breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 70,434 C→T 47.8% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator
RA 70,581 C→T 38.7% V65V (GTC→GTT araC → DNA‑binding transcriptional dual regulator
RA 70,740 T→G 45.5% G118G (GGT→GGG araC → DNA‑binding transcriptional dual regulator
RA 71,079 C→T 47.9% R231R (CGC→CGT araC → DNA‑binding transcriptional dual regulator
RA 71,082 T→C 46.3% I232I (ATT→ATC araC → DNA‑binding transcriptional dual regulator
RA 71,085 T→C 42.7% S233S (AGT→AGC araC → DNA‑binding transcriptional dual regulator
RA 71,109 T→C 45.9% T241T (ACT→ACC araC → DNA‑binding transcriptional dual regulator
RA 71,175 A→G 42.2% R263R (CGA→CGG araC → DNA‑binding transcriptional dual regulator
RA 71,214 T→C 51.7% F276F (TTT→TTC araC → DNA‑binding transcriptional dual regulator
RA 155,463 A→T 100% *247K (TAA→AAA)  ecpD ← predicted periplasmic pilin chaperone
RA 236,101 A→C 53.5% D12A (GAT→GCT)  dnaQ → DNA polymerase III epsilon subunit
RA 236,107 A→C 52.7% E14A (GAA→GCA)  dnaQ → DNA polymerase III epsilon subunit
RA 258,840 A→G 6.7% V162A (GTT→GCT)  phoE ← outer membrane phosphoporin protein E
RA 267,502 G→T 7.7% P228H (CCT→CAT)  ykfA ← predicted GTP‑binding protein
RA 345,099 C→T 9.6% A155T (GCC→ACC)  yahN ← neutral amino‑acid efflux system
RA 349,140 C→T 11.1% intergenic (+344/‑96) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 429,850 T→C 12.8% Y8H (TAT→CAT)  yajD → conserved hypothetical protein
RA 478,343 C→T 8.3% A45T (GCT→ACT)  ylaC ← predicted inner membrane protein
RA 547,694 A→G 100% pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 547,831:1 +G 100% pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 647,863 A→G 9.1% M311T (ATG→ACG)  citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit
MC JC 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]
RA 1,008,134 C→T 15.1% intergenic (‑112/‑132) ycbX ← / → ycbY predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase
RA 1,036,407 A→G 10.2% E72E (GAA→GAG hyaD → protein involved in processing of HyaA and HyaB proteins
RA 1,084,947 A→G 16.9% K384E (AAA→GAA)  ycdB → conserved hypothetical protein
RA 1,093,686 T→C 100% V130A (GTA→GCA)  ycdT → predicted diguanylate cyclase
RA 1,115,786 T→C 8.1% intergenic (‑28/+55) msyB ← / ← mdtG hypothetical protein/predicted drug efflux system
RA 1,224,919 G→A 9.4% intergenic (‑423/‑123) ymgG ← / → ymgH hypothetical protein/hypothetical protein
RA 1,255,918 C→T 10.9% G538S (GGC→AGC)  ycgV ← predicted adhesin
RA 1,295,635 T→C 7.9% Y178H (TAC→CAC)  tdk → thymidine kinase/deoxyuridine kinase
MC JC 1,301,041 Δ1,336 bp 100% insCinsD insC, insD
RA 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit
RA 1,303,633 G→T 100% Q246Y (CAG→TAT oppA → oligopeptide transporter subunit
RA 1,516,711 C→T 17.2% A79V (GCG→GTG)  ydcV → predicted spermidine/putrescine transporter subunit
RA 1,819,760 G→T 10.8% A152E (GCG→GAG)  chbF ← cryptic phospho‑beta‑glucosidase, NAD(P)‑binding
RA 1,872,347 A→G 7.7% K83K (AAA→AAG yeaI → predicted diguanylate cyclase
RA 1,989,353 T→A 6.3% S98T (TCT→ACT)  yecI → predicted ferritin‑like protein
RA 1,989,358 T→A 5.9% S99R (AGT→AGA yecI → predicted ferritin‑like protein
RA 2,170,757 T→C 8.0% intergenic (‑314/‑92) yegR ← / → yegS hypothetical protein/conserved hypothetical protein
MC JC 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]
MC JC 2,320,795 Δ1,336 bp 100% insD[rcsC] insD, insC, [rcsC]
RA 2,809,581 G→A 54.7% G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator
RA 2,855,731 G→T 23.3% intergenic (‑269/‑18) ygbA ← / → mutS hypothetical protein/methyl‑directed mismatch repair protein
RA 2,855,732 G→A 23.3% intergenic (‑270/‑17) ygbA ← / → mutS hypothetical protein/methyl‑directed mismatch repair protein
RA 2,866,110 T→A 100% intergenic (‑20/+160) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein
RA 3,001,511 T→C 5.5% I81T (ATA→ACA)  xdhB → xanthine dehydrogenase, FAD‑binding subunit
RA 3,139,938 C→T 6.4% R2H (CGT→CAT)  hybD ← predicted maturation element for hydrogenase 2
RA 3,161,757 G→A 8.5% T127T (ACC→ACT plsC ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
MC JC 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]
RA 3,294,438 T→C 11.9% L395S (TTG→TCG)  yraM → conserved hypothetical protein
RA 3,306,434 G→A 9.6% L428L (CTG→TTG)  deaD ← ATP‑dependent RNA helicase
RA 3,332,615 T→A 10.2% intergenic (‑25/+102) yhbE ← / ← rpmA conserved inner membrane protein/50S ribosomal subunit protein L27
RA 3,459,280 T→C 10.2% E15G (GAA→GGA)  secE ← preprotein translocase membrane subunit
RA 3,497,165 G→A 10.8% A65T (GCG→ACG)  fsaB → fructose‑6‑phosphate aldolase 2
RA 3,516,285 T→C 6.9% intergenic (+20/‑47) hslU → / → menA molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase
RA 3,639,730 T→C 10.6% K222R (AAA→AGA)  xerC ← site‑specific tyrosine recombinase
RA 3,727,747 T→C 11.9% V168V (GTT→GTC pstA → phosphate transporter subunit
RA 3,739,327 C→T 10.5% V195I (GTT→ATT)  yieH ← predicted hydrolase
MC JC 3,742,938 Δ1,336 bp 100% insDinsC insD, insC
MC JC 3,750,593 Δ1,199 bp 100% insH insH
RA 3,824,280 T→C 100% pseudogene (413/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,326 T→C 100% pseudogene (459/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,362 A→T 100% pseudogene (495/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,374 C→T 100% pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,380 2 bp→AA 100% pseudogene (513‑514/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,410 T→A 100% pseudogene (543/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,514 T→C 100% pseudogene (647/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,524 T→G 100% pseudogene (657/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,533:1 +G 100% pseudogene (666/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,832,784 T→C 7.3% C156R (TGC→CGC)  rfaQ → lipopolysaccharide core biosynthesis protein
RA 3,836,867 A→G 8.7% K206K (AAA→AAG rfaB → UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
RA 3,886,994 G→A 5.5% S226N (AGC→AAC)  yiaV → membrane fusion protein (MFP) component of efflux pump, signal anchor
RA 3,915,028 C→T 6.7% intergenic (+24/‑71) ysaB → / → glyQ hypothetical protein/glycine tRNA synthetase, alpha subunit
RA 3,915,241 A→G 15.0% E48G (GAG→GGG)  glyQ → glycine tRNA synthetase, alpha subunit
RA 4,012,376 G→A 12.7% A500T (GCC→ACC)  rbbA → fused ribosome‑associated ATPases
RA 4,164,007 C→A 7.6% T10T (ACC→ACA yheO → predicted DNA‑binding transcriptional regulator
RA 4,218,735 G→T 8.5% W288C (TGG→TGT metA → homoserine transsuccinylase
MC JC 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]
RA 4,350,232 G→A 8.3% R3C (CGC→TGC)  yjdF ← conserved inner membrane protein
RA 4,371,273 Δ2 bp 100% intergenic (‑6/+299) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,477,260 G→A 12.1% intergenic (‑45/+236) pyrL ← / ← yjgH pyrBI operon leader peptide/predicted mRNA endoribonuclease
RA 4,544,435 A→G 10.8% intergenic (‑252/‑1204) yjhA ← / → fimB N‑acetylnuraminic acid outer membrane channel protein/tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,563,589 T→A 100% K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase
RA 4,580,803 C→T 16.7% pseudogene (1987/2721 nt) yjiV → ECK4334:JW5954:b4486; conserved hypothetical protein
RA 4,606,089 G→A 11.1% N37N (AAC→AAT dnaT ← DNA biosynthesis protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 573850–575008 575999 992–2150 12 [10] [0] 113 insH–[nmpC] insH,[nmpC]
* * ÷ W3110S.gb 1328297 1328351 55 11 [9] [10] 13 yciQ predicted inner membrane protein
* * ÷ W3110S.gb 1828078 1828135 58 11 [10] [8] 11 astE succinylglutamate desuccinylase
* * ÷ W3110S.gb 2041772 2041817 46 12 [8] [10] 11 yedY predicted reductase
* * ÷ W3110S.gb 2569105 2569138 34 13 [10] [9] 11 eutE predicted aldehyde dehydrogenase, ethanolamine utilization protein
* * ÷ W3110S.gb 2827382 2827428 47 11 [10] [10] 12 gutM DNA‑binding transcriptional activator
* * ÷ W3110S.gb 4213629–4215889 4216717–4215891 3–3089 11 [8] [10] 11 rrlE–[rrfE] rrlE,[rrfE]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 70061 =47 (1.020)39 (1.080)
+CGCGTACAACT
27/80 NT 45.0% intergenic (‑13/‑326) araB/araC L‑ribulokinase/DNA‑binding transcriptional dual regulator
?W3110S.gb 236044 = 75 (1.620)intergenic (‑42/‑23) rnhA/dnaQ ribonuclease HI, degrades RNA of DNA‑RNA hybrids/DNA polymerase III epsilon subunit
* ? W3110S.gb = 7126545 (0.970)12 (0.360)
+AGGGTCCCTAAGTC
10/74 NT 36.9% coding (879/879 nt) araC DNA‑binding transcriptional dual regulator
?W3110S.gb 3464879 = 12 (0.260)intergenic (‑255/+46) murB/rrfB UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding/5S ribosomal RNA
* ? W3110S.gb = 23679829 (0.630)10 (0.250)
+TTTAAT
7/90 NT 22.3% coding (732/732 nt) dnaQ DNA polymerase III epsilon subunit
?W3110S.gb = 3142784 50 (1.080)coding (37/1179 nt) hybB predicted hydrogenase 2 cytochrome b type component
* ? W3110S.gb = 274373NA (NA)63 (1.360) 35/102 NT 100% intergenic (‑32/‑176) insH/mmuP IS5 transposase and trans‑activator/predicted S‑methylmethionine transporter
?W3110S.gb 576000 = 0 (0.000)pseudogene (109/1128 nt) nmpC DLP12 prophage region; ECK0544:JW5078:b0553; truncated outer membrane porin
* ? W3110S.gb = 314744NA (NA)6 (0.150) 4/88 NT NA coding (239/309 nt) insE IS3 element protein
?W3110S.gb = 314761 NA (NA)coding (256/309 nt) insE IS3 element protein
* ? W3110S.gb = 314796NA (NA)3 (0.080) 3/82 NT NA coding (291/309 nt) insE IS3 element protein
?W3110S.gb = 314812 NA (NA)coding (307/309 nt)
coding (2/867 nt)
insE
insF
IS3 element protein
IS3 element protein InsF
* ? W3110S.gb = 315707NA (NA)33 (0.710) 26/102 NT 100% coding (654/687 nt) ykgA predicted DNA‑binding transcriptional regulator
?W3110S.gb 576911 = 0 (0.000)coding (76/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 566000 =NA (NA)43 (0.930) 30/102 NT 100% intergenic (+90/‑56) ybcD/insE DLP12 prophage region; ECK0532:JW0527:b4508; predicted replication protein fragment/IS3 element protein InsE
?W3110S.gb = 576913 0 (0.000)coding (78/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 713380 =34 (0.730)27 (0.630) 23/94 NT 46.8% intergenic (‑156/‑29) ybfF/seqA conserved hypothetical protein/regulatory protein for replication initiation
?W3110S.gb = 4125340 30 (0.700)coding (836/837 nt) dam DNA adenine methylase
* ? W3110S.gb = 71395466 (1.430)40 (0.860) 26/102 NT 37.6% coding (546/546 nt) seqA regulatory protein for replication initiation
?W3110S.gb 2809398 = 67 (1.450)intergenic (+63/‑28) ygaH/mprA predicted inner membrane protein/DNA‑binding transcriptional regulator
* ? W3110S.gb 1098035 =33 (0.710)3 (0.070) 3/90 NT 8.6% intergenic (‑142/‑92) serX/ycdW tRNA‑Ser/2‑ketoacid reductase
?W3110S.gb 1098067 = 35 (0.860)intergenic (‑174/‑60) serX/ycdW tRNA‑Ser/2‑ketoacid reductase
* ? W3110S.gb 1209367 =22 (0.480)25 (0.790) 21/70 NT 66.7% coding (290/630 nt) ycfK hypothetical protein
?W3110S.gb 1211196 = 10 (0.320)pseudogene (37/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb = 120938219 (0.410)22 (0.700) 18/70 NT 65.8% coding (305/630 nt) ycfK hypothetical protein
?W3110S.gb = 1211179 10 (0.320)pseudogene (54/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb = 177341854 (1.170)4 (0.100) 3/90 NT 7.4% coding (634/1215 nt) ydiM predicted transporter
?W3110S.gb = 1773431 52 (1.280)coding (647/1215 nt) ydiM predicted transporter
* ? W3110S.gb = 193127944 (0.950)3 (0.070) 3/92 NT 7.2% coding (143/660 nt) yebE conserved hypothetical protein
?W3110S.gb = 1931292 38 (0.910)coding (130/660 nt) yebE conserved hypothetical protein
* ? W3110S.gb 2018571 =11 (0.240)22 (0.510) 19/96 NT 68.0% coding (567/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb 2519723 = NA (NA)intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/predicted transposase
* ? W3110S.gb = 20185833 (0.060)38 (0.860) 28/98 NT 93.0% coding (579/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb = 2521059 NA (NA)intergenic (+170/+30) insL/yfeA predicted transposase/predicted diguanylate cyclase
* ? W3110S.gb 2172308 =NA (NA)72 (1.590) 43/100 NT 100% pseudogene (1563/2041 nt) gatR DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
?W3110S.gb = 3920281 0 (0.000)coding (88/213 nt) cspA major cold shock protein
* ? W3110S.gb 2172309 =NA (NA)50 (1.130) 36/98 NT 100% pseudogene (1562/2041 nt) gatR DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
?W3110S.gb 3932930 = 0 (0.000)coding (219/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 2173564NA (NA)107 (2.360) 58/100 NT 100% pseudogene (307/2041 nt)
pseudogene (307/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb 3920278 = 0 (0.000)coding (91/213 nt) cspA major cold shock protein
* ? W3110S.gb = 2173565NA (NA)42 (0.910) 31/102 NT 100% pseudogene (306/2041 nt)
pseudogene (306/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb = 3932933 0 (0.000)coding (222/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 2412225NA (NA)71 (1.530) 42/102 NT 93.4% intergenic (‑55/‑782) insA/yfbQ IS1 element protein/predicted aminotransferase
?W3110S.gb = 3748807 5 (0.110)coding (176/984 nt) tnaB tryptophan transporter of low affinity
* ? W3110S.gb 2750405 =45 (0.970)4 (0.100) 4/90 NT 9.0% intergenic (+40/‑46) yfjB/recN NAD kinase/recombination and repair protein
?W3110S.gb 2750425 = 41 (1.010)intergenic (+60/‑26) yfjB/recN NAD kinase/recombination and repair protein
* ? W3110S.gb = 285574721 (0.450)17 (0.370) 11/100 NT 31.0% intergenic (‑285/‑2) ygbA/mutS hypothetical protein/methyl‑directed mismatch repair protein
?W3110S.gb 4124505 = 55 (1.210)coding (1/837 nt) dam DNA adenine methylase
* ? W3110S.gb 3028230 =64 (1.380)3 (0.070) 3/94 NT 5.3% coding (1358/1920 nt) ygfT fused predicted oxidoreductase Fe‑S subunit and nucleotide‑binding subunit
?W3110S.gb 3028255 = 49 (1.150)coding (1333/1920 nt) ygfT fused predicted oxidoreductase Fe‑S subunit and nucleotide‑binding subunit
* ? W3110S.gb 3152775 =69 (1.490)3 (0.070) 3/90 NT 5.1% coding (557/660 nt) yghB conserved inner membrane protein
?W3110S.gb 3152797 = 52 (1.280)coding (579/660 nt) yghB conserved inner membrane protein
* ? W3110S.gb = 37469120 (0.000)81 (1.900) 51/94 NT 100% coding (71/447 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 3748112 = 0 (0.000)coding (871/984 nt) tnaB tryptophan transporter of low affinity
* ? W3110S.gb 3748799 =4 (0.090)51 (1.100) 41/102 NT 92.7% coding (184/984 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 4056222 = NA (NA)intergenic (‑564/+15) yrhB/insB hypothetical protein/IS1 transposase InsAB'
* ? W3110S.gb 3998498 =89 (1.920)5 (0.130) 3/88 NT 6.1% coding (1058/1470 nt) yhiP predicted transporter
?W3110S.gb 3998524 = 77 (1.930)coding (1032/1470 nt) yhiP predicted transporter
* ? W3110S.gb 4547364 =29 (0.630)5 (0.130) 4/84 NT 16.7% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb 4547678 = 26 (0.690)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
* ? W3110S.gb = 454737229 (0.630)7 (0.180) 6/84 NT 22.0% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb = 4547668 26 (0.690)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin