Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,609,702 |
C→T |
E151E (GAG→GAA) |
fre ← |
flavin reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,609,702 | 0 | C | T | 100.0%
| 43.0
/ NA
| 17 | E151E (GAG→GAA) | fre | flavin reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/17); total (0/17) |
GACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCA > W3110S.gb/3609671‑3609732
|
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:532245/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:987338/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:95917/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:947983/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:83575/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:70641/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:702167/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:641651/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:533871/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:1017824/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:528798/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:470150/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:1476863/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:1312667/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:1259112/62‑1 (MQ=255)
gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:1250762/62‑1 (MQ=255)
gACCAGGATGCTT‑AACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa < 1:133992/61‑1 (MQ=255)
|
GACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCA > W3110S.gb/3609671‑3609732
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
