Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F236 I1 R1 155 93.2 2393300 97.8% 2340647 180.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,175,124 A→C L91L (CTT→CTG)  parE ← DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,175,1240AC100.0% 135.8 / NA 38L91L (CTT→CTG) parEDNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (38/0);  total (38/0)

GCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCAAAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTAT  >  NC_000913/3174892‑3175320
                                                                                                                                                                                                                                        |                                                                                                                                                                                                    
gCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCCCGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGCAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATc                                                                                                                                                                                                >  1:408827/1‑239 (MQ=255)
gCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATc                                                                                                                                                                                                >  1:393174/1‑239 (MQ=255)
     aaGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTc                                                                                                                                                                                           >  1:850447/1‑239 (MQ=255)
                 gTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTac                                                                                                                                                                               >  1:541412/1‑239 (MQ=255)
                  tACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTCATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTac                                                                                                                                                                               >  1:646848/1‑238 (MQ=255)
                       gTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGAACAGAATCAGTTCAACCGCCGGTacac                                                                                                                                                                             >  1:181829/1‑235 (MQ=255)
                         aTTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCctct                                                                                                                                                                        >  1:370886/1‑238 (MQ=255)
                           tGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTcc                                                                                                                                                                     >  1:59744/1‑239 (MQ=255)
                             cGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCgg                                                                                                                                                                   >  1:993515/1‑239 (MQ=255)
                                  aCCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGaa                                                                                                                                                              >  1:916906/1‑239 (MQ=255)
                                  aCCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGaa                                                                                                                                                              >  1:288554/1‑239 (MQ=255)
                                                               gCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCTCCGGTACACCCTCTTCCGGGTGAata                                                                                                                                                            >  1:906762/1‑212 (MQ=255)
                                                                 aCCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAAt                                                                                                                               >  1:725022/1‑239 (MQ=255)
                                                                     tttCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACt                                                                                                                           >  1:382505/1‑239 (MQ=255)
                                                                                                aaaCCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTaaaa                                                                                                  >  1:259184/1‑237 (MQ=255)
                                                                                                    cTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGACTATCCACCGGCATCCCGCGCCCATCGCCACTACCTTCTAACGACTGTTCAGCATGTAAAATAACg                                                                                             >  2:1057757/1‑238 (MQ=255)
                                                                                                      gACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACAccc                                                                                                                                                                           >  1:786534/1‑158 (MQ=255)
                                                                                                      gACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTaaaa                                                                                                  >  1:273026/1‑231 (MQ=255)
                                                                                                      gACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTACAATAACGTcc                                                                                          >  2:684324/1‑239 (MQ=255)
                                                                                                      gACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTcc                                                                                          >  2:1132007/1‑239 (MQ=255)
                                                                                                       aCCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTcc                                                                                          >  1:273610/1‑238 (MQ=255)
                                                                                                            cgcgGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGt                                                                                    >  1:661136/1‑239 (MQ=255)
                                                                                                                     cGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCCATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTg                                                                            >  1:595561/1‑238 (MQ=255)
                                                                                                                                aCGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGt                                                                  >  1:531305/1‑237 (MQ=255)
                                                                                                                                aCGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGt                                                                  >  2:630082/1‑237 (MQ=255)
                                                                                                                                      ttCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCAt                                                           >  1:1132720/1‑238 (MQ=255)
                                                                                                                                        cGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATcc                                                         >  2:483988/1‑238 (MQ=255)
                                                                                                                                                cGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTa                                                >  2:840966/1‑239 (MQ=255)
                                                                                                                                                     accaccGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAAt                                           >  1:882535/1‑239 (MQ=255)
                                                                                                                                                                    aCGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTaa                                                                                                    >  1:457398/1‑167 (MQ=255)
                                                                                                                                                                                ccgccAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACTTCCACGCGTTTTGCGCGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCg                >  1:560320/1‑239 (MQ=255)
                                                                                                                                                                                    cAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAggg                  >  1:633126/1‑233 (MQ=255)
                                                                                                                                                                                    cAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTg            >  1:923239/1‑239 (MQ=255)
                                                                                                                                                                                             ggTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGt    >  1:149995/1‑238 (MQ=255)
                                                                                                                                                                                              gTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCa                                             >  1:789332/1‑196 (MQ=255)
                                                                                                                                                                                              gTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGtat  >  1:1002544/1‑239 (MQ=255)
                                                                                                                                                                                                                           catgcaGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGa                                         >  1:649236/1‑171 (MQ=255)
                                                                                                                                                                                                                                  aCGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCcaca                                                      >  1:48849/1‑151 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                    
GCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCAAAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTAT  >  NC_000913/3174892‑3175320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A