Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,420,329 |
+C |
intergenic (‑386/‑56) |
kil ← / → sieB |
inhibitor of ftsZ, killing protein/phage superinfection exclusion protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,420,325 | 1 | . | C | 79.2%
| 62.2
/ 12.2
| 24 | intergenic (‑382/‑60) | kil/sieB | inhibitor of ftsZ, killing protein/phage superinfection exclusion protein |
| Reads supporting (aligned to +/- strand): ref base . (0/5); new base C (0/19); total (0/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTG > W3110S.gb/1420283‑1420372
|
ggTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:964335/62‑1 (MQ=255)
ggTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:864461/62‑1 (MQ=255)
ggTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:1342421/62‑1 (MQ=255)
ggTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:1671889/62‑1 (MQ=255)
caccTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:1661679/49‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2061772/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:835574/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:736690/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:645449/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:515139/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2820956/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2713658/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2674540/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2514259/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1047443/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:190992/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1755601/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1544578/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:15111/62‑1 (MQ=255)
tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:150610/62‑1 (MQ=255)
ggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1908064/61‑1 (MQ=255)
ggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:371740/61‑1 (MQ=255)
ggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1320862/61‑1 (MQ=255)
gggctcGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1309960/58‑1 (MQ=255)
|
GGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTG > W3110S.gb/1420283‑1420372
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
