Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,951,877 |
G→A |
6.8% |
A928V (GCT→GTT) |
recB ← |
exonuclease V (RecBCD complex), beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,951,877 | 0 | G | A | 6.8%
| 233.8
/ 5.0
| 73 | A928V (GCT→GTT) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (36/32); new base A (2/3); total (38/35) |
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 |
GGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTA > W3110S.gb/2951808‑2951944
|
ggTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAAAGACGCTGGCAACGCCTgcagc < 1:3226302/71‑1 (MQ=255)
gTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcagca < 1:1288309/71‑1 (MQ=255)
gTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcaaca < 1:3232997/71‑1 (MQ=255)
gACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc > 1:1513721/1‑71 (MQ=255)
aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACAt > 1:1673003/1‑71 (MQ=255)
gcgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATc > 1:2512268/1‑69 (MQ=255)
ggAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:2305506/70‑1 (MQ=255)
aaaCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAg > 1:3045005/1‑71 (MQ=255)
tGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACAt > 1:1837598/1‑58 (MQ=255)
aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATc > 1:1940694/1‑70 (MQ=255)
aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATc < 1:4128131/70‑1 (MQ=255)
tgtgGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCa > 1:1236620/1‑70 (MQ=255)
tgtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt > 1:1100537/1‑71 (MQ=255)
tgtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt > 1:3063817/1‑71 (MQ=255)
tgtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt < 1:921802/71‑1 (MQ=255)
tgtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCt < 1:597560/71‑1 (MQ=255)
gtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAg < 1:949905/58‑1 (MQ=255)
ttAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCa < 1:2104561/63‑1 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGa > 1:1998254/1‑54 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGc > 1:862568/1‑71 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCACCCGAGGCATCAAATCCTGGGc > 1:410971/1‑71 (MQ=255)
cGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGa > 1:4310832/1‑71 (MQ=255)
gTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGAt < 1:3744279/71‑1 (MQ=255)
cGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATAc > 1:3837762/1‑71 (MQ=255)
ggTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCaa > 1:2732000/1‑56 (MQ=255)
ggTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATAcc > 1:1485063/1‑71 (MQ=255)
ggTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTg < 1:3268711/62‑1 (MQ=255)
gTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCg < 1:3844001/71‑1 (MQ=255)
gTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATc > 1:3065334/1‑58 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATc < 1:2008777/57‑1 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:4419994/71‑1 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:933213/71‑1 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:4434778/71‑1 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:3484423/71‑1 (MQ=255)
tcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt > 1:2783076/1‑70 (MQ=255)
tcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt > 1:4468824/1‑70 (MQ=255)
cttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGa > 1:448918/1‑44 (MQ=255)
tcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAc > 1:1719323/1‑70 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTg < 1:3695344/55‑1 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAcc < 1:1336076/70‑1 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAc < 1:159247/69‑1 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATACAGCCGAGGCATCAAATCCTg < 1:1686209/55‑1 (MQ=255)
aaCGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt < 1:2845138/53‑1 (MQ=255)
acgacgCTGGCAACGCCTGCAGCATCGACATCAAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACg > 1:1390546/1‑71 (MQ=255)
cgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGa > 1:842361/1‑57 (MQ=255)
cgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGc > 1:942023/1‑71 (MQ=255)
cgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGc < 1:238887/71‑1 (MQ=255)
gacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgga > 1:3227710/1‑39 (MQ=255)
gacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCt < 1:2813839/71‑1 (MQ=255)
gacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCt < 1:996566/71‑1 (MQ=255)
acgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGc > 1:4468753/1‑39 (MQ=255)
acgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTg < 1:868808/71‑1 (MQ=255)
acgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTg < 1:1143069/71‑1 (MQ=255)
acgCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTg < 1:3541470/71‑1 (MQ=255)
cgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGt < 1:3578991/71‑1 (MQ=255)
cgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGt < 1:3195975/71‑1 (MQ=255)
gCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATcc > 1:3954324/1‑46 (MQ=255)
cTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTg < 1:1542637/71‑1 (MQ=255)
cTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTg > 1:1864046/1‑71 (MQ=255)
tGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCg > 1:1888447/1‑56 (MQ=255)
gCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCg < 1:4530258/54‑1 (MQ=255)
gCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCa > 1:3955084/1‑70 (MQ=255)
gCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCa > 1:4075723/1‑70 (MQ=255)
aaCGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTg > 1:1020327/1‑54 (MQ=255)
cGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCaaa > 1:220708/1‑68 (MQ=255)
cGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCa < 1:4314943/71‑1 (MQ=255)
cGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCa < 1:2621859/71‑1 (MQ=255)
gCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCag < 1:1941353/71‑1 (MQ=255)
ccTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGt > 1:1719946/1‑60 (MQ=255)
ccTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCaga < 1:4300510/71‑1 (MQ=255)
cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATAcc < 1:2821657/46‑1 (MQ=255)
cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCa > 1:634707/1‑63 (MQ=255)
cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCagag > 1:3638036/1‑71 (MQ=255)
cTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCaga > 1:1336670/1‑70 (MQ=255)
tGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGtt > 1:2102890/1‑59 (MQ=255)
tGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCa < 1:3730080/62‑1 (MQ=255)
gcagcaTCGACATCCAGCCAAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCaaa < 1:1521639/63‑1 (MQ=255)
gcaacaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGtt > 1:2908658/1‑58 (MQ=255)
cagcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTa < 1:688587/70‑1 (MQ=255)
|
GGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTA > W3110S.gb/2951808‑2951944
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
