Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R1
|
87 |
56.5 |
4283937 |
98.6% |
4223961 |
62.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,012,376 |
G→A |
12.7% |
A500T (GCC→ACC) |
rbbA → |
fused ribosome‑associated ATPases |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,012,376 | 0 | G | A | 12.7%
| 155.1
/ 12.4
| 55 | A500T (GCC→ACC) | rbbA | fused ribosome‑associated ATPases |
| Reads supporting (aligned to +/- strand): ref base G (21/27); new base A (3/4); total (24/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 |
CATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAACGAAGCCGAAG > W3110S.gb/4012306‑4012445
|
cATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAg < 1:2530825/71‑1 (MQ=255)
cACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAgccgc > 1:3596028/1‑70 (MQ=255)
aCTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAgcc < 1:1971975/67‑1 (MQ=255)
aCTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCa < 1:1713591/71‑1 (MQ=255)
aTGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGGACTGGTTGAGAAACGCGGAGCCGCCAGTCt < 1:1462034/71‑1 (MQ=255)
gCCGGAAAAGTGCTTGCCAGAGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGaa > 1:422027/1‑69 (MQ=255)
ccGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGaa > 1:3515421/1‑68 (MQ=255)
ggAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAgg > 1:2321812/1‑70 (MQ=255)
ggAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGc < 1:3296945/71‑1 (MQ=255)
aaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAttt < 1:2420878/71‑1 (MQ=255)
aaGTGCTTGCCAGCGGTACACCGCAGGAACTGGGTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAttt < 1:77182/71‑1 (MQ=255)
gTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTAt < 1:3573356/71‑1 (MQ=255)
tGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATc < 1:2309158/71‑1 (MQ=255)
cTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTg < 1:1471800/54‑1 (MQ=255)
gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGaa < 1:4063298/54‑1 (MQ=255)
gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGcc < 1:2778695/69‑1 (MQ=255)
gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCt < 1:2901557/70‑1 (MQ=255)
gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTa > 1:3500686/1‑71 (MQ=255)
gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTa > 1:2144497/1‑71 (MQ=255)
gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTa > 1:2260360/1‑71 (MQ=255)
ccAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAaga > 1:1997309/1‑55 (MQ=255)
aGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAt < 1:1858668/58‑1 (MQ=255)
gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTg < 1:569528/71‑1 (MQ=255)
gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTg > 1:3880029/1‑71 (MQ=255)
gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTg < 1:1462063/71‑1 (MQ=255)
cGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTg > 1:878674/1‑70 (MQ=255)
tACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTa < 1:4004429/56‑1 (MQ=255)
acacCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAgg < 1:82684/70‑1 (MQ=255)
acacCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAgg > 1:3906360/1‑70 (MQ=255)
cacCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAttt < 1:91740/53‑1 (MQ=255)
aGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggca < 1:3252179/71‑1 (MQ=255)
aGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggca < 1:4107620/71‑1 (MQ=255)
ggAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcag > 1:4146149/1‑71 (MQ=255)
ggAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcag > 1:4141077/1‑71 (MQ=255)
ggAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcag < 1:2940368/71‑1 (MQ=255)
gAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCgg < 1:1450121/67‑1 (MQ=255)
gAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCgg < 1:3169262/67‑1 (MQ=255)
aCTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:2301031/1‑70 (MQ=255)
aCTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:613314/1‑70 (MQ=255)
tGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:1510548/1‑68 (MQ=255)
tGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:2715643/1‑68 (MQ=255)
gTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGc > 1:1330063/1‑52 (MQ=255)
ttGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGc < 1:861158/71‑1 (MQ=255)
tGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGc > 1:767856/1‑70 (MQ=255)
tGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGc > 1:1013118/1‑70 (MQ=255)
gagaAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGc > 1:2770061/1‑41 (MQ=255)
gagaAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTAttt > 1:3507845/1‑47 (MQ=255)
agaAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGGAAc < 1:2718823/71‑1 (MQ=255)
agaAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAc < 1:1099475/71‑1 (MQ=255)
gaAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcg < 1:2108340/71‑1 (MQ=255)
aaCGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcga < 1:1601151/70‑1 (MQ=255)
gcgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCt > 1:1062140/1‑36 (MQ=255)
gcgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGa > 1:2840885/1‑46 (MQ=255)
cgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccg < 1:1439531/71‑1 (MQ=255)
cgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccg < 1:1093912/71‑1 (MQ=255)
ggAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccg < 1:691705/70‑1 (MQ=255)
ggAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccga < 1:3783706/71‑1 (MQ=255)
aGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccgaag > 1:1966307/1‑71 (MQ=255)
|
CATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAACGAAGCCGAAG > W3110S.gb/4012306‑4012445
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
