Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,719,037 |
G→A |
17.1% |
V143V (GTG→GTA) |
yfiQ → |
fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,719,037 | 0 | G | A | 17.1%
| 100.8
/ 13.0
| 41 | V143V (GTG→GTA) | yfiQ | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/19); new base A (3/4); total (18/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
CCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACT > W3110S.gb/2718968‑2719104
|
ccTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGc < 1:2613357/71‑1 (MQ=255)
cTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGcc < 1:2642010/71‑1 (MQ=255)
cTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGcc < 1:1015881/71‑1 (MQ=255)
tGCTTGGACCAAACAGTATGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGcc > 1:2238965/1‑70 (MQ=255)
ggACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:1045626/70‑1 (MQ=255)
ggACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:606862/70‑1 (MQ=255)
ccAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAcg > 1:355998/1‑71 (MQ=255)
aaaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAAcgcg < 1:4059241/71‑1 (MQ=255)
aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:3921294/64‑1 (MQ=255)
aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCgg > 1:4301439/1‑71 (MQ=255)
aCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCgg > 1:4346841/1‑70 (MQ=255)
aCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGAATAAAcgcg > 1:4306092/1‑69 (MQ=255)
tCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGc < 1:3897611/71‑1 (MQ=255)
tGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTg > 1:1594585/1‑71 (MQ=255)
tACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAcgc > 1:2007754/1‑56 (MQ=255)
tACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGtt > 1:1088705/1‑70 (MQ=255)
ggCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTAtt > 1:672736/1‑70 (MQ=255)
gCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTAtt < 1:4581609/69‑1 (MQ=255)
ccTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCt < 1:3342275/55‑1 (MQ=255)
cTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt < 1:206590/71‑1 (MQ=255)
tGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGAt < 1:903232/38‑1 (MQ=255)
gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATc < 1:2290292/69‑1 (MQ=255)
gCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCg > 1:3269524/1‑70 (MQ=255)
aaGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:2574635/36‑1 (MQ=255)
ggTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTAt < 1:1629961/56‑1 (MQ=255)
tCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAc < 1:2522459/35‑1 (MQ=255)
cTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTAtt > 1:3201846/1‑54 (MQ=255)
cTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGtt < 1:1292151/50‑1 (MQ=255)
aaTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtctc > 1:1145792/1‑71 (MQ=255)
aaTGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtctc > 1:3877211/1‑71 (MQ=255)
aTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTcc < 1:2596342/71‑1 (MQ=255)
tGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtctc > 1:3017399/1‑69 (MQ=255)
ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCaa < 1:1871908/70‑1 (MQ=255)
cAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtct < 1:2621484/65‑1 (MQ=255)
aGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtct > 1:2954945/1‑64 (MQ=255)
gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAacac > 1:2358301/1‑70 (MQ=255)
cTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCa < 1:4151577/71‑1 (MQ=255)
cTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCa < 1:1643067/71‑1 (MQ=255)
ttttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAt > 1:3489392/1‑71 (MQ=255)
tCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCt > 1:1809167/1‑71 (MQ=255)
cTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGAc < 1:2106886/71‑1 (MQ=255)
tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACt < 1:2451593/69‑1 (MQ=255)
|
CCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACT > W3110S.gb/2718968‑2719104
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
