Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,364,882 |
G→A |
5.9% |
V142V (GTG→GTA) |
puuC → |
gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,364,882 | 0 | G | A | 5.9%
| 224.7
/ 3.0
| 68 | V142V (GTG→GTA) | puuC | gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (37/27); new base A (2/2); total (39/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01 |
TGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGCCA > W3110S.gb/1364813‑1364946
|
tgatATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTg > 1:3703456/1‑70 (MQ=255)
atatTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGc < 1:4391032/70‑1 (MQ=255)
tatTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGa > 1:4165981/1‑71 (MQ=255)
ttCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGac < 1:1869418/70‑1 (MQ=255)
tCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGacca > 1:69444/1‑71 (MQ=255)
tCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGacca > 1:3646034/1‑71 (MQ=255)
cggcgcggcgCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGaccac > 1:198570/1‑69 (MQ=255)
ggcgcggcgcGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGAccaccac > 1:3707965/1‑70 (MQ=255)
gcgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAg > 1:1070406/1‑70 (MQ=255)
gcgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGt < 1:339453/71‑1 (MQ=255)
gcgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGt < 1:2845488/71‑1 (MQ=255)
cgcgcgcCATTCGCTTGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt < 1:1457736/71‑1 (MQ=255)
cgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt > 1:1341225/1‑71 (MQ=255)
cgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt < 1:2581137/71‑1 (MQ=255)
cgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt > 1:2818056/1‑69 (MQ=255)
cgcgcCATTCGCTGGTACGCAGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGa < 1:38094/71‑1 (MQ=255)
cATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTg > 1:535933/1‑70 (MQ=255)
ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGac < 1:1086785/49‑1 (MQ=255)
ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGc > 1:4384397/1‑70 (MQ=255)
ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGc > 1:1854152/1‑70 (MQ=255)
ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCg < 1:937086/71‑1 (MQ=255)
ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCg < 1:1759536/71‑1 (MQ=255)
tCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCga > 1:373525/1‑71 (MQ=255)
cGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGAccacca > 1:431214/1‑52 (MQ=255)
gCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgat < 1:2054728/70‑1 (MQ=255)
gCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgat > 1:4586267/1‑70 (MQ=255)
gCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgat > 1:2023945/1‑70 (MQ=255)
tGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgatgat > 1:663505/1‑71 (MQ=255)
tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgt < 1:983435/71‑1 (MQ=255)
tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgt < 1:555095/71‑1 (MQ=255)
cGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgc > 1:4231604/1‑71 (MQ=255)
ccGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGc < 1:4059824/47‑1 (MQ=255)
ccGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt < 1:3980730/50‑1 (MQ=255)
ccGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgcgt > 1:2582483/1‑71 (MQ=255)
gAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgcgtg > 1:3590011/1‑70 (MQ=255)
aGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAAc > 1:4289279/1‑71 (MQ=255)
aGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAAc > 1:4284030/1‑71 (MQ=255)
aGCGATCGACAAAGTGTATGGCCAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAAc < 1:1345343/71‑1 (MQ=255)
gCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgatg < 1:118976/57‑1 (MQ=255)
gATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCt < 1:3255352/48‑1 (MQ=255)
aTCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtg < 1:1189815/60‑1 (MQ=255)
tCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGt > 1:3879616/1‑70 (MQ=255)
cGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTc > 1:3218741/1‑70 (MQ=255)
cGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCg > 1:1744948/1‑71 (MQ=255)
cGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCg > 1:3643089/1‑71 (MQ=255)
gACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAg > 1:1487074/1‑42 (MQ=255)
gACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGt < 1:4519072/68‑1 (MQ=255)
gACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCgg < 1:1225278/71‑1 (MQ=255)
aCAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtg > 1:890369/1‑56 (MQ=255)
aCAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGc < 1:1596907/71‑1 (MQ=255)
cAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGa < 1:1653350/60‑1 (MQ=255)
cAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCg > 1:3584054/1‑71 (MQ=255)
cAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCg > 1:2973669/1‑71 (MQ=255)
aaaGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCg > 1:3017239/1‑63 (MQ=255)
aaaGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCTGCGt < 1:817956/71‑1 (MQ=255)
aaaGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGt > 1:2333458/1‑71 (MQ=255)
gtgtATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGAt > 1:801925/1‑71 (MQ=255)
tgtATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGAtt < 1:3550479/71‑1 (MQ=255)
gtATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgatga < 1:87913/44‑1 (MQ=255)
aTGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgcc > 1:1898447/1‑71 (MQ=255)
tGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtg > 1:2606887/1‑46 (MQ=255)
tGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTTCGTGAACCGGTCGGCGTGATTgccg > 1:2355535/1‑71 (MQ=255)
gCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGAt > 1:3517486/1‑64 (MQ=255)
gCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgccgc > 1:685345/1‑70 (MQ=255)
gCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgccgc > 1:318675/1‑70 (MQ=255)
cGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGCCa > 1:2063246/1‑71 (MQ=255)
cGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGCCa < 1:4274172/71‑1 (MQ=255)
tGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgcc < 1:246374/62‑1 (MQ=255)
tGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTg < 1:1807882/60‑1 (MQ=255)
|
TGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGCCA > W3110S.gb/1364813‑1364946
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
