Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,683,257 |
G→A |
17.1% |
D190N (GAT→AAT) |
folM → |
dihydrofolate reductase isozyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,683,257 | 0 | G | A | 17.1%
| 82.4
/ 10.3
| 35 | D190N (GAT→AAT) | folM | dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base G (13/16); new base A (2/4); total (15/20) |
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 |
GCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAG > W3110S.gb/1683188‑1683323
|
gCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatg < 1:1885887/70‑1 (MQ=255)
cccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgat < 1:857023/71‑1 (MQ=255)
cccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgat < 1:2608846/71‑1 (MQ=255)
ccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatg < 1:4580393/71‑1 (MQ=255)
cGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatg < 1:3006128/70‑1 (MQ=255)
gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgat < 1:4427183/71‑1 (MQ=255)
gCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgat > 1:331470/1‑71 (MQ=255)
cAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgatg > 1:2551219/1‑71 (MQ=255)
ggCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGaa > 1:4109880/1‑70 (MQ=255)
gCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAata < 1:186745/71‑1 (MQ=255)
gaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAgg > 1:1552299/1‑71 (MQ=255)
agagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGgc > 1:2865452/3‑71 (MQ=255)
aagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga > 1:1595426/1‑49 (MQ=255)
agtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga > 1:369516/1‑48 (MQ=255)
tgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCg > 1:715910/1‑51 (MQ=255)
gaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACataat > 1:2005845/1‑43 (MQ=255)
tCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATa > 1:1058434/1‑70 (MQ=255)
tCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATa > 1:1808092/1‑70 (MQ=255)
cTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATaaa > 1:1777249/1‑71 (MQ=255)
cTATTGCGCCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGa > 1:1709589/1‑53 (MQ=255)
tATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga < 1:399819/39‑1 (MQ=255)
tATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATaa < 1:3914995/69‑1 (MQ=255)
ttGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAat < 1:4138624/45‑1 (MQ=255)
ttGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATc > 1:2707383/1‑70 (MQ=255)
cgcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAata < 1:4125130/43‑1 (MQ=255)
cgcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACt < 1:3280493/70‑1 (MQ=255)
cgcCATCGCTGATCCTGTTTAATGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtg < 1:2433246/71‑1 (MQ=255)
ccATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgat < 1:444256/71‑1 (MQ=255)
aTCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatga < 1:1356547/71‑1 (MQ=255)
aTCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatga < 1:246031/71‑1 (MQ=255)
tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgatga > 1:734101/1‑70 (MQ=255)
tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:2038623/71‑1 (MQ=255)
tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa < 1:4316036/71‑1 (MQ=255)
cGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATaaa > 1:3077675/1‑59 (MQ=255)
gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaa < 1:200390/70‑1 (MQ=255)
aTCCTGTTTAATGAACATGATGATGCCGAATATCGa < 1:3835566/36‑1 (MQ=255)
ccTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAAt < 1:3949745/53‑1 (MQ=255)
ccTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCgcgc > 1:856424/1‑71 (MQ=255)
tttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATa < 1:1400436/46‑1 (MQ=255)
tttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTg < 1:1034170/70‑1 (MQ=255)
ttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCgcgc > 1:1616250/1‑66 (MQ=255)
aTGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgag < 1:27150/71‑1 (MQ=255)
tGAACATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgaga < 1:2875475/71‑1 (MQ=255)
aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCga > 1:2383279/1‑66 (MQ=255)
aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaag < 1:2094050/71‑1 (MQ=255)
aCATAATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaag < 1:3429331/71‑1 (MQ=255)
cATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGagaaag > 1:1603077/1‑70 (MQ=255)
tgatgatgCCGAATATCGACAACAGGCGCTGAATa < 1:3339483/35‑1 (MQ=255)
gatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCt < 1:2088166/57‑1 (MQ=255)
|
GCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAG > W3110S.gb/1683188‑1683323
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
