Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R1
|
87 |
56.5 |
4283937 |
98.6% |
4223961 |
62.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,497,165 |
G→A |
10.8% |
A65T (GCG→ACG) |
fsaB → |
fructose‑6‑phosphate aldolase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,497,165 | 0 | G | A | 10.8%
| 136.5
/ 5.4
| 46 | A65T (GCG→ACG) | fsaB | fructose‑6‑phosphate aldolase 2 |
| Reads supporting (aligned to +/- strand): ref base G (23/18); new base A (3/2); total (26/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGTGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGAAAATCCCGGT > W3110S.gb/3497095‑3497235
|
aGTGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcg > 1:1089710/1‑71 (MQ=255)
aGTGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcg > 1:3381667/1‑71 (MQ=255)
gctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgc > 1:3801753/1‑69 (MQ=255)
ctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgcg > 1:3236935/1‑69 (MQ=255)
ctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACgcgc < 1:880556/70‑1 (MQ=255)
ccGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAggg > 1:1426321/1‑71 (MQ=255)
ccGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAgg < 1:2321030/70‑1 (MQ=255)
gTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgcg > 1:318640/1‑62 (MQ=255)
tGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtggtg > 1:1895175/1‑71 (MQ=255)
aaaaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCATGGGAtggtgg < 1:70825/69‑1 (MQ=255)
aaaaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtggtgg < 1:3810924/69‑1 (MQ=255)
aaaaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaa < 1:2317414/71‑1 (MQ=255)
aaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAgg < 1:1147766/58‑1 (MQ=255)
aaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtg < 1:1652981/63‑1 (MQ=255)
aaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaaga < 1:3269085/71‑1 (MQ=255)
aGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaagaa > 1:1831150/1‑70 (MQ=255)
gCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgcg < 1:1841436/51‑1 (MQ=255)
gCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagc < 1:3144252/71‑1 (MQ=255)
aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgc < 1:3110117/47‑1 (MQ=255)
aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgc < 1:1012020/47‑1 (MQ=255)
aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgc < 1:3606348/47‑1 (MQ=255)
ttGGTTATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagc < 1:122841/67‑1 (MQ=255)
ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaag > 1:4075628/1‑71 (MQ=255)
ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaag > 1:2821485/1‑71 (MQ=255)
gTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaa < 1:1590649/67‑1 (MQ=255)
gatgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCt > 1:2089674/1‑71 (MQ=255)
atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTg > 1:755376/1‑71 (MQ=255)
atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTg > 1:571212/1‑71 (MQ=255)
tgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgc > 1:1402213/1‑71 (MQ=255)
tgggCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGTGAtggtgg < 1:1431051/50‑1 (MQ=255)
aGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtggtg > 1:425707/1‑50 (MQ=255)
aGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaag > 1:429334/1‑54 (MQ=255)
aGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGCAGAAGCGAAGCGCCTgcgc < 1:3502646/71‑1 (MQ=255)
gggCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGcgcg < 1:2463085/71‑1 (MQ=255)
aTTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGc < 1:2327922/71‑1 (MQ=255)
ttCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGc > 1:2922103/1‑70 (MQ=255)
cTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAgcgc > 1:2576805/1‑57 (MQ=255)
tGTTTTCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTAtt > 1:3138620/1‑71 (MQ=255)
tGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTAtt > 1:1186928/1‑71 (MQ=255)
tGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTAt > 1:3153153/1‑70 (MQ=255)
cTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGga < 1:2392634/37‑1 (MQ=255)
cAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc < 1:3168681/54‑1 (MQ=255)
aGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTgcgc > 1:3164945/1‑53 (MQ=255)
aGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTAtt > 1:2995690/1‑71 (MQ=255)
aGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTAtt < 1:3943004/71‑1 (MQ=255)
gACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaag > 1:4172383/1‑43 (MQ=255)
ccATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATtgtg > 1:3455454/1‑71 (MQ=255)
ccATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATtgt < 1:943613/70‑1 (MQ=255)
cATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaagccc > 1:4033642/1‑41 (MQ=255)
aTGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcg < 1:1395996/36‑1 (MQ=255)
tGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTgtggt > 1:20430/1‑70 (MQ=255)
tGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTgtggt > 1:1141318/1‑70 (MQ=255)
tGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGt > 1:2866291/1‑62 (MQ=255)
tGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTgtggt > 1:1031164/1‑70 (MQ=255)
cgcgACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATtgtg < 1:1436635/63‑1 (MQ=255)
gcgACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGa > 1:425226/1‑66 (MQ=255)
gcgcAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGAAAATCCCGGt < 1:2104332/71‑1 (MQ=255)
|
AGTGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGAAAATCCCGGT > W3110S.gb/3497095‑3497235
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
