Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,739,327 |
C→T |
8.1% |
V195I (GTT→ATT) |
yieH ← |
predicted hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,739,327 | 0 | C | T | 8.1%
| 273.1
/ 10.7
| 86 | V195I (GTT→ATT) | yieH | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (41/38); new base T (2/5); total (43/43) |
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01 |
CCAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATTGT > W3110S.gb/3739259‑3739394
|
ccAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACg < 1:2324548/70‑1 (MQ=255)
ccAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACg < 1:4066550/70‑1 (MQ=255)
ccAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGa > 1:2299128/1‑71 (MQ=255)
aCCACGCGCTTTCCACAGTTCATGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCg < 1:1614848/71‑1 (MQ=255)
aCCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCg < 1:2673052/71‑1 (MQ=255)
aCGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCt > 1:1909796/1‑71 (MQ=255)
gcgcTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTa < 1:2808983/71‑1 (MQ=255)
cgcTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTAt > 1:2449091/1‑71 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGc < 1:12400/65‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATtg < 1:4116304/71‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATtg > 1:2241519/1‑71 (MQ=255)
ttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATtgt > 1:523770/1‑70 (MQ=255)
ttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGATTATtgt > 1:2977812/1‑70 (MQ=255)
tCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAGCGAt > 1:1049049/1‑58 (MQ=255)
tCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGAt > 1:1121660/1‑58 (MQ=255)
ccACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGc < 1:3275537/70‑1 (MQ=255)
caGTTGAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGt < 1:4334363/71‑1 (MQ=255)
caGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGAt > 1:2931608/1‑54 (MQ=255)
caGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATc > 1:3445892/1‑55 (MQ=255)
caGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGt > 1:3360851/1‑71 (MQ=255)
caGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGt < 1:1553929/71‑1 (MQ=255)
caGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGt < 1:1032745/71‑1 (MQ=255)
aGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTc > 1:403609/1‑71 (MQ=255)
gTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCg < 1:4172517/71‑1 (MQ=255)
gTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCg < 1:352902/71‑1 (MQ=255)
ttCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCgg > 1:3581553/1‑71 (MQ=255)
cAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGgcg > 1:161379/1‑71 (MQ=255)
cAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGgcg > 1:3225064/1‑71 (MQ=255)
aGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGa < 1:1708390/47‑1 (MQ=255)
gTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGgcg > 1:3054541/1‑68 (MQ=255)
aCTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAg > 1:2320950/1‑68 (MQ=255)
aCTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGaa < 1:3449386/70‑1 (MQ=255)
tGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCtt < 1:341405/47‑1 (MQ=255)
cGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAg > 1:2777241/1‑67 (MQ=255)
cGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGc > 1:1710413/1‑43 (MQ=255)
cGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGc > 1:1085144/1‑43 (MQ=255)
cGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt > 1:182736/1‑68 (MQ=255)
cGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTa < 1:993431/69‑1 (MQ=255)
cGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg > 1:806259/1‑70 (MQ=255)
gAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa < 1:2490934/71‑1 (MQ=255)
gATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt < 1:1636012/71‑1 (MQ=255)
aTGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACtt > 1:970375/1‑71 (MQ=255)
aTGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACtt < 1:3934194/71‑1 (MQ=255)
tGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc > 1:3175794/1‑71 (MQ=255)
tGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc > 1:3181313/1‑71 (MQ=255)
ggTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACtt < 1:2458669/68‑1 (MQ=255)
gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCa < 1:972416/70‑1 (MQ=255)
gTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCAt < 1:3330383/71‑1 (MQ=255)
tAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCAt < 1:3449755/70‑1 (MQ=255)
ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGGCGGCGCAGAAGTAGAACACTTCCATACCTg < 1:1201073/71‑1 (MQ=255)
gtggtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGc < 1:2787785/71‑1 (MQ=255)
ggtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCAt < 1:2108758/63‑1 (MQ=255)
ggtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCAt > 1:762872/1‑63 (MQ=255)
ggtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGt > 1:3605533/1‑71 (MQ=255)
gtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg < 1:2890720/51‑1 (MQ=255)
gtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTg < 1:3064265/67‑1 (MQ=255)
tgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg < 1:3966954/50‑1 (MQ=255)
tgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTc > 1:670186/1‑70 (MQ=255)
tgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTc > 1:2975815/1‑70 (MQ=255)
tgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCg < 1:902477/71‑1 (MQ=255)
gACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa > 1:3528840/1‑51 (MQ=255)
gACTTTCGGGTGAACGATCGGCTAATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGa > 1:3030935/1‑71 (MQ=255)
aCTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa > 1:4197121/1‑50 (MQ=255)
cTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACtt < 1:488866/54‑1 (MQ=255)
cTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACtt < 1:1608992/54‑1 (MQ=255)
cTTTCGGGTGAACGATCTGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATa > 1:1967572/1‑71 (MQ=255)
cTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGAt > 1:3419682/1‑70 (MQ=255)
tttCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc < 1:3036458/54‑1 (MQ=255)
ttCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg < 1:3397608/45‑1 (MQ=255)
tCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATAc > 1:4299961/1‑69 (MQ=255)
tCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATAc > 1:3421164/1‑69 (MQ=255)
cGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt > 1:309931/1‑41 (MQ=255)
cGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt > 1:575626/1‑41 (MQ=255)
cGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATAc > 1:4108503/1‑68 (MQ=255)
gggTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGa < 1:2711334/71‑1 (MQ=255)
ggTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGa < 1:786392/70‑1 (MQ=255)
ggTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGAt < 1:3755442/71‑1 (MQ=255)
ggTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGGCGATACCAGa < 1:2268402/70‑1 (MQ=255)
tGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGAt < 1:3112036/69‑1 (MQ=255)
tGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATtg > 1:4000101/1‑71 (MQ=255)
gAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt > 1:3793357/1‑36 (MQ=255)
gAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTcc > 1:305184/1‑47 (MQ=255)
aaCGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg < 1:1020322/37‑1 (MQ=255)
aaCGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATtgt < 1:4191809/70‑1 (MQ=255)
aaCGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATtg > 1:3980825/1‑69 (MQ=255)
aCGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac < 1:4158870/41‑1 (MQ=255)
|
CCAACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATACCAGATTGT > W3110S.gb/3739259‑3739394
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
