Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,224,816 |
C→T |
8.3% |
H147Y (CAT→TAT) |
ebgC → |
cryptic beta‑D‑galactosidase, beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,224,816 | 0 | C | T | 8.3%
| 228.0
/ 8.2
| 72 | H147Y (CAT→TAT) | ebgC | cryptic beta‑D‑galactosidase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (41/25); new base T (4/2); total (45/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 |
TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAT > W3110S.gb/3224749‑3224884
|
tCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCtata < 1:271747/71‑1 (MQ=255)
ggTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataa < 1:1685032/70‑1 (MQ=255)
gTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataa > 1:3870687/1‑69 (MQ=255)
ttATCTGCAATACCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaa > 1:2260974/1‑70 (MQ=255)
ttATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaa > 1:3386773/1‑71 (MQ=255)
aTCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaat < 1:151694/70‑1 (MQ=255)
aTCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaat < 1:2635963/70‑1 (MQ=255)
cAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataacaa < 1:2766030/70‑1 (MQ=255)
cAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac < 1:442754/68‑1 (MQ=255)
cAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac < 1:3584420/68‑1 (MQ=255)
cAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATaacaac < 1:4003499/71‑1 (MQ=255)
cAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATaacaac < 1:3537210/71‑1 (MQ=255)
aaCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTAcg > 1:1453213/1‑71 (MQ=255)
aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTAcgg < 1:2751455/71‑1 (MQ=255)
cgcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg < 1:1032309/70‑1 (MQ=255)
cgcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaa > 1:4171840/1‑57 (MQ=255)
gcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaat < 1:3897496/58‑1 (MQ=255)
ggTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcggcgg > 1:3375135/1‑70 (MQ=255)
gTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACggcggc > 1:1263345/1‑70 (MQ=255)
gTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACt < 1:2124052/62‑1 (MQ=255)
tCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCTGCaa < 1:929530/71‑1 (MQ=255)
tCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaa < 1:194616/71‑1 (MQ=255)
aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCaa > 1:4040729/1‑69 (MQ=255)
aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataa > 1:3013143/1‑47 (MQ=255)
aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataa > 1:2553802/1‑47 (MQ=255)
aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaa > 1:834381/1‑69 (MQ=255)
aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaa > 1:990151/1‑69 (MQ=255)
aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaa > 1:1805135/1‑69 (MQ=255)
aaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac < 1:1377931/54‑1 (MQ=255)
aaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAg > 1:3684261/1‑71 (MQ=255)
aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAg > 1:1806231/1‑70 (MQ=255)
aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAg < 1:3387484/70‑1 (MQ=255)
aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAg > 1:1178370/1‑70 (MQ=255)
aaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataa > 1:3213510/1‑44 (MQ=255)
aaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAg > 1:655997/1‑69 (MQ=255)
aaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAg > 1:3097263/1‑69 (MQ=255)
aaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGa < 1:4413616/70‑1 (MQ=255)
aaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAg < 1:1091279/71‑1 (MQ=255)
aGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGt > 1:4158484/1‑71 (MQ=255)
gTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTAc < 1:3887623/55‑1 (MQ=255)
ttCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATGACAACTACGGCGGCa < 1:3002063/58‑1 (MQ=255)
ttCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaa < 1:3739539/41‑1 (MQ=255)
ttCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgc > 1:208016/1‑70 (MQ=255)
tctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACt < 1:2691943/48‑1 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTAcggcg > 1:2817067/1‑53 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaaaa > 1:2112750/1‑59 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAg > 1:728221/1‑63 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGttt > 1:1612026/1‑66 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgc > 1:285983/1‑71 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgc > 1:2104156/1‑71 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgc > 1:4085127/1‑71 (MQ=255)
tcAAAGTCACCATCGAAGATGGTTATTTCCataacaaat < 1:95163/39‑1 (MQ=255)
cAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATaacaac > 1:3071423/1‑44 (MQ=255)
aaaGTCACCATCGAAGATGGTTATTTCCataacaaataacaa > 1:151192/1‑42 (MQ=255)
aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGtt < 1:805882/62‑1 (MQ=255)
aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgcc < 1:3896650/69‑1 (MQ=255)
aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCAc > 1:4439396/1‑71 (MQ=255)
aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGCCAAAAGGAg > 1:4337260/1‑60 (MQ=255)
aaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCAcc < 1:2999868/71‑1 (MQ=255)
aGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACggcggc > 1:432734/1‑50 (MQ=255)
aGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACggcggc > 1:2478722/1‑50 (MQ=255)
gTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTAc < 1:2441164/43‑1 (MQ=255)
tCGAAGATGGTTATTTCCATAACAAATAACAACTa > 1:2244109/1‑35 (MQ=255)
cGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTAc > 1:2834917/1‑71 (MQ=255)
cGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTAc > 1:1660520/1‑71 (MQ=255)
gATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGa > 1:3908379/1‑44 (MQ=255)
gATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAt > 1:2838677/1‑71 (MQ=255)
ggTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAtttt > 1:4146026/1‑71 (MQ=255)
ttATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgcc > 1:1415234/1‑46 (MQ=255)
ttCCATAACAAATAACAACTACGGCGGCAAAAGGa > 1:3785432/1‑35 (MQ=255)
ttCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg > 1:4491756/1‑67 (MQ=255)
tCCATAACAAATAACAACTACGGCGGCAAAAGGAGttt > 1:3026130/1‑38 (MQ=255)
tCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAt > 1:1968460/1‑71 (MQ=255)
ccATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgcc > 1:976036/1‑43 (MQ=255)
|
TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGAT > W3110S.gb/3224749‑3224884
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
