1:611151/100‑2 (MQ=255)

                                                                                       cAGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTggg        <  1:1797770/100‑1 (MQ=255)

                                                                                        aGGGGCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTgggg       <  1:1745526/100‑1 (MQ=255)

                                                                                         ggggCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGc      >  2:400355/1‑100 (MQ=255)

                                                                                         ggggCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGc      <  2:1133839/100‑1 (MQ=255)

                                                                                         ggggCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGc      >  2:1080721/1‑100 (MQ=255)

                                                                                            gCTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAAt   <  2:290016/100‑1 (MQ=255)

                                                                                             cTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATg  <  2:2093838/100‑1 (MQ=255)

                                                                                             cTTGCATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATg  <  1:2980595/100‑1 (MQ=255)

AAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATG  >  NC_000913/3461146‑3461338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A