Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,224,816 |
C→T |
31.2% |
H147Y (CAT→TAT) |
ebgC → |
cryptic beta‑D‑galactosidase, beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,224,816 | 0 | C | T | 31.2%
| 75.2
/ 37.8
| 52 | H147Y (CAT→TAT) | ebgC | cryptic beta‑D‑galactosidase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/11); new base T (8/8); total (33/19) |
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGG > W3110S.gb/3224749‑3224880
|
tCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTat < 1:503805/70‑1 (MQ=255)
ttATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaa > 1:3864767/1‑71 (MQ=255)
ttATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaa > 1:4370253/1‑71 (MQ=255)
ttATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaa > 1:356019/1‑71 (MQ=255)
cAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATaacaac < 1:1291798/71‑1 (MQ=255)
aTAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTa > 1:2630586/1‑71 (MQ=255)
aaCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTAcg > 1:4689746/1‑71 (MQ=255)
aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTataacaaataa > 1:4609022/1‑62 (MQ=255)
aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg > 1:3200796/1‑71 (MQ=255)
aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg > 1:46428/1‑71 (MQ=255)
gTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCa < 1:1891800/71‑1 (MQ=255)
aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataa > 1:4894565/1‑54 (MQ=255)
aaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAg > 1:4214105/1‑71 (MQ=255)
aaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTa > 1:774016/1‑56 (MQ=255)
gTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGtt < 1:5130091/71‑1 (MQ=255)
ggTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGttt < 1:2623580/70‑1 (MQ=255)
tctcAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAg < 1:2270638/64‑1 (MQ=255)
tctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataacaa > 1:1642020/1‑46 (MQ=255)
tctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac > 1:2635458/1‑44 (MQ=255)
tctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac > 1:3861205/1‑44 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCTataacaaataac < 1:3032025/43‑1 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCTataacaaataac < 1:1538712/43‑1 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac < 1:4076800/43‑1 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac < 1:1750649/43‑1 (MQ=255)
cAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCa > 1:4661731/1‑71 (MQ=255)
cAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCa > 1:1888306/1‑71 (MQ=255)
aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgcc < 1:3014014/69‑1 (MQ=255)
tCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGc > 1:549385/1‑70 (MQ=255)
aTCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTAccc > 1:1271553/1‑70 (MQ=255)
tCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaa < 1:1899189/44‑1 (MQ=255)
tCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTa > 1:1174104/1‑71 (MQ=255)
gAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTg > 1:941365/1‑52 (MQ=255)
gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgcc > 1:4495287/1‑54 (MQ=255)
gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgc > 1:2958825/1‑53 (MQ=255)
gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTa > 1:3808948/1‑64 (MQ=255)
gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTAc > 1:706306/1‑70 (MQ=255)
gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACt > 1:3178330/1‑71 (MQ=255)
gAAGATGGTTATTTCAATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgc > 1:3789666/1‑56 (MQ=255)
tGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGttt < 1:1205483/46‑1 (MQ=255)
gTTATGTCCATAACAAATAACAACTACGGCGGCaaaa > 1:2290676/1‑37 (MQ=255)
ttATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTg < 1:899925/44‑1 (MQ=255)
ttATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg < 1:3247604/71‑1 (MQ=255)
tATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCAc < 1:1115049/50‑1 (MQ=255)
aTTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgcc > 1:3223459/1‑44 (MQ=255)
aTTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGc > 1:2276814/1‑52 (MQ=255)
aTTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTc > 1:4893398/1‑68 (MQ=255)
aTTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg > 1:596489/1‑69 (MQ=255)
aTTTCCATAACAAATAACAACTACGGCGGCAAAAGGAg < 1:4299916/38‑1 (MQ=255)
tttCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCgg < 1:3121777/69‑1 (MQ=255)
tttCCATAACAAATAACAACTACGGCGGCAAAAGGa < 1:4572152/36‑1 (MQ=255)
ttCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg > 1:391645/1‑67 (MQ=255)
ttCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg > 1:3962795/1‑67 (MQ=255)
ccATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTAcc > 1:4479290/1‑52 (MQ=255)
|
TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGG > W3110S.gb/3224749‑3224880
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
