Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F106 I1 R1 27 57.4 1363720 95.8% 1306443 135.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA98.7% 234.4 / NA 76A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (37/38);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATT  >  USA300TCH1516_ALE/2279573‑2279835
                                                                                                                                    |                                                                                                                                  
ccGTTATTAGTTGATTCTACTTCTGGATTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACt                                                                                                                                  >  1:353248/1‑135 (MQ=255)
 cGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCTTTTGCATTATCAGTTGCTGATGTTACtg                                                                                                                                 <  1:443505/135‑1 (MQ=255)
      ttaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCCTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTCGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTgttgtt                                                                                                                            <  2:406528/135‑1 (MQ=255)
       tatttGATTCTACTTCTGGTTTACTAGTCACATCGTTATCCATTGTCGGACTGTTTGTTTAGGCCCCTACGGTAATCTTGTTCTTCGCTTGATGTTTTTCATTTGTATCATTGGTTGTGGATTTTACT‑TTGtttt                                                                                                                          <  2:102137/132‑2 (MQ=255)
          ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAc                                                                                                                        <  1:158632/135‑1 (MQ=255)
          ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAc                                                                                                                        <  2:92668/135‑1 (MQ=255)
          ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAc                                                                                                                        <  2:113391/135‑1 (MQ=255)
           tttttCTGCTTCTGGTTTACTAGTTACATTGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc                                                                                                                       <  2:142149/132‑1 (MQ=255)
           tgcttCTACTTCTCGTCTGCCTGGGACAGCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTATCTTGCGGTTTATTATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc                                                                                                                       <  1:145024/132‑1 (MQ=255)
           tGATTCTACTTCTGGTTTACTAGTTACATCGTTATGCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc                                                                                                                       >  2:610653/1‑135 (MQ=255)
           tGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc                                                                                                                       <  2:591128/135‑1 (MQ=255)
            gcttCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCt                                                                                                                      >  1:205749/3‑135 (MQ=255)
            gATTCTACTTCTGGTTTACTAGATACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCt                                                                                                                      >  1:503131/1‑135 (MQ=255)
              ttCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGt                                                                                                                    <  1:602430/135‑1 (MQ=255)
               tCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                   >  1:584371/1‑135 (MQ=255)
                 ttcTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGc                                                                                                                 <  2:29339/133‑1 (MQ=255)
                 tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGc                                                                                                                 <  2:465739/135‑1 (MQ=255)
                    ttCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                              <  1:514749/135‑1 (MQ=255)
                    ttCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                              <  2:668873/135‑1 (MQ=255)
                       tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                           >  1:526341/1‑135 (MQ=255)
                            tccTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat                                                                                                      >  1:209936/3‑135 (MQ=255)
                             aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtatt                                                                                                     <  1:581679/135‑1 (MQ=255)
                              cTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCGTCACtagta                                                                                                    <  1:382799/135‑1 (MQ=255)
                                aGTTGCATCGTTATCCATTGTCAGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGGATCACTattatt                                                                                                  >  1:36725/1‑135 (MQ=255)
                                 gTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAAGTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt                                                                                                 >  1:618931/1‑135 (MQ=255)
                                       tCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgtt                                                                                           <  2:547992/135‑1 (MQ=255)
                                           tgtCCATTGTCGGACTGTTTTTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTAGTGTTGTCg                                                                                       <  2:408102/133‑1 (MQ=255)
                                           tATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATTACTATTATTTGGTGTTGTCg                                                                                       <  1:50823/135‑1 (MQ=255)
                                             tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGga                                                                                     >  1:585706/1‑135 (MQ=255)
                                               ccttGGCGGTCTGTTTGTTGATGCATCTACACTAGAATTGTTATTATCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCTCGTAGACGTGGTTTTGTCGgaga                                                                                   <  2:255287/133‑1 (MQ=255)
                                               cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga                                                                                   <  2:671385/135‑1 (MQ=255)
                                               cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCAGTTGCCGCATCACTATTATTTGGAGTTGTCGgaga                                                                                   >  2:351332/1‑135 (MQ=255)
                                               cATTGTCGGACTGTTTGTTAATGCATCTACACTAGAATTGTTATAAGCATGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga                                                                                   >  1:7170/1‑135 (MQ=255)
                                                aTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGaa                                                                                  >  1:341821/1‑135 (MQ=255)
                                                 ttGTCGTACTGTTTGTTGATGCATCTAAACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGAATCACTATTATTTGGTGTTGTCGGAGAAg                                                                                 >  1:496497/1‑135 (MQ=255)
                                                    tCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt                                                                              >  1:544782/1‑135 (MQ=255)
                                                    tCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt                                                                              >  1:337798/1‑135 (MQ=255)
                                                     cGGACTGTTTGTTGATGCATATACACTAGAATTGTTATTAGCTTGCGGAATATCATTTGCATCACCAGAGGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTc                                                                             >  1:456626/1‑135 (MQ=255)
                                                      ggACTGTTTGTTGATGCATCTTCACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCt                                                                            <  2:480178/135‑1 (MQ=255)
                                                       gccTGTTTGTTGATTCATCTACACTAGCATTGTTATTGGCATGCGATTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAGGCGTCtc                                                                           >  1:99998/3‑134 (MQ=255)
                                                        aCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGc                                                                          >  1:594442/1‑135 (MQ=255)
                                                        aCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTACGGTTTATCAATTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGGGAGGCGTCtac                                                                          >  1:79374/1‑133 (MQ=255)
                                                              tgttgCTGCATCTACACTAAAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGcc                                                                    >  1:516874/1‑135 (MQ=255)
                                                                 tgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAtt                                                                 >  2:325035/1‑135 (MQ=255)
                                                                  gATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTa                                                                >  2:530356/1‑135 (MQ=255)
                                                                  gATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTa                                                                <  1:401695/135‑1 (MQ=255)
                                                                  gATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTa                                                                >  2:326469/1‑135 (MQ=255)
                                                                   aTGCTTCTACACTAGAATTGTTATTAGCTTGCGGATTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg                                                               >  1:227815/1‑135 (MQ=255)
                                                                   aTGCATCTACACTAGAATTGTTATTAACTTACGGTTTATCATTTGCATAATCAGTAGCTGATGCTACTGTTGCTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg                                                               >  1:616309/1‑135 (MQ=255)
                                                                     gCATCTACACTAGAAATGTTATTAACTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCt                                                             >  1:332724/1‑135 (MQ=255)
                                                                             acTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtctc                                                     >  1:43599/1‑135 (MQ=255)
                                                                             acTAGAATTGTTATTAGCTTGCGGTTTAACATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtctc                                                     >  1:15442/1‑135 (MQ=255)
                                                                                   aTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGTGTTTGCTTTCATAACAGCTGTCGTCTCAGATAc                                               <  1:273012/135‑1 (MQ=255)
                                                                                    tttttattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTTGGAGAAGCGTCTGCTTTGCTATTAGCTGTCGTCTCAGATACg                                              <  1:215606/132‑1 (MQ=255)
                                                                                     tGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt                                             <  1:286460/135‑1 (MQ=255)
                                                                                          ttaGCTTGCGGTTTATCATTTGCATCCTCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGt                                        <  2:156910/135‑1 (MQ=255)
                                                                                                  cGGTTTTTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCTCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGt                                <  2:48299/135‑1 (MQ=255)
                                                                                                  cGGCTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGt                                <  2:476997/135‑1 (MQ=255)
                                                                                                    gTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGGTAGGTTGTCCAGTAt                              >  2:121804/1‑135 (MQ=255)
                                                                                                    gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTTTCGTCTCAGATGCGTTAGGTTGTCAAGTAt                              >  2:122202/1‑135 (MQ=255)
                                                                                                    gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTTTCAGATACGTTAGGTTGTCCAGTAt                              <  1:640607/135‑1 (MQ=255)
                                                                                                     tttATCATTTGCATGATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAGTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtt                             >  1:380456/1‑135 (MQ=255)
                                                                                                     tttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtt                             >  2:203748/1‑135 (MQ=255)
                                                                                                           ctttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTgg                       <  2:521134/134‑1 (MQ=255)
                                                                                                           aTTTGCATCATCAGTTGCAGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTgg                       <  1:368359/135‑1 (MQ=255)
                                                                                                            tttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                      <  2:585706/135‑1 (MQ=255)
                                                                                                            tttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCAGCATCACTATTATTTGGAGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTGTTTTCTGgt                      >  1:170991/1‑135 (MQ=255)
                                                                                                            tttGCATCATCAGTTGCTAAAATAACAGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                      >  1:481065/1‑135 (MQ=255)
                                                                                                             ttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtg                     <  2:459602/135‑1 (MQ=255)
                                                                                                                catcatCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTg                  >  2:602551/1‑135 (MQ=255)
                                                                                                                catcatCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTg                  >  2:176004/1‑135 (MQ=255)
                                                                                                                        gTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCa          >  1:180129/1‑135 (MQ=255)
                                                                                                                          tGCTGATGCTACTGGTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAtt        <  2:209789/135‑1 (MQ=255)
                                                                                                                              taTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa    <  2:366820/134‑1 (MQ=255)
                                                                                                                              gATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTTTTATGTGGTGATGTTGGATAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa    <  2:634056/135‑1 (MQ=255)
                                                                                                                                tGTTACTGTTGTTTCACCTGTTGCCTCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt  <  2:227890/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
CCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATT  >  USA300TCH1516_ALE/2279573‑2279835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A