Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
711,161 |
C→T |
13.7% |
intergenic (‑93/+196) |
fur ← / ← fldA |
DNA‑binding transcriptional dual regulator/flavodoxin 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 711,161 | 0 | C | T | 13.7%
| 139.5
/ 11.7
| 51 | intergenic (‑93/+196) | fur/fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
| Reads supporting (aligned to +/- strand): ref base C (18/26); new base T (2/5); total (20/31) |
| Fisher's exact test for biased strand distribution p-value = 6.90e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAAC > W3110S.gb/711097‑711231
|
cACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTa < 1:351494/70‑1 (MQ=255)
cTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAAt > 1:824989/1‑71 (MQ=255)
cATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATg < 1:4208824/69‑1 (MQ=255)
cATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa > 1:22351/1‑70 (MQ=255)
aTTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaa < 1:4465391/71‑1 (MQ=255)
ttAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaaa > 1:3656884/1‑71 (MQ=255)
agaagaAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAc > 1:4514626/1‑70 (MQ=255)
gACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa < 1:4322025/57‑1 (MQ=255)
aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTccc < 1:429856/70‑1 (MQ=255)
aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaaa > 1:2855099/1‑53 (MQ=255)
cAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTccc < 1:1280913/69‑1 (MQ=255)
gAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTgg > 1:4266469/1‑70 (MQ=255)
aTTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCAc > 1:3439759/1‑41 (MQ=255)
tGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTc > 1:904217/1‑60 (MQ=255)
ctctTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAatat < 1:3779806/70‑1 (MQ=255)
tctTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc < 1:3029215/59‑1 (MQ=255)
ctTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCt < 1:3918144/59‑1 (MQ=255)
ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaa < 1:435707/45‑1 (MQ=255)
ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaa < 1:2554206/45‑1 (MQ=255)
ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACcac < 1:957054/50‑1 (MQ=255)
ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTg < 1:1876226/69‑1 (MQ=255)
ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGt > 1:644259/1‑70 (MQ=255)
ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGt > 1:880753/1‑70 (MQ=255)
gagaTAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACcac > 1:1382428/1‑48 (MQ=255)
gagaTAATGCGTATCATTATAGAATTGCCACGCCTAAAt > 1:217369/1‑39 (MQ=255)
agaTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaa > 1:3430533/1‑70 (MQ=255)
agaTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaa > 1:2647644/1‑70 (MQ=255)
aTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaataa < 1:602390/71‑1 (MQ=255)
aTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaataa < 1:3081043/71‑1 (MQ=255)
tAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAatat < 1:4205212/61‑1 (MQ=255)
tAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaa < 1:649565/71‑1 (MQ=255)
tAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaa < 1:2255389/71‑1 (MQ=255)
aaTGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACaa > 1:3078717/1‑45 (MQ=255)
aaTGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAata < 1:553777/59‑1 (MQ=255)
tGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAc < 1:3057775/71‑1 (MQ=255)
aTCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTgg > 1:3026248/1‑70 (MQ=255)
tCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca < 1:2072511/71‑1 (MQ=255)
tCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca < 1:2243379/71‑1 (MQ=255)
cATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt < 1:1938202/52‑1 (MQ=255)
tataGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTg > 1:549432/1‑64 (MQ=255)
ataGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCt > 1:1049716/1‑71 (MQ=255)
gAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTa < 1:105370/70‑1 (MQ=255)
gAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTAc < 1:3855468/71‑1 (MQ=255)
aaTTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt > 1:2433384/1‑44 (MQ=255)
aTTGCCACGCCTAAATGAAAACCACAAGCCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACa < 1:1979981/70‑1 (MQ=255)
ttGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAata > 1:1108196/1‑40 (MQ=255)
gCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACAttt < 1:1660609/70‑1 (MQ=255)
ccATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTAc < 1:3507686/71‑1 (MQ=255)
ccACGCCTAAATGAAAACCACTAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTAc < 1:3110184/71‑1 (MQ=255)
ccACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGc < 1:4254604/56‑1 (MQ=255)
aCGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta > 1:1680846/1‑41 (MQ=255)
aCGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACaa < 1:2071607/71‑1 (MQ=255)
cGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaataa > 1:3144645/1‑44 (MQ=255)
cGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAAc > 1:4055013/1‑71 (MQ=255)
|
CACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAAC > W3110S.gb/711097‑711231
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
