Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,065,341 |
G→T |
10.1% |
I163I (ATC→ATA) |
ygfI ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,065,341 | 0 | G | T | 10.1%
| 220.5
/ 6.8
| 69 | I163I (ATC→ATA) | ygfI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (26/36); new base T (3/4); total (29/40) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 |
GCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTAA > W3110S.gb/3065271‑3065406
|
gctcttgctcttGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTg < 1:1377457/71‑1 (MQ=255)
gctcttgctcttGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTGCACTg < 1:1352528/71‑1 (MQ=255)
ctcttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa < 1:2279980/71‑1 (MQ=255)
tcttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:3529125/1‑70 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:809566/1‑69 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:1913061/1‑69 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:3932575/70‑1 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt > 1:3184130/1‑70 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:332175/70‑1 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt > 1:1203765/1‑70 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:1014182/70‑1 (MQ=255)
ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTc < 1:3876210/70‑1 (MQ=255)
ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTc < 1:1681244/70‑1 (MQ=255)
ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTc < 1:3572862/70‑1 (MQ=255)
ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTc < 1:424258/70‑1 (MQ=255)
tcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAg > 1:4611175/1‑71 (MQ=255)
ttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:3838615/1‑71 (MQ=255)
ttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:1986024/1‑71 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg < 1:1427651/68‑1 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg > 1:1104134/1‑68 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGttt > 1:924124/1‑71 (MQ=255)
ctctTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc > 1:1675762/1‑69 (MQ=255)
ctTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGt > 1:2904928/1‑71 (MQ=255)
ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:3943521/57‑1 (MQ=255)
tCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc > 1:22082/1‑71 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:294553/1‑60 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTcc > 1:1694210/1‑71 (MQ=255)
aTAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa < 1:3917386/50‑1 (MQ=255)
tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg < 1:4295985/71‑1 (MQ=255)
tAACGGCGAGAAACGAGAAAGACTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTg < 1:2720846/71‑1 (MQ=255)
aaCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc < 1:3146317/62‑1 (MQ=255)
aaCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg > 1:1563466/1‑70 (MQ=255)
cGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAAc < 1:3601514/71‑1 (MQ=255)
ggCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:2383971/49‑1 (MQ=255)
gCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTc > 1:3929438/1‑71 (MQ=255)
gagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGt < 1:1215688/71‑1 (MQ=255)
gagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGt < 1:3301759/71‑1 (MQ=255)
agaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg < 1:4144912/71‑1 (MQ=255)
agaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg > 1:2424210/1‑71 (MQ=255)
gaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc > 1:3062958/1‑53 (MQ=255)
gaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg < 1:1993708/70‑1 (MQ=255)
gaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg > 1:476674/1‑70 (MQ=255)
acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:1238111/1‑37 (MQ=255)
acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc < 1:2693570/56‑1 (MQ=255)
cgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGa > 1:2775108/1‑70 (MQ=255)
cgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:4109007/1‑45 (MQ=255)
aaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACAt > 1:4525425/1‑71 (MQ=255)
aGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGc < 1:705165/71‑1 (MQ=255)
gCCTAATCGCAGCGGTTGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCt < 1:4586514/71‑1 (MQ=255)
gCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCt < 1:2748749/71‑1 (MQ=255)
ccTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc < 1:2705631/41‑1 (MQ=255)
cTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAg < 1:3256789/60‑1 (MQ=255)
tAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtgtg > 1:2873045/1‑71 (MQ=255)
aaTCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCGGATACATGCTGTgg > 1:3211094/1‑71 (MQ=255)
tCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc < 1:2778509/36‑1 (MQ=255)
tCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCg < 1:3504381/51‑1 (MQ=255)
tggtAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGataa < 1:636245/68‑1 (MQ=255)
tggtAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtgt < 1:3117979/58‑1 (MQ=255)
tAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGACCTCGTGCAGATACATGCTGTggg < 1:1629056/58‑1 (MQ=255)
tAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtg < 1:1820927/54‑1 (MQ=255)
aGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATAc > 1:1003155/1‑47 (MQ=255)
gTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTc < 1:4493143/36‑1 (MQ=255)
gTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATaaa > 1:2074279/1‑67 (MQ=255)
gTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCt < 1:3972314/71‑1 (MQ=255)
ttCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:2639324/40‑1 (MQ=255)
ttCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATa < 1:3022154/44‑1 (MQ=255)
ttCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATc > 1:1942308/1‑69 (MQ=255)
tCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATaaaa < 1:4480405/66‑1 (MQ=255)
cACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTaa < 1:1169324/70‑1 (MQ=255)
cTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATg < 1:3352663/44‑1 (MQ=255)
cTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtgt < 1:4372834/48‑1 (MQ=255)
|
GCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTAA > W3110S.gb/3065271‑3065406
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
