Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,413,523 |
G→A |
15.2% |
S47F (TCC→TTC) |
ydaO ← |
predicted C32 tRNA thiolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,413,523 | 0 | G | A | 15.2%
| 119.6
/ 12.0
| 46 | S47F (TCC→TTC) | ydaO | predicted C32 tRNA thiolase |
| Reads supporting (aligned to +/- strand): ref base G (24/15); new base A (3/4); total (27/19) |
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACG > W3110S.gb/1413455‑1413588
|
aaTTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGgag < 1:1590973/71‑1 (MQ=255)
gattgGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAgg > 1:558701/1‑70 (MQ=255)
ttgGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCa > 1:148710/1‑70 (MQ=255)
ggCGCGCTTTGCTGCAAATTGCGCAGCATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAAc > 1:3230226/1‑71 (MQ=255)
gcgcgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAAcc < 1:3544605/71‑1 (MQ=255)
gcgcgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAAc > 1:3065043/1‑70 (MQ=255)
cgcgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCa < 1:385461/71‑1 (MQ=255)
gcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCatg < 1:3436608/70‑1 (MQ=255)
cTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCatgat > 1:1891968/1‑71 (MQ=255)
ttGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGc > 1:2982630/1‑71 (MQ=255)
tgctgcAAATTGCGCAGAATCTCAAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACAATGATGc > 1:1413717/1‑70 (MQ=255)
gctgcAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCat > 1:1346353/1‑64 (MQ=255)
cAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCg > 1:3461837/1‑70 (MQ=255)
aaaTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGga > 1:2614015/1‑47 (MQ=255)
aaaTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAACCCATGATGCGATCGcc > 1:2224166/1‑71 (MQ=255)
aaTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCct < 1:4097311/71‑1 (MQ=255)
aTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCctt < 1:2820600/71‑1 (MQ=255)
ttGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGcc > 1:3100232/1‑68 (MQ=255)
ttGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCcttc < 1:2869855/71‑1 (MQ=255)
gcgcAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCcttct < 1:4517296/70‑1 (MQ=255)
gcgcAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCaaa > 1:3669681/1‑50 (MQ=255)
gAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGAGATCGCCTTCTTCAAt > 1:1650448/1‑70 (MQ=255)
aaTCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCttcttc < 1:88456/66‑1 (MQ=255)
aTCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCa > 1:2180245/1‑70 (MQ=255)
tcCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCata > 1:4611225/1‑69 (MQ=255)
tcCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATAtt > 1:2287359/1‑71 (MQ=255)
tcCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCg < 1:3471228/50‑1 (MQ=255)
cAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTg > 1:150542/1‑70 (MQ=255)
cATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGt < 1:1316951/71‑1 (MQ=255)
tGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGt > 1:1375779/1‑69 (MQ=255)
gTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGc < 1:3629170/71‑1 (MQ=255)
ataGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCaa > 1:348866/1‑71 (MQ=255)
taGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAAt < 1:4137564/71‑1 (MQ=255)
gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATg < 1:3601729/70‑1 (MQ=255)
cTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCata > 1:3402552/1‑54 (MQ=255)
cTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCaa < 1:4338655/67‑1 (MQ=255)
tCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCtt < 1:4206126/70‑1 (MQ=255)
tCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCt < 1:586502/69‑1 (MQ=255)
tCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTc < 1:762504/71‑1 (MQ=255)
tCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCt < 1:293102/69‑1 (MQ=255)
tttACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATg > 1:3915365/1‑64 (MQ=255)
tttACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCg > 1:2973483/1‑70 (MQ=255)
tttACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATATGATTGAAgaa > 1:1745376/1‑55 (MQ=37)
tACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGcc > 1:2820244/1‑70 (MQ=255)
tACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGcc > 1:1171672/1‑70 (MQ=255)
aCCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGccc < 1:326326/70‑1 (MQ=255)
aCCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCa > 1:1763566/1‑71 (MQ=255)
ccccGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACg > 1:4499022/1‑71 (MQ=255)
ggAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAg > 1:3770679/1‑50 (MQ=255)
|
AATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACG > W3110S.gb/1413455‑1413588
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
