Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F17 I0 R2	162	60.6	4616657	98.5%	4547407	62.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,586,637	G→A	14.3%	P365L (CCG→CTG)	hsdM ←	DNA methylase M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,586,637	0	G	A	14.3%	191.2 / 20.1	70	P365L (CCG→CTG)	hsdM	DNA methylase M
Reads supporting (aligned to +/- strand): ref base G (27/33); new base A (4/6); total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

GCCGAAACTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTAAAAAATACCG  >  W3110S.gb/4586567‑4586705

gCCGAAACTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGAATCg                                                                      >  1:3274249/1‑71 (MQ=255)

 ccGAATCTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCgg                                                                     <  1:4525880/71‑1 (MQ=255)

 ccGAAACTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCg                                                                      <  1:1045263/70‑1 (MQ=255)

  cGAAACTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCgg                                                                     >  1:3612849/1‑70 (MQ=255)

   gAAACTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGTTTCGGGt                                                                   <  1:839386/71‑1 (MQ=255)

    aaaCTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGtt                                                                  <  1:1587825/71‑1 (MQ=255)

    aaaCTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGtt                                                                  >  1:1551108/1‑71 (MQ=255)

    aaaCTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCAGGtt                                                                  >  1:4349585/1‑71 (MQ=255)

          ggCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGcc                                                              <  1:1074727/69‑1 (MQ=255)

            cATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCgg                                                                     >  1:2051794/1‑60 (MQ=255)

               atTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTcc                                                       >  1:1824533/1‑71 (MQ=255)

                 tGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCggg                                                                    >  1:2983241/1‑56 (MQ=255)

                 tGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCggg                                                                    >  1:3961565/1‑56 (MQ=255)

                 tGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCAGCGTccc                                                      <  1:4267150/70‑1 (MQ=255)

                 tGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGt                                                         <  1:4566061/67‑1 (MQ=255)

                 tGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCt                                                     >  1:1045606/1‑71 (MQ=255)

                  ggTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCt                                                     <  1:1345941/70‑1 (MQ=255)

                  ggTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCt                                                     <  1:1354987/70‑1 (MQ=255)

                   gTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCttt                                                   >  1:2844762/1‑71 (MQ=255)

                   gTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCtt                                                    <  1:4255832/70‑1 (MQ=255)

                   gTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCtt                                                    <  1:2741607/70‑1 (MQ=255)

                     aCGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCggg                                                                    <  1:1373643/52‑1 (MQ=255)

                      cGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTgg                                                 >  1:74933/1‑70 (MQ=255)

                         aGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTaaa                                             <  1:4101916/71‑1 (MQ=255)

                           gTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGtt                                                                  <  1:64637/48‑1 (MQ=255)

                            tCATACACCCACACATCATCGGTACAGTTCTTATCCTTATTCGGGTTCGCCACCGTCCCTTTGGTAAAGaa                                          <  1:3667588/71‑1 (MQ=255)

                            tCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGaa                                          <  1:2965523/71‑1 (MQ=255)

                              aTACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCAc                                                            >  1:717490/1‑51 (MQ=255)

                              aTACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGaa                                          >  1:1708185/1‑69 (MQ=255)

                               tACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGc                                                               <  1:753303/47‑1 (MQ=255)

                                acacCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCtt                                                    >  1:4172121/1‑57 (MQ=255)

                                 cacCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCggc                                                              <  1:3787781/46‑3 (MQ=255)

                                 cacCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGaa                                          >  1:3235056/1‑66 (MQ=255)

                                  acCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTc                                                                 >  1:935022/1‑42 (MQ=255)

                                  acCCACACATCATCGGTACAGTTCTTATCCTGATTCAGGTTCGCCAcc                                                           >  1:4096890/1‑48 (MQ=255)

                                  acCCACACATCATCGGTACAGTTCTTAACCTGATTCGGGTTCGCCa                                                             >  1:2248124/1‑46 (MQ=255)

                                    ccACACATCATCGGTACAGTTCTTATCCTGATTCgc                                                                     >  1:2467801/1‑35 (MQ=255)

                                    ccACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGt                                  <  1:2885498/71‑1 (MQ=255)

                                    ccACACATCATCGGTACAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGt                                  <  1:746677/71‑1 (MQ=255)

                                    ccACACATCATCGGTACAGTTCTTATACTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACa                                        >  1:3756641/1‑65 (MQ=255)

                                     cacacaTCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCa                                                             >  1:4535044/1‑43 (MQ=255)

                                      acacaTCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCa                                                             >  1:3581979/1‑42 (MQ=255)

                                      acacaTCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTaa                                              <  1:570976/57‑1 (MQ=255)

                                      acacaTCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACa                                        >  1:3335965/1‑63 (MQ=255)

                                      acacaTCATCGGTACAGTTCTTATCCTGATTCGGGTACGCCACCGTCCCTTTGGTAAAGAACAGCACg                                   >  1:3884243/1‑68 (MQ=255)

                                      acacaTCATCGGTACAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTg                                >  1:1903466/1‑71 (MQ=255)

                                       cacaTCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTgg                               <  1:161101/71‑1 (MQ=255)

                                        acaTCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGt                              <  1:3336889/71‑1 (MQ=255)

                                         catcatCGGTACAGTTCTTATCCTGATTCGGGTTc                                                                 >  1:3217258/1‑35 (MQ=255)

                                         catcatCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTc                             <  1:2656420/71‑1 (MQ=255)

                                          atcatcGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCt                            <  1:1386693/71‑1 (MQ=255)

                                          atcatcGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCt                            <  1:1582270/71‑1 (MQ=255)

                                          atcatcGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCt                            <  1:1587617/71‑1 (MQ=255)

                                          atcatcGGTACAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTg                                <  1:2243010/67‑1 (MQ=255)

                                           tcatcGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTaaa                                             >  1:2079748/1‑53 (MQ=255)

                                           tcatcGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCtt                           >  1:1545450/1‑71 (MQ=255)

                                            catcGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTaa                                              >  1:45779/1‑51 (MQ=255)

                                             atcGGTACAGTTCTTATCCTGATTCGGGTTCGCCAc                                                            >  1:4262767/1‑36 (MQ=255)

                                             atcGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCa                         >  1:1284452/1‑71 (MQ=255)

                                             atcGGTACAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCa                         >  1:299202/1‑71 (MQ=255)

                                              tcGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCAc                        <  1:4500749/71‑1 (MQ=255)

                                               cGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAg                                            >  1:397909/1‑50 (MQ=255)

                                                ggTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACg                       <  1:2054502/70‑1 (MQ=255)

                                                 gTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAg                                       >  1:2113645/1‑53 (MQ=255)

                                                  tACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACg                       <  1:3550033/68‑1 (MQ=255)

                                                    cAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCt                   <  1:3317148/70‑1 (MQ=255)

                                                    cAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCt                   <  1:3680226/70‑1 (MQ=255)

                                                    cAGTTCTTATCCCGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCt                   >  1:1169085/1‑70 (MQ=255)

                                                      gTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGTTAAAGAACAGCACGt                                  <  1:1321738/53‑1 (MQ=255)

                                                       ttCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCt                   <  1:423335/67‑1 (MQ=255)

                                                         cTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCa                         >  1:2992385/1‑59 (MQ=255)

                                                          ttATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAg                                            <  1:3850954/39‑1 (MQ=255)

                                                            aTCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAg                                       <  1:1575834/42‑1 (MQ=255)

                                                            aTCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTg                                <  1:92804/49‑1 (MQ=255)

                                                                 gATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTAAAAAATa     >  1:1345569/1‑71 (MQ=255)

                                                                  aTTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTAAAAAATa     <  1:1786139/70‑1 (MQ=255)

                                                                  aTTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGAAAAAAAt      <  1:451709/69‑1 (MQ=255)

                                                                  aTTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGGCTTCACGCCCTGAGCGTAAAAAAt      <  1:693740/69‑1 (MQ=255)

                                                                     cGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTAAAAAATACCg  <  1:2672324/70‑1 (MQ=255)

GCCGAAACTCGGCATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTAAAAAATACCG  >  W3110S.gb/4586567‑4586705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A