Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,292,617 |
A→G |
51.7% |
intergenic (+168/‑253) |
fsaB → / → gldA |
fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,292,617 | 0 | A | G | 51.7%
| 4.6
/ 76.0
| 58 | intergenic (+168/‑253) | fsaB/gldA | fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
| Reads supporting (aligned to +/- strand): ref base A (13/15); new base G (14/16); total (27/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TATTAGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGC > minE/2292549‑2292687
|
tattaGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGAc > 1:1806757/1‑71 (MQ=255)
tattaGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGAc > 1:1717600/1‑71 (MQ=255)
ttaGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACgg < 1:2191904/71‑1 (MQ=255)
taGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATACCCAGGGCGGAAACgg > 1:3649662/1‑70 (MQ=255)
gCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCAt > 1:2736979/1‑71 (MQ=255)
gCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCAt > 1:1264819/1‑71 (MQ=255)
gCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCAt > 1:3872384/1‑71 (MQ=255)
cGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTc > 1:1099856/1‑71 (MQ=255)
cGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTc > 1:2359933/1‑71 (MQ=255)
gCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCg < 1:2436150/71‑1 (MQ=255)
gCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCg > 1:1162525/1‑71 (MQ=255)
cTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTAc > 1:1889125/1‑71 (MQ=255)
aGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGtt > 1:1522448/1‑70 (MQ=255)
cAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAg < 1:2490910/71‑1 (MQ=255)
cAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAg > 1:2686493/1‑71 (MQ=255)
aaaTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGc < 1:3519674/70‑1 (MQ=255)
cGTTGCTCCGTATGTTAACCGCGTAGATGACCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCaaa > 1:804589/1‑71 (MQ=255)
gTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCaaa > 1:1622517/1‑70 (MQ=255)
gCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCt > 1:3718908/1‑71 (MQ=255)
cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCAtt < 1:3338192/44‑1 (MQ=255)
cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCAtt < 1:2547203/44‑1 (MQ=255)
cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCAtt < 1:3877418/44‑1 (MQ=255)
cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCt < 1:1339555/70‑1 (MQ=255)
cTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCa < 1:416351/64‑1 (MQ=255)
tCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAgg < 1:131046/56‑1 (MQ=255)
tCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAg < 1:1059568/58‑1 (MQ=255)
cGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGt > 1:681528/1‑69 (MQ=255)
cGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGc > 1:3431525/1‑60 (MQ=255)
cGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGt < 1:3690559/69‑1 (MQ=255)
gTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGtt < 1:1984820/69‑1 (MQ=255)
aTGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTc < 1:1725823/48‑1 (MQ=255)
aTGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa > 1:3084988/1‑70 (MQ=255)
aTGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa > 1:922830/1‑70 (MQ=255)
tGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:94970/70‑1 (MQ=255)
tGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:3300024/70‑1 (MQ=255)
tGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:1368391/70‑1 (MQ=255)
ttAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAAt < 1:3882769/70‑1 (MQ=255)
tAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCg > 1:1487228/1‑36 (MQ=255)
aaCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGc < 1:1163760/70‑1 (MQ=255)
aCCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCAc < 1:2731668/71‑1 (MQ=255)
aCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCAc > 1:1969961/1‑71 (MQ=255)
aCCGCCTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACCCTGTTAGAAATGCAc < 1:501305/71‑1 (MQ=255)
cgTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGTAGCTGCAAACGCTGTTAGAAATGCACgcgc < 1:178876/71‑1 (MQ=255)
gTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCAcgcg > 1:2832897/1‑69 (MQ=255)
tAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCt > 1:2700980/1‑43 (MQ=255)
tAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCa < 1:962730/37‑1 (MQ=255)
tAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCa < 1:3085428/37‑1 (MQ=255)
tGCCCAGGGCGGAAACGTCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGaaa < 1:499470/71‑1 (MQ=255)
gCCCAGGGCGGAGACGGCATTCGTATGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg > 1:383819/1‑71 (MQ=255)
cccAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg < 1:2537589/70‑1 (MQ=255)
cccAGGGCGGAGACGACATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg < 1:1091303/70‑1 (MQ=255)
ccAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCa > 1:2009602/1‑71 (MQ=255)
aGGGCGGAGACGACATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATg > 1:1373837/1‑71 (MQ=255)
aGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATg > 1:3626611/1‑71 (MQ=255)
ggCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGt < 1:705746/71‑1 (MQ=255)
ggCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGt < 1:3157331/71‑1 (MQ=255)
gCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTg < 1:3375811/71‑1 (MQ=255)
cGGAAAGGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATTGTGc < 1:1687632/71‑1 (MQ=255)
aaaCGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTgg > 1:2364351/1‑71 (MQ=255)
aaCGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGc < 1:2197832/71‑1 (MQ=255)
|
TATTAGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGC > minE/2292549‑2292687
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
