Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,332,615 |
T→A |
intergenic (‑25/+102) |
yhbE ← / ← rpmA |
conserved inner membrane protein/50S ribosomal subunit protein L27 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,332,615 | 0 | T | A | 100.0%
| 76.2
/ NA
| 24 | intergenic (‑25/+102) | yhbE/rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (12/12); total (12/12) |
AATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCC > W3110S.gb/3332564‑3332670
|
aatgccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAAc < 1:3856195/62‑1 (MQ=255)
ctTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTa > 1:2374848/1‑62 (MQ=255)
ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:3947885/1‑62 (MQ=255)
ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:2058345/1‑62 (MQ=255)
ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:2169864/1‑62 (MQ=255)
ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:2294650/1‑62 (MQ=255)
ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:1370048/1‑62 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:2978202/48‑1 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:99987/48‑1 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:453081/48‑1 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:427402/48‑1 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:325759/48‑1 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1948865/48‑1 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1791797/48‑1 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1791416/48‑1 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1538077/48‑1 (MQ=255)
gCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:1468236/48‑1 (MQ=255)
gCAAAGCACATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCgg < 1:4637639/48‑1 (MQ=38)
cAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa > 1:3591697/1‑62 (MQ=255)
cAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa > 1:550538/1‑62 (MQ=255)
cAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa > 1:62211/1‑62 (MQ=255)
gcATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGccc > 1:2341281/1‑61 (MQ=255)
gcATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGccc > 1:2021062/1‑61 (MQ=255)
gcATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGccc > 1:394937/1‑61 (MQ=255)
|
AATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCC > W3110S.gb/3332564‑3332670
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
