Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,026,414 |
G→A |
28.2% |
intergenic (‑173/+61) |
plsC ← / ← parC |
1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase/DNA topoisomerase IV, subunit A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,026,414 | 0 | G | A | 28.2%
| 68.3
/ 29.6
| 46 | intergenic (‑173/+61) | plsC/parC | 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase/DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base G (10/23); new base A (6/7); total (16/30) |
| Fisher's exact test for biased strand distribution p-value = 3.28e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 |
CATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATAT > minE/2026347‑2026475
|
cATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACAACt < 1:2410535/71‑1 (MQ=255)
tGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTa < 1:2697975/71‑1 (MQ=255)
aaTAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGTGTTGAATACGCTGCCGGATGACAACTTaa < 1:1071133/70‑1 (MQ=255)
aTAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACg < 1:2752891/71‑1 (MQ=255)
aTAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACg > 1:3457167/1‑71 (MQ=255)
acaacGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTc < 1:1048795/71‑1 (MQ=255)
caacGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCa < 1:2172273/71‑1 (MQ=255)
ggTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCg < 1:55748/71‑1 (MQ=255)
aaGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACAACTTAACGTTTCATCCGGCGTTc > 1:3376405/1‑71 (MQ=255)
aaGGGAATTTTTATCGTTTCC‑‑GGGTTGAATACGCTGCCGGATGACAACTTAACGTTTCATCCGGCGTTcc < 1:712801/70‑1 (MQ=255)
aGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTcc < 1:2173621/71‑1 (MQ=255)
gAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTg > 1:1633426/1‑71 (MQ=255)
aaTTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTg > 1:1241884/1‑70 (MQ=255)
aTTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGt > 1:1848779/1‑51 (MQ=255)
aTTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGt > 1:1118640/1‑51 (MQ=255)
tttttATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACAACTTAACGTTTCATCCGGCGTTCCTTGc > 1:738873/1‑69 (MQ=255)
tttATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAg < 1:3878152/70‑1 (MQ=255)
tttATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAg < 1:333794/70‑1 (MQ=255)
tCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCggg < 1:571258/70‑1 (MQ=255)
tCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCggg < 1:3331750/70‑1 (MQ=255)
gTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGt > 1:2424388/1‑54 (MQ=255)
gTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGggag < 1:1695605/70‑1 (MQ=255)
gTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGggag < 1:3401091/70‑1 (MQ=255)
tttCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGggag < 1:1999039/69‑1 (MQ=255)
tttCCGGGGGTTGAATACGCTGCCGGATGACAACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGggagga < 1:2390541/71‑1 (MQ=255)
tCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGaaa > 1:3035065/1‑71 (MQ=255)
cGGGGGTTGAATACGCTGCCGGATGACAACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGaaa < 1:196524/69‑1 (MQ=255)
gggggTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAg < 1:1680763/71‑1 (MQ=255)
ggggTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAg > 1:2659607/1‑70 (MQ=255)
ggggTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAg < 1:2390752/70‑1 (MQ=255)
ttGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGccc < 1:139113/71‑1 (MQ=255)
aTACGCTGCCGGATGACAACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTccc > 1:834232/1‑71 (MQ=255)
tACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGggagga < 1:356957/56‑1 (MQ=255)
tACGCTGCCGGATGACAACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTcccc > 1:1109005/1‑71 (MQ=255)
aCGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCg > 1:2887142/1‑71 (MQ=255)
cGCTGCCGGATGACAACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCg < 1:997647/70‑1 (MQ=255)
tGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGggagga < 1:3138873/51‑1 (MQ=255)
gCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCata > 1:456757/1‑71 (MQ=255)
gCCGGATGACAACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCata > 1:827867/1‑71 (MQ=255)
ccGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGgga < 1:3185301/46‑1 (MQ=255)
ccGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCatat < 1:362092/71‑1 (MQ=255)
ccGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCatat < 1:748149/71‑1 (MQ=255)
ccGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCatat < 1:752761/71‑1 (MQ=255)
ccGGATGACAACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCatat > 1:3854940/1‑71 (MQ=255)
cGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCgg < 1:2628094/65‑1 (MQ=255)
tGACAACTTAACGTTTCATCCGGCGTTCCTTGCAAg < 1:3617025/36‑1 (MQ=255)
|
CATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATAT > minE/2026347‑2026475
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
