Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R2 164 48.3 4710601 85.3% 4018142 55.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,739,327 C→T V195I (GTT→ATT)  yieH ← predicted hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,739,3270CT100.0% 449.0 / NA 134V195I (GTT→ATT) yieHpredicted hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (60/74);  total (60/74)

Only 100 of 134 total aligned reads displayed.

CTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCG  >  W3110S.gb/3739270‑3739379
                                                         |                                                    
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:214050/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:3582479/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:3515620/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1106261/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:808760/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:4153485/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1835064/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1808/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:667472/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:2395002/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1575251/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:1495044/62‑1 (MQ=255)
cTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:248799/62‑1 (MQ=255)
 tttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:3067575/61‑1 (MQ=255)
 tttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATc                                                  <  1:79627/61‑1 (MQ=255)
 tttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCg                                                 >  1:3438129/1‑62 (MQ=255)
 tttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGGGGTGACTTTCGGGTGAATGATc                                                  <  1:680987/61‑1 (MQ=255)
  ttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCgg                                                <  1:3069057/62‑1 (MQ=255)
  ttCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCgg                                                <  1:1307616/62‑1 (MQ=255)
                   cTGCGAAAGATGGGTAAAGGTGGTGACTTGCGGGTGAATGATCGGCTTATTGTGCGGGTCgg                               >  1:210127/1‑62 (MQ=255)
                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTAt                                           >  1:485031/1‑45 (MQ=255)
                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                            >  1:4440142/1‑60 (MQ=255)
                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                            >  1:848003/1‑60 (MQ=255)
                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                            >  1:3958167/1‑60 (MQ=255)
                        aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                            >  1:897481/1‑60 (MQ=255)
                         aaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:2215558/1‑62 (MQ=255)
                           gATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:3566267/1‑60 (MQ=255)
                           gATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:1032803/1‑60 (MQ=255)
                           gATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:1978080/1‑60 (MQ=255)
                           gATGGGTAAAGGTGATGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGa                         >  1:3232205/1‑60 (MQ=255)
                               ggTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGa                   <  1:4400057/62‑1 (MQ=255)
                               ggTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGa                   <  1:2434117/62‑1 (MQ=255)
                               ggTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGa                   <  1:3024172/62‑1 (MQ=255)
                               ggTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGa                   <  1:2976506/62‑1 (MQ=255)
                                gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt                      <  1:1705645/58‑1 (MQ=255)
                                gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt                      <  1:2498525/58‑1 (MQ=255)
                                gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt                      <  1:1483242/58‑1 (MQ=255)
                                gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGt                      <  1:2722087/58‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:3648402/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:4109575/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:426315/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:3104013/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:4445698/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:965493/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:2119469/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:2577889/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:1774110/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:1482469/62‑1 (MQ=255)
                                  aaaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                <  1:1985580/62‑1 (MQ=255)
                                    aGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:3247866/1‑62 (MQ=255)
                                    aGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:3479898/1‑62 (MQ=255)
                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATtgtg                                       >  1:3712250/1‑36 (MQ=255)
                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:426408/1‑61 (MQ=255)
                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:774167/1‑61 (MQ=255)
                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:3173004/1‑61 (MQ=255)
                                     ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt              >  1:3511857/1‑61 (MQ=255)
                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:3374194/1‑58 (MQ=255)
                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:689723/1‑58 (MQ=255)
                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:375932/1‑58 (MQ=255)
                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:622382/1‑58 (MQ=255)
                                       tggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               >  1:2260877/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:1154107/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:2734052/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:2088849/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:4162358/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:1680093/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:4516161/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:1500924/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:218394/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:2212638/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:112656/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:2408080/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:3099338/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:3093250/1‑58 (MQ=255)
                                          tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc            >  1:104295/1‑58 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTTTTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:2177909/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGTGGGTCGGCGCAGAAGTAGAacac               <  1:838174/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:1254524/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:1464711/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:4003167/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:524869/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:963341/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:1010249/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:2614717/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:4209116/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:1942403/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:2564764/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:3593582/54‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc       <  1:1502552/62‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc       <  1:2840029/62‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc       <  1:3422255/62‑1 (MQ=255)
                                           gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc       <  1:4502961/62‑1 (MQ=255)
                                              tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAacac               <  1:2687016/51‑1 (MQ=255)
                                              tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt     <  1:4517833/61‑1 (MQ=255)
                                                tCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGc   >  1:1079501/1‑61 (MQ=255)
                                                tCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  >  1:2141692/1‑62 (MQ=255)
                                                tCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  >  1:371782/1‑62 (MQ=255)
                                                tCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  >  1:4033634/1‑62 (MQ=255)
                                                  gggTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  <  1:3626164/60‑1 (MQ=255)
                                                  gggTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCg  <  1:3631534/60‑1 (MQ=255)
                                                         |                                                    
CTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCG  >  W3110S.gb/3739270‑3739379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A