Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,332,615 |
T→A |
11.3% |
intergenic (‑25/+102) |
yhbE ← / ← rpmA |
conserved inner membrane protein/50S ribosomal subunit protein L27 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,332,615 | 0 | T | A | 11.3%
| 189.0
/ 15.8
| 71 | intergenic (‑25/+102) | yhbE/rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Reads supporting (aligned to +/- strand): ref base T (28/35); new base A (4/4); total (32/39) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01 |
CTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCG > W3110S.gb/3332545‑3332684
|
cTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCAttt < 1:3015660/71‑1 (MQ=255)
tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTg < 1:1820230/69‑1 (MQ=255)
tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGc < 1:1205504/70‑1 (MQ=255)
gtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGa > 1:1501544/1‑71 (MQ=255)
gAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt > 1:1304204/1‑70 (MQ=255)
aGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:489202/70‑1 (MQ=255)
gcgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTaa < 1:4057308/71‑1 (MQ=255)
cgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAAc < 1:3861070/71‑1 (MQ=255)
cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc < 1:538794/71‑1 (MQ=255)
cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc > 1:2765363/1‑71 (MQ=255)
aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCg > 1:4433435/1‑71 (MQ=255)
aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCg > 1:1768590/1‑71 (MQ=255)
aaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCg > 1:4079586/1‑70 (MQ=255)
aaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCa > 1:1452629/1‑71 (MQ=255)
gAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAcc < 1:1049844/71‑1 (MQ=255)
gAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAc > 1:3166635/1‑70 (MQ=255)
ccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAt > 1:1328817/1‑68 (MQ=255)
ccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcg < 1:4108968/71‑1 (MQ=255)
caatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcg > 1:4195488/1‑70 (MQ=255)
atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTaa > 1:4196276/1‑54 (MQ=255)
atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCa > 1:2436133/1‑64 (MQ=255)
atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:2761564/71‑1 (MQ=255)
atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:3495770/71‑1 (MQ=255)
atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt > 1:282979/1‑71 (MQ=255)
atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCAt < 1:2835740/71‑1 (MQ=255)
tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtt < 1:2680553/71‑1 (MQ=255)
tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtt < 1:3056681/71‑1 (MQ=255)
tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtt < 1:3075669/71‑1 (MQ=255)
cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt < 1:3472586/71‑1 (MQ=255)
cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt > 1:164298/1‑71 (MQ=255)
cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt < 1:544296/71‑1 (MQ=255)
ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAc < 1:2762809/58‑1 (MQ=255)
ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcgc < 1:1243248/65‑1 (MQ=255)
ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcgc < 1:4387989/65‑1 (MQ=255)
ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt > 1:4035572/1‑70 (MQ=255)
ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:2712001/67‑1 (MQ=255)
ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCAttttt < 1:2105963/71‑1 (MQ=255)
tgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTg > 1:1419660/1‑41 (MQ=255)
cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc < 1:2134198/70‑1 (MQ=255)
ctgctgCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt > 1:535564/1‑66 (MQ=255)
tgctgcTTCATACCGTATTCTCTGCAAAGCGCATTTGCGGAt < 1:3883794/42‑1 (MQ=255)
tgctgcTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt < 1:2501047/42‑1 (MQ=255)
gctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:1959243/61‑1 (MQ=255)
gctgctTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGt > 1:2472270/1‑71 (MQ=255)
ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCgg < 1:1492044/38‑1 (MQ=255)
ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGtt < 1:4240906/71‑1 (MQ=255)
ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGtt < 1:3520368/71‑1 (MQ=255)
ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGtt < 1:3515199/71‑1 (MQ=255)
gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTAt > 1:2016403/1‑71 (MQ=255)
ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:3551537/57‑1 (MQ=255)
ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTa > 1:2183502/1‑62 (MQ=255)
tCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAt > 1:1090082/1‑49 (MQ=255)
tCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTAt > 1:1279057/1‑68 (MQ=255)
cATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:154926/1‑71 (MQ=255)
tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt < 1:1380479/56‑1 (MQ=255)
aCCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCa > 1:1221017/1‑44 (MQ=255)
ccGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGt > 1:2738651/1‑70 (MQ=255)
ccGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTa < 1:4175929/71‑1 (MQ=255)
cGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaa < 1:1995016/71‑1 (MQ=255)
cGTATTCACTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTa > 1:1702118/1‑70 (MQ=255)
tATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa < 1:3192366/71‑1 (MQ=255)
ttCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAcc > 1:2932634/1‑52 (MQ=255)
ttCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAg < 1:46863/71‑1 (MQ=255)
cccTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGcc > 1:1663341/1‑71 (MQ=255)
cTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa > 1:2340012/1‑65 (MQ=255)
gCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCg > 1:2019688/1‑70 (MQ=255)
aaGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAAc < 1:1726543/71‑1 (MQ=255)
aGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAAca < 1:551219/71‑2 (MQ=255)
gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATc > 1:3632204/1‑48 (MQ=255)
gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATc > 1:3127551/1‑48 (MQ=255)
gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACgt < 1:1029988/71‑1 (MQ=255)
aTTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTg < 1:289853/71‑1 (MQ=255)
tttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGcccc < 1:1098720/59‑1 (MQ=255)
ttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAg > 1:4553732/1‑54 (MQ=255)
tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCg < 1:482126/70‑1 (MQ=255)
|
CTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCG > W3110S.gb/3332545‑3332684
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
