Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
385,816 |
T→C |
20.3% |
H342H (CAT→CAC) |
cstA → |
carbon starvation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 385,816 | 0 | T | C | 20.3%
| 116.8
/ 25.9
| 59 | H342H (CAT→CAC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base T (27/20); new base C (3/9); total (30/29) |
| Fisher's exact test for biased strand distribution p-value = 5.75e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 |
GGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGC > minE/385747‑385882
|
ggTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCAGCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg > 1:3508245/1‑71 (MQ=255)
tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCt < 1:1213685/71‑1 (MQ=255)
ggACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCt > 1:2096827/1‑70 (MQ=255)
aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt < 1:551934/71‑1 (MQ=255)
ccGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCAt > 1:3712824/1‑62 (MQ=255)
tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt < 1:2811760/71‑1 (MQ=255)
aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:1538696/1‑71 (MQ=255)
aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:589601/1‑71 (MQ=255)
aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:1879872/1‑71 (MQ=255)
ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt < 1:3291672/59‑1 (MQ=255)
ttCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCggta < 1:516376/70‑3 (MQ=255)
tCCCGTTCATGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc > 1:2241936/1‑70 (MQ=255)
ccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga < 1:198973/70‑3 (MQ=255)
ccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga < 1:2624561/70‑3 (MQ=255)
cGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc > 1:3610705/1‑70 (MQ=255)
cGTTCCTGTTGATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTacgacg > 1:3111843/1‑71 (MQ=255)
cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAGGCCGaa < 1:513094/71‑1 (MQ=255)
gTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc > 1:2085774/1‑51 (MQ=255)
gTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:871837/1‑67 (MQ=255)
gTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga < 1:345865/62‑3 (MQ=255)
tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgca > 1:2946110/1‑41 (MQ=255)
aTCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCt < 1:2748866/71‑1 (MQ=255)
aTCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc < 1:3476404/70‑1 (MQ=255)
aTCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCt > 1:793095/1‑71 (MQ=255)
tCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTg < 1:1647770/71‑1 (MQ=255)
tCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTg < 1:652499/71‑1 (MQ=255)
aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGAt > 1:301806/1‑41 (MQ=255)
ccATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc < 1:3153357/66‑1 (MQ=255)
cATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGGTGATCTCTTCTGGGACCACGc > 1:973459/1‑56 (MQ=255)
aTCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaa < 1:983120/71‑1 (MQ=255)
aTCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaa < 1:609374/71‑1 (MQ=255)
tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac > 1:3689463/1‑71 (MQ=255)
gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga < 1:2135801/71‑1 (MQ=255)
gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:1849348/71‑1 (MQ=255)
ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaa < 1:2030088/71‑1 (MQ=255)
ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaa < 1:2278416/71‑1 (MQ=255)
cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg < 1:1613947/71‑1 (MQ=255)
cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg > 1:2297925/1‑71 (MQ=255)
tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaacc > 1:3047714/1‑67 (MQ=255)
tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaac < 1:3812158/69‑3 (MQ=255)
tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGc < 1:277114/47‑1 (MQ=255)
tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGc < 1:2970180/71‑1 (MQ=255)
gcgcGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcga < 1:910831/60‑1 (MQ=255)
gcgcGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAg < 1:3158300/71‑1 (MQ=255)
gtgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGt > 1:2231017/1‑70 (MQ=255)
tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:2903705/1‑71 (MQ=255)
tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:2899356/1‑71 (MQ=255)
tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:969233/1‑71 (MQ=255)
cTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt > 1:3010349/1‑70 (MQ=255)
tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTg < 1:1400676/66‑1 (MQ=255)
tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa > 1:611994/1‑71 (MQ=255)
gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa < 1:3108896/69‑1 (MQ=255)
gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATc > 1:2382780/1‑71 (MQ=255)
cTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTg > 1:3858316/1‑63 (MQ=255)
cTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCg > 1:805865/1‑71 (MQ=255)
ttCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcg > 1:1392380/1‑45 (MQ=255)
ttCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCgg > 1:1162794/1‑71 (MQ=255)
ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCTAACGAAGGGCAGGCGTGCTTTAt > 1:1428709/1‑66 (MQ=255)
ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAgg < 1:230622/51‑1 (MQ=255)
ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGc > 1:819680/1‑70 (MQ=255)
|
GGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGC > minE/385747‑385882
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
