Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,716,242	A→C	V51V (GTT→GTG)	bisC ←	biotin sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,716,242	0	A	C	97.3%	499.0 / ‑2.0	147	V51V (GTT→GTG)	bisC	biotin sulfoxide reductase
Reads supporting (aligned to +/- strand): ref base A (2/2); new base C (64/79); total (66/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

GAACAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGAACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCAGC  >  NC_000913/3716145‑3716338

gAACAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCACACCATTGGACATCATACCCGCGTATTGCTGTGCAc                                                                                                >  2:3788735/1‑100 (MQ=255)

gAACAAACTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCAc                                                                                                <  2:806068/100‑1 (MQ=255)

 aaCAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCAcc                                                                                               >  2:3224264/1‑100 (MQ=255)

 aaCAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCAcc                                                                                               <  1:2999267/100‑1 (MQ=255)

 aaCAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCAcc                                                                                               <  1:2853362/100‑1 (MQ=255)

   cAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTg                                                                                             >  2:919259/1‑100 (MQ=255)

   cAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTg                                                                                             >  2:909764/1‑100 (MQ=255)

    aaaTTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTgg                                                                                            <  2:3379984/100‑1 (MQ=255)

    aaaTTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGAACCTgg                                                                                            <  1:1172938/100‑1 (MQ=255)

     aaTTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCCTTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGt                                                                                           >  2:281733/1‑100 (MQ=255)

      aTTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTc                                                                                          <  1:2442708/100‑1 (MQ=255)

      aTTCATCCTGCCCACCAATACCCTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAACCGTCCCCGCGTATTGCTGTGCACCTGGTc                                                                                          <  1:2441035/100‑1 (MQ=255)

          aTCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCCAGAAAGACTTTTCGCACCATTGGAAATCGTACAAGAGTATTGCTGTGCACCTGGTCGCGa                                                                                      >  2:384599/1‑100 (MQ=255)

            ccTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAAc                                                                                    <  1:3372979/100‑1 (MQ=255)

              tGCCTACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGAACCTGGTCGCGAACcg                                                                                  <  2:1761840/100‑1 (MQ=255)

              tGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACcg                                                                                  >  2:3976920/1‑100 (MQ=255)

                cccACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACcgcg                                                                                <  1:1723978/100‑1 (MQ=255)

                 ccACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAACTCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCgcgc                                                                               <  2:1158320/100‑1 (MQ=255)

                 ccACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCgcgc                                                                               >  1:1915389/1‑100 (MQ=255)

                  cACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGct                                                                              >  1:3699398/1‑100 (MQ=255)

                    cGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGctct                                                                            >  1:3791028/1‑100 (MQ=255)

                    cGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGctct                                                                            >  1:2867239/1‑100 (MQ=255)

                     gAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTg                                                                           >  1:1063260/1‑100 (MQ=255)

                         gCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAg                                                                       >  1:1429415/1‑100 (MQ=255)

                           cTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGa                                                                     >  2:2769990/1‑100 (MQ=255)

                           cTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGa                                                                     >  1:3743201/1‑100 (MQ=255)

                             tGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGt                                                                   <  2:2397629/100‑1 (MQ=255)

                                gggTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGtttt                                                                >  1:4034352/1‑100 (MQ=255)

                                gggTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGtttt                                                                <  2:2170922/100‑1 (MQ=255)

                                 ggTTTTCCGGTGACGCGAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTc                                                               <  1:3763053/100‑1 (MQ=255)

                                 ggTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTc                                                               >  2:1429603/1‑100 (MQ=255)

                                  gTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTcc                                                              <  1:1381180/100‑1 (MQ=255)

                                  gTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTcc                                                              >  2:1885148/1‑100 (MQ=255)

                                  gTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTcc                                                              >  1:2876042/1‑100 (MQ=255)

                                  gTTTTCCGCTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTcc                                                              <  2:823754/100‑1 (MQ=255)

                                    tttCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCAt                                                            <  1:4050568/100‑1 (MQ=255)

                                       ccGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTcc                                                         <  2:58431/100‑1 (MQ=255)

                                       ccGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTcc                                                         <  2:3045465/100‑1 (MQ=255)

                                       ccGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTcc                                                         <  1:1116963/100‑1 (MQ=255)

                                        cGGTGGCGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCt                                                        >  2:3515243/1‑100 (MQ=255)

                                        cGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCt                                                        >  2:2977054/1‑100 (MQ=255)

                                        cGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCt                                                        >  2:2185435/1‑100 (MQ=255)

                                          gTGACGCAAGAAAGCCTTTTCTCTTTTTTTTAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCtgt                                                      >  2:610866/1‑100 (MQ=255)

                                            gACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTgg                                                    <  1:664486/100‑1 (MQ=255)

                                            gACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTgg                                                    >  2:152354/1‑100 (MQ=255)

                                            gACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCGGTgg                                                    <  2:3405195/100‑1 (MQ=255)

                                              cGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCt                                                  >  1:467044/1‑100 (MQ=255)

                                              cGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCt                                                  <  2:223556/100‑1 (MQ=255)

                                                cAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTaa                                                >  2:2978997/1‑100 (MQ=255)

                                                 aaGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAAc                                               <  1:2171451/100‑1 (MQ=255)

                                                  aGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAAcg                                              >  1:2472887/1‑100 (MQ=255)

                                                  aGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAAcg                                              <  2:1500455/100‑1 (MQ=255)

                                                        ccTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCAc                                        >  1:2331892/1‑100 (MQ=255)

                                                         cTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCAcg                                       <  1:3573607/100‑1 (MQ=255)

                                                           tttCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCAcgcg                                     >  1:1691214/1‑100 (MQ=255)

                                                              tctaCCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGc                                  <  1:3205984/97‑1 (MQ=255)

                                                              cGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGc                                  >  1:3449679/1‑100 (MQ=255)

                                                              cGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGc                                  <  1:819791/100‑1 (MQ=255)

                                                                 aCCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGcaga                               >  2:3156819/1‑97 (MQ=255)

                                                                  ccATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTaaa                              >  2:3766868/1‑100 (MQ=255)

                                                                  ccATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTaaa                              <  1:1358890/100‑1 (MQ=255)

                                                                   cATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAac                             >  2:2330615/1‑100 (MQ=255)

                                                                    aTTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGTCTAACGCGCCACGCGAGCTAAtca                            >  1:2878740/1‑100 (MQ=255)

                                                                    aTTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAaca                            >  2:2508385/1‑100 (MQ=255)

                                                                     ttGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAacac                           >  1:928642/1‑100 (MQ=255)

                                                                     ttGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAacac                           >  2:1979542/1‑100 (MQ=255)

                                                                       ggAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACgg                         <  2:685188/100‑1 (MQ=255)

                                                                        gAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGt                        <  1:1083660/100‑1 (MQ=255)

                                                                        gAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGt                        <  2:2578373/100‑1 (MQ=255)

                                                                        gAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGt                        <  1:3869332/100‑1 (MQ=255)

                                                                        gAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGt                        <  2:3071402/100‑1 (MQ=255)

                                                                          aaTCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGttt                      <  1:2322702/100‑1 (MQ=255)

                                                                            tCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCg                    <  2:2305874/100‑1 (MQ=255)

                                                                            tCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCg                    <  1:2271611/100‑1 (MQ=255)

                                                                             cGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGc                   >  1:1406348/1‑100 (MQ=255)

                                                                             cGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGc                   <  1:391709/100‑1 (MQ=255)

                                                                              gTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGcc                  <  2:2316711/100‑1 (MQ=255)

                                                                                 cccGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTc               >  1:1590356/1‑100 (MQ=255)

                                                                                 ccagcgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTc               >  1:361628/4‑100 (MQ=255)

                                                                                  ccGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCg              <  1:426343/100‑1 (MQ=255)

                                                                                  ccGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCGCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCg              <  2:2643580/100‑1 (MQ=255)

                                                                                   cgcgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCgg             >  1:4071977/1‑100 (MQ=255)

                                                                                   cgcgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCgg             <  1:1915681/100‑1 (MQ=255)

                                                                                   cgcgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCgg             <  2:566313/100‑1 (MQ=255)

                                                                                   cgcgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCgg             >  1:3288973/1‑100 (MQ=255)

                                                                                   cgcgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCgg             <  1:333427/100‑1 (MQ=255)

                                                                                   cgcgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCgg             <  2:2920204/100‑1 (MQ=255)

                                                                                     cgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGtt           <  1:2183803/100‑1 (MQ=255)

                                                                                      gTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGttt          >  2:2798202/1‑100 (MQ=255)

                                                                                      gTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGttt          <  2:1025306/100‑1 (MQ=255)

                                                                                          tGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAAc      >  1:3464832/1‑100 (MQ=255)

                                                                                           gCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAAcc     <  1:3023948/100‑1 (MQ=255)

                                                                                           gCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAAcc     <  1:938190/100‑1 (MQ=255)

                                                                                           gCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAAcc     <  2:2294088/100‑1 (MQ=255)

                                                                                           gCTGTGAACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAAcc     >  1:275740/1‑100 (MQ=255)

                                                                                            cTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCa    >  2:2264555/1‑100 (MQ=255)

                                                                                             tgtgCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCAg   >  1:3453706/1‑100 (MQ=255)

                                                                                             tgtgCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCAg   >  2:2963977/1‑100 (MQ=255)

                                                                                              gtgCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCAGc  <  2:3611010/100‑1 (MQ=255)

                                                                                              gtgCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCAGc  <  2:3612218/100‑1 (MQ=255)

GAACAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGAACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCAGC  >  NC_000913/3716145‑3716338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A