Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,679,181 T→G R3R (AGG→CGG)  yhjH ← cyclic‑di‑GMP phosphodiesterase, FlhDC‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,679,1810TG97.4% 529.9 / 0.3 155R3R (AGG→CGG) yhjHcyclic‑di‑GMP phosphodiesterase, FlhDC‑regulated
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (86/64);  total (88/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

GCCAGGTGTAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCTTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCGGGG  >  NC_000913/3679085‑3679276
                                                                                                |                                                                                               
gCCAGGTGTAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTat                                                                                              >  1:2559656/1‑100 (MQ=255)
gCCAGGTGTAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCCCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTat                                                                                              >  1:2346055/1‑100 (MQ=255)
  cAGGTGTAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTatca                                                                                            >  1:2889210/1‑100 (MQ=255)
  cAGGTGTAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTatca                                                                                            <  2:2147937/100‑1 (MQ=255)
   aGGTGTAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTatcat                                                                                           <  1:3367172/100‑1 (MQ=255)
   aGGTGTAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTatcat                                                                                           >  1:3781733/1‑100 (MQ=255)
    ggTGTAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTatcatc                                                                                          >  1:729102/1‑100 (MQ=255)
     gtgtAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTAtcatca                                                                                         <  1:2137734/100‑1 (MQ=255)
       gtAAGCACGCTCACACGGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCaaa                                                                                       <  2:2182464/100‑1 (MQ=255)
        tAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAg                                                                                      >  1:1053160/1‑100 (MQ=255)
        tAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAg                                                                                      >  2:722154/1‑100 (MQ=255)
        tAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAg                                                                                      >  1:4065055/1‑100 (MQ=255)
        tAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAg                                                                                      <  1:408878/100‑1 (MQ=255)
         aaGCACGCTCACACTGCAACTAAAAACGCCGTTCCTGCAAGCTCTCGATGCGTGCTTCATGGTTGCTTATTCGCTGGATAATCTGCCGTATCATCTAAGa                                                                                     >  2:3392961/1‑100 (MQ=255)
         aaGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGa                                                                                     >  1:2738904/1‑100 (MQ=255)
          aGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGAt                                                                                    >  2:1519292/1‑100 (MQ=255)
          aGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGAt                                                                                    >  1:1279877/1‑100 (MQ=255)
           gCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATg                                                                                   >  1:1684341/1‑100 (MQ=255)
            cACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGt                                                                                  <  1:3981428/100‑1 (MQ=255)
              cGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGtac                                                                                >  2:2467379/1‑98 (MQ=255)
              cGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTcc                                                                                >  1:732579/1‑100 (MQ=255)
              cGCTCACACTGCAACCAAAAACGCCGATCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGGTGGATAACCTGCCGTATCATCAAAGATGTcc                                                                                <  2:3806468/100‑1 (MQ=255)
                 tCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCtgt                                                                             <  2:1057457/100‑1 (MQ=255)
                  cacaCTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCtgtg                                                                            >  1:2315907/1‑100 (MQ=255)
                  cacaCTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCtgtg                                                                            >  1:2470780/1‑100 (MQ=255)
                     acTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGttt                                                                         <  1:2277171/100‑1 (MQ=255)
                     acTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGttt                                                                         >  1:2580196/1‑100 (MQ=255)
                      cTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTa                                                                        >  1:991484/1‑100 (MQ=255)
                      cTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTa                                                                        <  2:1037149/100‑1 (MQ=255)
                      cTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTa                                                                        >  1:1076325/1‑100 (MQ=255)
                        gCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTaaa                                                                      <  1:3487326/100‑1 (MQ=255)
                        gCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTaaa                                                                      <  1:1711201/100‑1 (MQ=255)
                          aaCCAAAAACGCCGTTCCTGCAAGCTCTCGATTCTTGCTTCAGGATTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAg                                                                    <  1:3742039/100‑1 (MQ=255)
                          aaCCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCTCTTGATAATTTTTTTTTTCATCAAAGATGTCCTGTGTTTAAAAg                                                                    >  2:516689/1‑100 (MQ=255)
                          aaCCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAg                                                                    <  2:1814193/100‑1 (MQ=255)
                           aCCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGt                                                                   >  2:3240243/1‑100 (MQ=255)
                           aCCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGt                                                                   >  1:608232/1‑100 (MQ=255)
                            ccAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCTTATCATCAAAGATGTCCTGTGTTTAAAAGTg                                                                  >  2:3030640/1‑100 (MQ=255)
                            ccAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTg                                                                  >  1:3148943/1‑100 (MQ=255)
                            ccAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTg                                                                  <  2:1519961/100‑1 (MQ=255)
                            ccAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTg                                                                  <  1:84768/100‑1 (MQ=255)
                             cAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGa                                                                 >  1:1821817/1‑100 (MQ=255)
                              aaaaaCGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGAt                                                                <  2:2279274/100‑1 (MQ=255)
                                 aaCGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGAtgct                                                             <  2:3556025/100‑1 (MQ=255)
                                     ccGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGccc                                                         >  1:3390267/1‑100 (MQ=255)
                                      cGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCg                                                        <  2:2212051/100‑1 (MQ=255)
                                       gTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCgg                                                       >  1:1655920/1‑100 (MQ=255)
                                       gTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCgg                                                       >  2:49962/1‑100 (MQ=255)
                                           cTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTc                                                   >  1:1462482/1‑100 (MQ=255)
                                            tGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCg                                                  >  1:1280849/1‑100 (MQ=255)
                                              cAAGCGCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCATATCATCGAAGATGTCCTGTGTTTAAAAGTGATGCGGCCCGGACTCGAc                                                <  1:3870061/100‑1 (MQ=255)
                                               aaGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACa                                               <  2:1345767/100‑1 (MQ=255)
                                               aaGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACa                                               >  1:891783/1‑100 (MQ=255)
                                                 gCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACaaa                                             >  1:743124/1‑100 (MQ=255)
                                                  ctctCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAg                                            >  2:3496635/1‑100 (MQ=255)
                                                       gATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTAt                                       >  2:3502490/1‑100 (MQ=255)
                                                         tgcttgctTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCg                                     <  1:3775041/100‑1 (MQ=255)
                                                         tgcttgctTCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCg                                     >  1:3688637/1‑100 (MQ=255)
                                                          gcttgcttCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCgg                                    >  1:3039255/1‑100 (MQ=255)
                                                           cttgcttCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGgc                                   >  2:2419071/1‑100 (MQ=255)
                                                           cttgcttCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGgc                                   <  2:2490743/100‑1 (MQ=255)
                                                             tgcttCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGgcgc                                 >  2:1894758/1‑100 (MQ=255)
                                                              gcttCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGcgcg                                <  2:2717972/100‑1 (MQ=255)
                                                              gcttCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGcgcg                                <  2:3406945/100‑1 (MQ=255)
                                                              gcttCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGcgcg                                >  2:3668272/1‑100 (MQ=255)
                                                                 tCAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTaa                             <  1:55242/100‑1 (MQ=255)
                                                                  cAGGGTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAg                            <  2:3563576/100‑1 (MQ=255)
                                                                     ggTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGc                         <  1:770378/100‑1 (MQ=255)
                                                                     ggTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGc                         <  1:2982911/100‑1 (MQ=255)
                                                                     ggTTGCTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGc                         <  2:2508322/100‑1 (MQ=255)
                                                                          cTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAAc                    <  1:2679573/100‑1 (MQ=255)
                                                                          cTTATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAAc                    >  1:3834005/1‑100 (MQ=255)
                                                                           ttATTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACt                   <  1:1439813/100‑1 (MQ=255)
                                                                             aTTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGTTGCCGCCCGGACTCGACAAAGATTATCGGCGTGTAATGGCAGAACttt                 <  1:3580023/100‑1 (MQ=255)
                                                                             aTTCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACttt                 <  1:527024/100‑1 (MQ=255)
                                                                              ttCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTa                <  1:1925425/100‑1 (MQ=255)
                                                                               tCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGCTTATCGGCGCGTAAGGGCAGAACTTTAc               >  1:1614065/1‑100 (MQ=255)
                                                                               tCGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTAc               <  2:3835534/100‑1 (MQ=255)
                                                                                cGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTAcc              >  2:840246/1‑100 (MQ=255)
                                                                                cGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTAcc              <  2:3718112/100‑1 (MQ=255)
                                                                                cGCTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTAcc              >  2:1758946/1‑100 (MQ=255)
                                                                                 gCTGGATAACCTGCCTTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCg             <  1:159799/100‑1 (MQ=255)
                                                                                  cTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGc            >  2:1259365/1‑100 (MQ=255)
                                                                                  cTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGc            >  1:3914638/1‑100 (MQ=255)
                                                                                  cTGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGc            >  1:1376546/1‑100 (MQ=255)
                                                                                   tGGATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGcc           <  1:1054510/100‑1 (MQ=255)
                                                                                    ggATACCCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCt          <  2:448933/100‑1 (MQ=255)
                                                                                    ggATAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCt          >  1:322895/1‑100 (MQ=255)
                                                                                       tacccTGCCGTCTTATCAAAGATGGCCTGTGTTTAAAAGTGATGCTGCCCGGACTTTACAAAGATTATTTTCGCGTTAGGGAAGAACTTTATCGCTTGGc       >  2:792117/4‑100 (MQ=255)
                                                                                       tAACCTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGc       >  1:1080320/1‑100 (MQ=255)
                                                                                          ccTGCCGTATCATCAAAGCTGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAATGGCAGAACTTTACCGCCTGGCCgg    >  2:2904993/1‑100 (MQ=255)
                                                                                          ccTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCgg    >  1:2774788/1‑100 (MQ=255)
                                                                                          ccTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCgg    >  2:2977311/1‑100 (MQ=255)
                                                                                           cTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCggg   <  1:206449/100‑1 (MQ=255)
                                                                                           cTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCggg   >  1:217788/1‑100 (MQ=255)
                                                                                           cTGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCggg   <  1:1416249/100‑1 (MQ=255)
                                                                                            tGCCTTATCATCAAAGATGTCCTGTGTTTAACAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCgggg  <  1:3076360/100‑1 (MQ=255)
                                                                                            tGCCGTATCATCAAAGGTGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCgggg  <  2:1663015/100‑1 (MQ=255)
                                                                                            tGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCgggg  <  1:389642/100‑1 (MQ=255)
                                                                                            tGCCGTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCgggg  <  2:2280534/100‑1 (MQ=255)
                                                                                                |                                                                                               
GCCAGGTGTAAGCACGCTCACACTGCAACCAAAAACGCCGTTCCTGCAAGCTCTCGATGCTTGCTTCAGGGTTGCTTATTCGCTGGATAACCTGCCTTATCATCAAAGATGTCCTGTGTTTAAAAGTGATGCTGCCCGGACTCGACAAAGATTATCGGCGCGTAAGGGCAGAACTTTACCGCCTGGCCGGGG  >  NC_000913/3679085‑3679276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A