Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F70 I1 R1	16	113.5	2638332	93.5%	2466840	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,018,810	(T)_7→6	coding (109/810 nt)	mgt ←	Monofunctional glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,018,804	0	T	.	97.0%	443.5 / 4.7	99	coding (115/810 nt)	mgt	Monofunctional glycosyltransferase
Reads supporting (aligned to +/- strand): ref base T (3/0); new base . (39/57); total (42/57)
Fisher's exact test for biased strand distribution p-value = 7.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 108 total aligned reads displayed.

AGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCTCCTTCTTTTAAAA  >  USA300TCH1516_ALE/2018664‑2018940

aGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACt                                                                                                                                          <  2:132603/141‑1 (MQ=255)

   tGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACtttt                                                                                                                                       <  1:780250/141‑1 (MQ=255)

    gACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAAGATTTTTAATAGTATTCGTTTACttttt                                                                                                                                      <  3:348566/141‑1 (MQ=255)

     acacAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACtttttt                                                                                                                                     >  3:248175/1‑141 (MQ=255)

     acacAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACtttttt                                                                                                                                     <  2:842827/141‑1 (MQ=255)

       acaAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACttttttct                                                                                                                                   >  1:155519/1‑139 (MQ=255)

       acaAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACttttttct                                                                                                                                   <  4:281100/141‑3 (MQ=255)

       acaAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACttttttct                                                                                                                                   <  1:177651/141‑3 (MQ=255)

              tactTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTcgg                                                                                                                           <  1:888627/141‑1 (MQ=255)

              tactTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTcgg                                                                                                                           >  1:508007/1‑141 (MQ=255)

                 ttttATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTtcgtcgt                                                                                                                        >  2:129194/1‑140 (MQ=255)

                  tttATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGt                                                                                                                       >  1:365639/1‑141 (MQ=255)

                     aTTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTg                                                                                                                    <  4:339312/141‑1 (MQ=255)

                     aTTTTCAATTTTTCTTAGTTCATCCACATTATCGCAAGAAAAAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTg                                                                                                                    <  4:39752/141‑1 (MQ=255)

                      ttttCAATTTTTCTTAGTTCATCCACATTATCTCGTGAAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGc                                                                                                                   <  4:196595/141‑1 (MQ=255)

                       tttCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGcc                                                                                                                  >  1:817565/1‑141 (MQ=255)

                       tttCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGcc                                                                                                                  <  1:721623/141‑1 (MQ=255)

                        ttCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCa                                                                                                                 <  3:149150/141‑1 (MQ=255)

                         tCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCaa                                                                                                                <  2:261615/141‑1 (MQ=255)

                         tCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCaa                                                                                                                <  1:469841/141‑1 (MQ=255)

                          cAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAAc                                                                                                               >  4:308854/1‑141 (MQ=255)

                          cAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAAc                                                                                                               >  1:855274/1‑141 (MQ=255)

                          cAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAACGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAAc                                                                                                               >  1:292712/1‑141 (MQ=255)

                           aaTTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACt                                                                                                              <  3:93650/141‑1 (MQ=255)

                            aTTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTg                                                                                                             <  1:88675/141‑1 (MQ=255)

                             tttttCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTgg                                                                                                            <  2:316761/141‑1 (MQ=255)

                                ttCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTg                                                                                                         >  1:490566/1‑141 (MQ=255)

                                 tCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGt                                                                                                           >  1:385039/1‑138 (MQ=255)

                                     aGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGataat                                                                                                    <  3:253258/141‑1 (MQ=255)

                                        tCATCCACATAATCGCGAATAGATAAAAAATACATAATACCAATAAGCAATGCTATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACg                                                                                                 <  2:648449/141‑1 (MQ=255)

                                          aTCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGta                                                                                               <  1:259249/141‑1 (MQ=255)

                                           tCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtat                                                                                              >  1:616095/1‑141 (MQ=255)

                                            ccACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatt                                                                                             <  4:298019/141‑1 (MQ=255)

                                               caTTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATtata                                                                                          <  1:532025/141‑1 (MQ=255)

                                                aTTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAg                                                                                         >  4:338902/1‑141 (MQ=255)

                                                 ttATCGCGTGTATATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGt                                                                                        <  2:673476/141‑1 (MQ=255)

                                                 ttATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGt                                                                                        <  3:29002/141‑1 (MQ=255)

                                                     cgcgTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAgg                                                                                    <  4:203964/141‑1 (MQ=255)

                                                      gcgTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGt                                                                                   >  4:210144/1‑141 (MQ=255)

                                                      gcgTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGt                                                                                   <  1:554817/141‑1 (MQ=255)

                                                        gtgtAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTc                                                                                 <  1:497448/141‑1 (MQ=255)

                                                        gtgtAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTc                                                                                 <  1:812147/141‑1 (MQ=255)

                                                          gtAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCgt                                                                               <  3:214797/141‑1 (MQ=255)

                                                           tAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCgtg                                                                              <  2:866279/141‑1 (MQ=255)

                                                            aGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGTATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGTTTGATACTACGTATTATAGTGAGGTTCgtga                                                                             >  2:102357/1‑140 (MQ=255)

                                                            aGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGTCGGTTTTCCAACTGGTTGATAATACGTATTATAGTGAGGTTCgtgt                                                                             >  4:275557/1‑141 (MQ=255)

                                                            aGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCgtgt                                                                             <  1:893630/141‑1 (MQ=255)

                                                             gATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGtt                                                                            >  4:61264/1‑141 (MQ=255)

                                                                  aaaaTACATGATAACAATAAACAATTCGATAATGATTAGAATTGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCata                                                                       <  4:248175/141‑1 (MQ=255)

                                                                   aaaTACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATAGTTTTAATCGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCctat                                                                      >  1:536190/1‑141 (MQ=255)

                                                                     aTACATGATACCACTAAACTATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGc                                                                    <  1:741136/141‑1 (MQ=255)

                                                                     aTACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGTTAATACGTATTATAGTGAGGTTCGTGTTTCATATGc                                                                    <  2:276738/141‑1 (MQ=255)

                                                                        cATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCa                                                                 >  1:768946/1‑141 (MQ=255)

                                                                        cATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTAATATGCTCa                                                                 >  3:95008/1‑141 (MQ=255)

                                                                          tGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCaaa                                                               <  2:549000/141‑1 (MQ=255)

                                                                             tACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATg                                                            <  2:566860/141‑1 (MQ=255)

                                                                                  aTAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCAt                                                       >  2:300600/1‑141 (MQ=255)

                                                                                  aTAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCAt                                                       >  3:310611/1‑141 (MQ=255)

                                                                                    aaaCAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCAttt                                                     >  3:374932/1‑141 (MQ=255)

                                                                                    aaaCAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCAttt                                                     >  3:42168/1‑141 (MQ=255)

                                                                                       cAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCAtttgag                                                  <  1:520712/141‑1 (MQ=255)

                                                                                          tGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTTGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCAtttgagttt                                               >  4:204875/1‑141 (MQ=255)

                                                                                           gCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATtata                                                                                          >  2:483027/1‑97 (MQ=255)

                                                                                               tAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTa                                          >  3:257879/1‑141 (MQ=255)

                                                                                                aaTGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTAAAAATGTTCAATTGAGTTTGAGTAc                                         >  3:132105/1‑141 (MQ=255)

                                                                                                 aTGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCTGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTAcc                                        >  2:738887/1‑141 (MQ=255)

                                                                                                 aTGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTAcc                                        >  1:224678/1‑141 (MQ=255)

                                                                                                   gATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTa                                      <  3:210144/141‑1 (MQ=255)

                                                                                                    aTTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTAt                                     <  3:228421/141‑1 (MQ=255)

                                                                                                      tAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTc                                                                                 <  2:371215/95‑1 (MQ=255)

                                                                                                      tAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTc                                                                                 >  1:371215/1‑95 (MQ=255)

                                                                                                       aGAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGc                                  <  1:608818/141‑1 (MQ=255)

                                                                                                        gAATGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGct                                 <  1:641993/141‑1 (MQ=255)

                                                                                                          aTGGTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGcttc                               <  1:864061/141‑1 (MQ=255)

                                                                                                             gTTAATAATATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCttt                            >  3:79561/1‑141 (MQ=255)

                                                                                                               taataaTATTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTc                          <  1:126537/141‑1 (MQ=255)

                                                                                                                   aataTTTTTAATAGTATTCGTTTACTTTTTTT‑TTTTTTTTCTGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGc                      >  4:334453/1‑141 (MQ=255)

                                                                                                                   aataTTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTTTTTTTTTTTTCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGc                      >  4:41460/1‑141 (MQ=255)

                                                                                                                     tatTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGt                    <  1:387237/141‑1 (MQ=255)

                                                                                                                     tatTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGt                    <  2:422714/141‑1 (MQ=255)

                                                                                                                     tatTTTTAATAGTATTCGTTTAC‑GTTTTTCTTTTTCGGAGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTGATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGt                    <  2:422730/141‑1 (MQ=255)

                                                                                                                      atTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTcggcgg                                                                                                                        >  1:763720/1‑40 (MQ=255)

                                                                                                                      atTTTTAATAGTATTCGTTTAC‑TTTTTTCTTTTTcggcgg                                                                                                                        <  2:763720/40‑1 (MQ=255)

                                                                                                                        ttttAATTGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTACATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTg                 <  4:282003/141‑1 (MQ=255)

                                                                                                                        ttttAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGCGGTTCGAGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTg                 >  1:270342/1‑141 (MQ=255)

                                                                                                                         tttAATAGTATTCGTTTACTTTTTTT‑TTTTTTTTTTTTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTTAGTTTGTGTACTTATCTTTTCTTTTCATTCGttttt                >  2:611844/1‑139 (MQ=255)

                                                                                                                         tttAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGc                <  2:621747/141‑1 (MQ=255)

                                                                                                                         tttAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGc                >  1:801537/1‑141 (MQ=255)

                                                                                                                          ttAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCt               >  1:661535/1‑141 (MQ=255)

                                                                                                                          ttAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCt               <  2:176751/141‑1 (MQ=255)

                                                                                                                          ttAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGCTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCt               >  1:803173/1‑141 (MQ=255)

                                                                                                                           tAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCttt                            <  2:236892/127‑1 (MQ=255)

                                                                                                                           tAATAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCttt                            >  1:236892/1‑127 (MQ=255)

                                                                                                                            aaTAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCTcc             >  1:916566/1‑141 (MQ=255)

                                                                                                                              tAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCTCctt           <  2:485744/141‑1 (MQ=255)

                                                                                                                              tAGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCTCctt           <  4:310611/141‑1 (MQ=255)

                                                                                                                               aGTATTCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCTCcttc          <  3:360662/141‑1 (MQ=255)

                                                                                                                                   ttCGTTTAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCTCCTTCtttt      <  3:338902/141‑1 (MQ=255)

                                                                                                                                      gTTTACTTTTTTT‑TTTTTTTTCGGATTGCCAACTGGTTGATAATACGTATTATTGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCTTTTGAGTTTGAGTACATATTGCTTTTTTTTATTCGTTTGCTACTTTTTTTtaa   >  2:270143/1‑141 (MQ=255)

                                                                                                                                       tttAC‑TTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCTCCTTCTTTTaaaa  <  3:308854/141‑1 (MQ=255)

AGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCATATGCTCAAAATGTTCATTTGAGTTTGAGTACCTATCGCTTCTTTTCATGCGTTTGCTCCTTCTTTTAAAA  >  USA300TCH1516_ALE/2018664‑2018940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A