Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,951,877 |
G→A |
28.3% |
A928V (GCT→GTT) |
recB ← |
exonuclease V (RecBCD complex), beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,951,877 | 0 | G | A | 28.3%
| 86.9
/ 37.3
| 53 | A928V (GCT→GTT) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (20/18); new base A (8/7); total (28/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 |
CGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGC > W3110S.gb/2951807‑2951946
|
cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:4699545/1‑71 (MQ=255)
cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:1459244/1‑71 (MQ=255)
cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:398116/1‑71 (MQ=255)
cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:2376513/1‑71 (MQ=255)
ggTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:5061671/1‑70 (MQ=255)
ggTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:3176567/1‑70 (MQ=255)
tGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCAt > 1:2655481/1‑71 (MQ=255)
aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc < 1:4602331/70‑1 (MQ=255)
aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc < 1:2609274/70‑1 (MQ=255)
aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCg < 1:4818902/71‑1 (MQ=255)
aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATc < 1:976131/70‑1 (MQ=255)
aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGa < 1:3252264/68‑1 (MQ=255)
aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGa < 1:2365209/68‑1 (MQ=255)
aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACAt > 1:1311832/1‑71 (MQ=255)
ccGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATc < 1:1222869/71‑1 (MQ=255)
gcgcgGAAACTGATGTTGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCa < 1:2538375/71‑1 (MQ=255)
gcgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc > 1:2826103/1‑63 (MQ=255)
gcgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc > 1:1690262/1‑63 (MQ=255)
gcgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCa < 1:1490761/71‑1 (MQ=255)
cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:238952/71‑1 (MQ=255)
cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGcc > 1:285843/1‑71 (MQ=255)
cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGcc > 1:4787436/1‑71 (MQ=255)
aaCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg < 1:155742/71‑1 (MQ=255)
aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCa < 1:3188753/71‑1 (MQ=255)
tgtgGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCaa < 1:1742645/71‑1 (MQ=255)
tggtgTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:1585024/59‑1 (MQ=255)
ttAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCt < 1:1937736/69‑1 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg > 1:3369229/1‑70 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg > 1:191040/1‑70 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg > 1:2203508/1‑70 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGc > 1:1223435/1‑71 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGc > 1:1990578/1‑71 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGc > 1:3033210/1‑71 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGc > 1:2712459/1‑71 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTggg < 1:4848315/70‑1 (MQ=255)
gTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGAt < 1:2662578/71‑1 (MQ=255)
tCGGTTCTTCAACGACGCTTGCAACGCCTGCAACATCGACATCCAGCCGAGGCa > 1:4497307/1‑54 (MQ=255)
tCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATa > 1:2347082/1‑71 (MQ=255)
tCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATa > 1:3005629/1‑71 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:1356566/71‑1 (MQ=255)
cttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGa > 1:1103755/1‑71 (MQ=255)
cttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCt < 1:3586626/57‑1 (MQ=255)
ttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCt < 1:942785/56‑1 (MQ=255)
tcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATcc > 1:308882/1‑54 (MQ=255)
tcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATcc > 1:3236722/1‑54 (MQ=255)
tcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAc > 1:4977533/1‑70 (MQ=255)
acgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACg > 1:2917222/1‑71 (MQ=255)
acgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACg > 1:3955861/1‑71 (MQ=255)
gacgCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCt > 1:3150395/1‑71 (MQ=255)
gCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGtt > 1:3584309/1‑71 (MQ=255)
gCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGtt > 1:63598/1‑71 (MQ=255)
aCGCCTGCAACATCGACATCCAGCCGAGGCATCAAATc > 1:3302693/1‑38 (MQ=255)
aCGCCTGCAACATCGACATCCAGCCGAGGCATCAAATc > 1:270840/1‑38 (MQ=255)
gCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCag < 1:1506439/71‑1 (MQ=255)
gCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCag < 1:1916894/71‑1 (MQ=255)
gCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCag < 1:4771044/71‑1 (MQ=255)
cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCagag < 1:4642779/71‑1 (MQ=255)
cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCagag > 1:2536446/1‑71 (MQ=255)
cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCagag < 1:2176746/71‑1 (MQ=255)
cagcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAg > 1:4227451/1‑71 (MQ=255)
agcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGc < 1:3517067/71‑1 (MQ=255)
agcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGc < 1:1322877/71‑1 (MQ=255)
|
CGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGC > W3110S.gb/2951807‑2951946
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
