Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 917,592 A→G 31.2% L311L (TTA→TTG)  tyrR → DNA‑binding transcriptional dual regulator, tyrosine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE917,5920AG31.2% 118.9 / 78.7 96L311L (TTA→TTG) tyrRDNA‑binding transcriptional dual regulator, tyrosine‑binding
Reads supporting (aligned to +/- strand):  ref base A (35/31);  new base G (17/13);  total (52/44)
Fisher's exact test for biased strand distribution p-value = 8.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 101 total aligned reads displayed.

AGCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGG  >  minE/917522‑917662
                                                                      |                                                                      
aGCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTa                                                                        <  1:2542468/71‑1 (MQ=255)
aGCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTa                                                                        <  1:1599195/71‑1 (MQ=255)
 gCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTa                                                                        <  1:2538038/70‑1 (MQ=255)
   aGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTg                                                                     <  1:2286174/71‑1 (MQ=255)
    ggCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGc                                                                    >  1:3298020/1‑71 (MQ=255)
       gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt                                                                 >  1:248304/1‑71 (MQ=255)
       gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt                                                                 >  1:2631603/1‑71 (MQ=255)
       gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt                                                                 >  1:3527619/1‑71 (MQ=255)
       gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt                                                                 >  1:1445880/1‑71 (MQ=255)
       gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGtt                                                                 >  1:1854187/1‑71 (MQ=255)
       gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGtt                                                                 >  1:1433906/1‑71 (MQ=255)
        aaCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGt                                                                  <  1:3823505/69‑1 (MQ=255)
        aaCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGt                                                                  <  1:151303/69‑1 (MQ=255)
        aaCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCAAGGATGCAGGCGAAATTGCTGCGttt                                                                <  1:707026/71‑1 (MQ=255)
         aCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTc                                                               <  1:174056/71‑1 (MQ=255)
         aCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTc                                                               >  1:193614/1‑71 (MQ=255)
             tggtTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACt                                                                      >  1:2069729/1‑60 (MQ=255)
             tggtTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCtt                                                            >  1:2088330/1‑70 (MQ=255)
               gtTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAat                                                         >  1:549362/1‑71 (MQ=255)
                  cGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAATTTGCTGCGTTTCCTTAatga                                                       <  1:3675231/70‑1 (MQ=255)
                  cGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgat                                                      >  1:1811434/1‑71 (MQ=255)
                  cGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgat                                                      >  1:2789217/1‑71 (MQ=255)
                  cGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatga                                                       <  1:681513/70‑1 (MQ=255)
                   ggTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgatg                                                     >  1:1805819/1‑71 (MQ=255)
                    gTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatg                                                        <  1:1477941/67‑1 (MQ=255)
                    gTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatg                                                        <  1:2695097/67‑1 (MQ=255)
                      gCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTg                                                                     >  1:1322089/1‑52 (MQ=255)
                          ttGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCa                                                  <  1:1479268/67‑1 (MQ=255)
                          ttGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACt                                                <  1:1813710/69‑1 (MQ=255)
                           tGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACtt                                               >  1:3337659/1‑69 (MQ=255)
                           tGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTc                                             >  1:2365107/1‑71 (MQ=255)
                           tGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTaa                                                          <  1:1716863/58‑1 (MQ=255)
                            ggATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgat                                                      <  1:2151198/61‑1 (MQ=255)
                             gATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTcc                                                              <  1:3943321/52‑1 (MQ=255)
                             gATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTcc                                            <  1:3708866/70‑1 (MQ=255)
                               tGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTc                                         >  1:2283878/1‑71 (MQ=255)
                                gAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTa                                                                        <  1:1911949/39‑1 (MQ=255)
                                gAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTc                                         <  1:3192619/70‑1 (MQ=255)
                                   aTAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGt                                     >  1:3688271/1‑71 (MQ=255)
                                   aTAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGtt                                                                 <  1:3099269/43‑1 (MQ=255)
                                    tAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGtt                                    <  1:2387309/71‑1 (MQ=255)
                                    tAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGtt                                    >  1:3437998/1‑71 (MQ=255)
                                     aGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTg                                   >  1:2159475/1‑71 (MQ=255)
                                     aGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTg                                   >  1:3526567/1‑71 (MQ=255)
                                     aGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTg                                   >  1:3442437/1‑71 (MQ=255)
                                      ggggAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTgg                                  <  1:2427562/71‑1 (MQ=255)
                                        ggAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg                                <  1:1155718/71‑1 (MQ=255)
                                        ggAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg                                <  1:3853865/71‑1 (MQ=255)
                                         gAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg                                >  1:144984/1‑70 (MQ=255)
                                          aaaTGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGaa                              <  1:2429075/71‑1 (MQ=255)
                                          aaaTGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg                                >  1:56664/1‑69 (MQ=255)
                                              gTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgatg                                                     >  1:126089/1‑44 (MQ=255)
                                              gTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGAc                           >  1:2521764/1‑70 (MQ=255)
                                               tCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatga                                                       <  1:1864749/41‑1 (MQ=255)
                                               tCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCa                         <  1:2042627/71‑1 (MQ=255)
                                               tCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCa                         >  1:3557216/1‑71 (MQ=255)
                                                caccacGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt                        >  1:128124/1‑71 (MQ=255)
                                                 accacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt                        >  1:2795674/1‑70 (MQ=255)
                                                  ccacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt                        >  1:572082/1‑69 (MQ=255)
                                                  ccacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt                        >  1:3261174/1‑69 (MQ=255)
                                                   cacGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCa                                                  <  1:485681/42‑1 (MQ=255)
                                                   cacGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCa                                                  <  1:3436997/42‑1 (MQ=255)
                                                   cacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGaa                              >  1:3373629/1‑62 (MQ=255)
                                                   cacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt                        <  1:2830413/68‑1 (MQ=255)
                                                    acGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACt                                                >  1:430621/1‑43 (MQ=255)
                                                    acGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACt                                                >  1:2144166/1‑43 (MQ=255)
                                                     cGGATGCAGGCGAAATTGCTGCGGTTCCTTAATGATGGCa                                                  <  1:820381/40‑1 (MQ=37)
                                                     cGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGt                   <  1:2386133/71‑1 (MQ=255)
                                                     cGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGt                   <  1:1826681/71‑1 (MQ=255)
                                                        aTGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCa                                                  <  1:3441406/37‑1 (MQ=255)
                                                        aTGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGAcc                          >  1:2357054/1‑61 (MQ=255)
                                                         tGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCAt               <  1:2907132/71‑1 (MQ=255)
                                                            aGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCAt               >  1:648603/1‑68 (MQ=255)
                                                            aGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCAt               >  1:437773/1‑68 (MQ=255)
                                                            aGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt                        <  1:3508739/59‑1 (MQ=255)
                                                            aGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt                        <  1:3682791/59‑1 (MQ=255)
                                                             ggCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTc            >  1:2815529/1‑70 (MQ=255)
                                                             ggCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCg           <  1:559503/71‑1 (MQ=255)
                                                              gCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGt                                          <  1:2516872/39‑1 (MQ=255)
                                                              gCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGt                                          <  1:151384/39‑1 (MQ=255)
                                                               cGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAt         <  1:2211352/71‑1 (MQ=255)
                                                                gAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCggg                                      >  1:123837/1‑41 (MQ=255)
                                                                gAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtg        >  1:1367852/1‑71 (MQ=255)
                                                                gAAATTGCTGCGTTTCCTTAATGATGGCACTTTCAGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtg        >  1:2381399/1‑71 (MQ=255)
                                                                gAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtg        <  1:3523451/71‑1 (MQ=255)
                                                                  aaTTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtgtg      >  1:3886573/1‑71 (MQ=255)
                                                                  aaTTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtgtg      >  1:1854686/1‑71 (MQ=255)
                                                                  aaTTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtg        >  1:3552680/1‑69 (MQ=255)
                                                                  aaTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtgtg      >  1:3526087/1‑71 (MQ=255)
                                                                   aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTc            <  1:2801300/64‑1 (MQ=255)
                                                                   aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTc            <  1:2374632/64‑1 (MQ=255)
                                                                   aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGc     >  1:2964896/1‑71 (MQ=255)
                                                                   aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGc     >  1:3852266/1‑71 (MQ=255)
                                                                   aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGc     <  1:169504/71‑1 (MQ=255)
                                                                    ttACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg                                <  1:2420187/43‑1 (MQ=255)
                                                                     tACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGa                      >  1:3196513/1‑52 (MQ=255)
                                                                     tACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCg           >  1:3790808/1‑63 (MQ=255)
                                                                     tACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCgg   >  1:1276041/1‑71 (MQ=255)
                                                                     tACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCgg   >  1:1132932/1‑71 (MQ=255)
                                                                      aCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCggg  >  1:140104/1‑71 (MQ=255)
                                                                      |                                                                      
AGCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGG  >  minE/917522‑917662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A