Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,655,312 |
C→A |
L106L (CTG→CTT) |
rspB ← |
predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,655,312 | 0 | C | A | 100.0%
| 16.9
/ NA
| 8 | L106L (CTG→CTT) | rspB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/8); total (0/8) |
CGGCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCCAGTG > W3110S.gb/1655266‑1655316
|
gagCATATTCACTGAAACCACCGTCAGCTTGCACACCTAATACAGCAAgtg < 1:869469/49‑1 (MQ=255)
cagCATATTCTCTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg < 1:1309087/49‑1 (MQ=255)
cagCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg < 1:1619055/49‑1 (MQ=255)
cagCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg < 1:327359/49‑1 (MQ=255)
cagCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg < 1:733408/49‑1 (MQ=255)
cagCACATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg < 1:545809/49‑1 (MQ=255)
atatTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg < 1:1256162/47‑1 (MQ=255)
cTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg < 1:1012846/40‑1 (MQ=255)
|
CGGCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCCAGTG > W3110S.gb/1655266‑1655316
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
