Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,468,138 T→G G572G (GGA→GGC)  chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,468,1380TG98.5% 452.7 / ‑1.7 137G572G (GGA→GGC) chiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (70/65);  total (71/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01

Only 100 of 137 total aligned reads displayed.

CAGCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTTCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCGA  >  NC_000913/3468041‑3468234
                                                                                                 |                                                                                                
cAGCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGcc                                                                                                >  1:3089425/1‑100 (MQ=255)
cAGCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGcc                                                                                                <  2:1366856/100‑1 (MQ=255)
 aGCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCg                                                                                               <  1:1625636/100‑1 (MQ=255)
 aGCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCg                                                                                               >  2:271676/1‑100 (MQ=255)
  gCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCgg                                                                                              <  2:755421/100‑1 (MQ=255)
  gCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCgg                                                                                              <  2:2923769/100‑1 (MQ=255)
   ccAGATCCGGGATCGTATTCAGCGTCAAGTCGACATACGGTGCATAAACGTGTTTCGACCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCgct                                                                                             >  2:2810510/1‑98 (MQ=255)
   ccAGATCCGGGATCGTATTCAGCGTAAAGTCGACATATGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACTTACGCGCTCTTTGGTGCCGGt                                                                                             <  2:3634216/100‑1 (MQ=255)
    cAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTc                                                                                            >  2:2738556/1‑100 (MQ=255)
    cAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTc                                                                                            <  2:1455339/100‑1 (MQ=255)
    cAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTc                                                                                            >  2:550025/1‑100 (MQ=255)
     aGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGTCGGTcc                                                                                           <  1:81515/100‑1 (MQ=255)
     aGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTcc                                                                                           >  2:3691725/1‑100 (MQ=255)
     aGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTcc                                                                                           >  2:1930822/1‑100 (MQ=255)
     aGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTcc                                                                                           <  1:1930608/100‑1 (MQ=255)
     aGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTATTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTcc                                                                                           <  2:3933237/100‑1 (MQ=255)
      gATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCa                                                                                          >  1:1051019/1‑100 (MQ=255)
      gATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCa                                                                                          <  1:2477587/100‑1 (MQ=255)
         ccGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTc                                                                                       <  1:3369098/100‑1 (MQ=255)
         ccGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTc                                                                                       <  1:2664227/100‑1 (MQ=255)
         ccGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTc                                                                                       >  2:316070/1‑100 (MQ=255)
          cGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTca                                                                                      >  1:2066984/1‑100 (MQ=255)
           gggATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTcaa                                                                                     >  1:1944146/1‑100 (MQ=255)
            gcaTCGTATTCAGCGTAAAGTCGACATACCGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTcaac                                                                                    <  2:2406670/98‑1 (MQ=255)
               tCGTATTCTGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACaaa                                                                                 <  2:1890578/100‑1 (MQ=255)
                 gTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACaaaaa                                                                               >  1:1342425/1‑100 (MQ=255)
                 gTATTCAGCGTAAAGTCCACATACGGTGCATAAACGTGTTTCGGTCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACaaaaa                                                                               <  1:3753173/100‑1 (MQ=255)
                  tATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAac                                                                              <  1:561352/100‑1 (MQ=255)
                       aGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGcc                                                                         <  1:2975486/100‑1 (MQ=255)
                         cGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCAc                                                                       >  1:3967007/1‑100 (MQ=255)
                            aaaGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACggg                                                                    <  1:1835950/100‑1 (MQ=255)
                             aaGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGt                                                                   <  1:1323348/100‑1 (MQ=255)
                              aGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGtt                                                                  <  1:2192878/100‑1 (MQ=255)
                               gTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTg                                                                 >  1:710483/1‑100 (MQ=255)
                               gTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTg                                                                 >  1:1568137/1‑100 (MQ=255)
                                tCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGc                                                                <  1:3386521/100‑1 (MQ=255)
                                  gACATACGGTGCATAAACGTGTTTCGGCCTCGCTTTCTTCGGCGTACCTTCGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTg                                                              <  2:46240/100‑1 (MQ=255)
                                  gACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTg                                                              >  1:2857616/1‑100 (MQ=255)
                                  gACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTg                                                              <  1:2322396/100‑1 (MQ=255)
                                   aCATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGt                                                             >  1:5627/1‑100 (MQ=255)
                                    cATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTc                                                            <  1:1099348/100‑1 (MQ=255)
                                    cATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTc                                                            <  1:1437080/100‑1 (MQ=255)
                                     aTACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTCGTGCCGGTCCAGTCCACAACCACACGCCACGGGTTGCTGTCa                                                           >  2:3266052/1‑100 (MQ=255)
                                      tACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAg                                                          <  2:2294366/100‑1 (MQ=255)
                                      tACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAg                                                          >  1:4002050/1‑100 (MQ=255)
                                       aCGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGa                                                         <  1:1760670/100‑1 (MQ=255)
                                         ggTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAg                                                       >  1:888380/1‑100 (MQ=255)
                                         ggTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAg                                                       >  2:1685657/1‑100 (MQ=255)
                                          gTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAgg                                                      >  1:2625666/1‑100 (MQ=255)
                                            gCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGgag                                                    >  1:766092/1‑100 (MQ=255)
                                            gCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGgag                                                    >  1:2449758/1‑100 (MQ=255)
                                             cATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGgaga                                                   >  1:2810908/1‑100 (MQ=255)
                                                aaaCGGGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAAc                                                >  2:594977/1‑100 (MQ=255)
                                                   cGTGTTTCGGCCACGCTTTCTTCGGCGAAAATACGCCCTCTTTGGTGCCGGTACAGTCAATAAAAAAACGCCCCGGGTTCAAGTCAGCAGGAGAACCttt                                             >  2:2401716/1‑100 (MQ=255)
                                                    gtgtTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTc                                            <  2:2027579/100‑1 (MQ=255)
                                                       tttCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGc                                         <  2:1541470/100‑1 (MQ=255)
                                                          cGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACt                                      >  2:1608724/1‑100 (MQ=255)
                                                           ggCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACtt                                     >  2:1701455/1‑100 (MQ=255)
                                                           ggCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACtt                                     >  1:1759065/1‑100 (MQ=255)
                                                            gCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACttt                                    >  2:3055452/1‑100 (MQ=255)
                                                            gCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACttt                                    >  1:2510981/1‑100 (MQ=255)
                                                             ccACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTa                                   >  1:2608615/1‑100 (MQ=255)
                                                             ccACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTa                                   >  1:340927/1‑100 (MQ=255)
                                                              cACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTAc                                  >  1:1602789/1‑100 (MQ=255)
                                                              cACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTAc                                  <  2:1105363/100‑1 (MQ=255)
                                                                cGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTActc                                >  1:2686722/1‑100 (MQ=255)
                                                                 gCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTActct                               >  2:454825/1‑100 (MQ=255)
                                                                  cTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTg                              >  2:1493801/1‑100 (MQ=255)
                                                                    ttcttcGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTgag                            >  2:571473/1‑100 (MQ=255)
                                                                    ttcttcGGCGTACCTACGCGCTCTTTGGTGCCGGGCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTgag                            <  2:68823/100‑1 (MQ=255)
                                                                     tcttcGGCGGACCTGCGCGGTCTTTGTTGCCGGAGCAATCAATAAAATCGCGCTACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTgaga                           <  2:1350546/100‑1 (MQ=255)
                                                                      cttcGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGaa                          >  2:2165321/1‑100 (MQ=255)
                                                                       ttcGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGaaa                         <  1:2899728/100‑1 (MQ=255)
                                                                         cGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATg                       <  1:1605868/100‑1 (MQ=255)
                                                                          ggCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGt                      >  2:3162411/1‑100 (MQ=255)
                                                                          ggCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGt                      >  1:57485/1‑100 (MQ=255)
                                                                          ggCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGt                      >  1:3604358/1‑100 (MQ=255)
                                                                           gCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGGTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTa                     >  1:3501210/1‑100 (MQ=255)
                                                                            cGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAg                    <  1:1730353/100‑1 (MQ=255)
                                                                            cGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAg                    <  1:2638229/100‑1 (MQ=255)
                                                                             gTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGt                   <  1:1120039/100‑1 (MQ=255)
                                                                             gTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGt                   <  1:1014689/100‑1 (MQ=255)
                                                                                 cTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTcac               <  2:1048556/100‑1 (MQ=255)
                                                                                  tACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTcaca              >  2:3709054/1‑100 (MQ=255)
                                                                                   aCGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCacac             >  2:2482647/1‑100 (MQ=255)
                                                                                   aCGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCacac             >  1:1131260/1‑100 (MQ=255)
                                                                                    cgcgCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACAcc            <  1:276237/100‑1 (MQ=255)
                                                                                    cgcgCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACAcc            >  1:2426391/1‑100 (MQ=255)
                                                                                    cgcgCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACAcc            >  1:1080451/1‑100 (MQ=255)
                                                                                    cgcgCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACAcc            >  1:375925/1‑100 (MQ=255)
                                                                                     gcgcTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCg           >  2:3480800/1‑100 (MQ=255)
                                                                                      cgcTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGt          <  2:3491073/100‑1 (MQ=255)
                                                                                         tctTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTaa       <  1:1567864/100‑1 (MQ=255)
                                                                                          ctTTGGTTCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTaaa      >  1:3883047/1‑100 (MQ=255)
                                                                                           tttGGTGCCAGACGAGTAAACACAAACATGCCAGGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTCCTCTGAGAAATGTAGTTCACACCGTTAAAg     <  2:108373/100‑1 (MQ=255)
                                                                                            ttGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGc    >  1:665860/1‑100 (MQ=255)
                                                                                             tGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCg   >  1:2372865/1‑100 (MQ=255)
                                                                                             tGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCg   <  1:3764541/100‑1 (MQ=255)
                                                                                              ggTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCGa  >  1:1900990/1‑100 (MQ=255)
                                                                                              ggTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCGa  >  1:3193447/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CAGCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTTCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCGA  >  NC_000913/3468041‑3468234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A