Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
385,241 |
G→A |
8.6% |
V151I (GTC→ATC) |
cstA → |
carbon starvation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 385,241 | 0 | G | A | 8.6%
| 168.1
/ 5.3
| 58 | V151I (GTC→ATC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base G (23/30); new base A (3/2); total (26/32) |
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 |
CGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAT > minE/385171‑385308
|
cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTgg < 1:3503223/71‑1 (MQ=255)
gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaag > 1:3469037/1‑68 (MQ=255)
cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga < 1:3604802/71‑1 (MQ=255)
cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAaagaa > 1:2566236/1‑69 (MQ=255)
aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg > 1:1667077/1‑71 (MQ=255)
tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGTGCTGGTCAAAGAAGAGATgg > 1:2983340/1‑71 (MQ=255)
tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg < 1:3428842/71‑1 (MQ=255)
tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg < 1:2382705/71‑1 (MQ=255)
tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg < 1:150903/71‑1 (MQ=255)
tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg < 1:1753496/71‑1 (MQ=255)
taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac > 1:3188782/2‑69 (MQ=255)
taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac > 1:1700810/2‑69 (MQ=255)
taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg < 1:122186/68‑1 (MQ=255)
cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc > 1:724233/1‑71 (MQ=255)
tGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc < 1:766422/70‑1 (MQ=255)
tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg < 1:2645659/63‑1 (MQ=255)
tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg < 1:38885/62‑1 (MQ=255)
cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCTCTGGGTGAGCTGGTCAAAGAAGAGATg < 1:3191507/60‑1 (MQ=255)
cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg < 1:3880871/60‑1 (MQ=255)
cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc > 1:1328731/1‑71 (MQ=255)
tgtttgttTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga > 1:3225604/1‑55 (MQ=255)
gtttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg > 1:2727909/1‑71 (MQ=255)
tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga > 1:737161/1‑55 (MQ=255)
tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg < 1:2236217/58‑1 (MQ=255)
tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc < 1:3240845/69‑1 (MQ=255)
tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg < 1:1378872/71‑1 (MQ=255)
tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg > 1:3492533/1‑57 (MQ=255)
tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg > 1:2850384/1‑57 (MQ=255)
tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg > 1:3356221/1‑71 (MQ=255)
tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg > 1:1070097/1‑71 (MQ=255)
tttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt < 1:3695223/70‑1 (MQ=255)
tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt < 1:729648/50‑1 (MQ=255)
tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa > 1:2277568/1‑57 (MQ=255)
tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa < 1:2329850/70‑1 (MQ=255)
cTACGCGCCGTGACGGTCGC‑TCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt > 1:1979833/1‑71 (MQ=255)
cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt > 1:2331255/1‑71 (MQ=255)
cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt > 1:1375815/1‑71 (MQ=255)
cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt < 1:3189982/70‑1 (MQ=255)
aCGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg < 1:851634/48‑1 (MQ=255)
cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc < 1:548553/70‑1 (MQ=255)
cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc < 1:1671666/70‑1 (MQ=255)
ccGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtgg < 1:1977237/71‑1 (MQ=255)
gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga > 1:2444047/1‑37 (MQ=255)
gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt < 1:1411758/38‑1 (MQ=255)
aCGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGcc > 1:383128/1‑71 (MQ=255)
tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATgggg > 1:3600125/1‑37 (MQ=255)
tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt > 1:1313037/1‑71 (MQ=255)
tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt < 1:2502337/71‑1 (MQ=255)
gc‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt < 1:1056735/71‑1 (MQ=255)
gc‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt < 1:1779906/71‑1 (MQ=255)
c‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt < 1:1563325/52‑1 (MQ=255)
gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt > 1:490806/1‑48 (MQ=255)
gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGTTGGCCTGCTTTATGa > 1:2309060/1‑71 (MQ=255)
ggggAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCa < 1:1366602/67‑1 (MQ=255)
gTGAGCTGGTCAAAGAAGAGATGGGGCCAACTGCCGGGGTGATTGCGCtggtg < 1:3630862/53‑1 (MQ=255)
aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATg < 1:801091/62‑1 (MQ=255)
aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGt < 1:1056047/70‑1 (MQ=255)
aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTc < 1:588010/71‑1 (MQ=255)
cTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAt > 1:2002480/1‑71 (MQ=255)
ggTCAAAGAAGAGATGGGGCTAACCGCCGGGGTGATTgc < 1:2102934/39‑1 (MQ=255)
|
CGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAT > minE/385171‑385308
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
