Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,595,611 |
G→T |
17.0% |
A329A (GCC→GCA) |
arsB ← |
arsenite/antimonite transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,595,611 | 0 | G | T | 17.0%
| 127.8
/ 10.2
| 47 | A329A (GCC→GCA) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (12/27); new base T (2/6); total (14/33) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 |
CCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAG > minE/2595549‑2595681
|
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:2708672/69‑1 (MQ=255)
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:1184608/69‑1 (MQ=255)
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:1307564/69‑1 (MQ=255)
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:1413277/69‑1 (MQ=255)
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa < 1:1579238/70‑1 (MQ=255)
cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg > 1:3614976/1‑70 (MQ=255)
aTCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg > 1:2517067/1‑70 (MQ=255)
tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa < 1:2784911/71‑1 (MQ=255)
tCAATCGACAGCGAACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGc > 1:3547802/1‑62 (MQ=255)
cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa > 1:454727/1‑66 (MQ=255)
cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt > 1:1365583/1‑71 (MQ=255)
aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:2062411/1‑71 (MQ=255)
aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaag < 1:1858041/70‑2 (MQ=255)
aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGACGAGAGAAATGCCGTCAAGaagc < 1:1880062/71‑3 (MQ=255)
aTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATcc > 1:2230069/1‑71 (MQ=255)
tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg < 1:3703989/71‑1 (MQ=255)
tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg > 1:3476805/1‑71 (MQ=255)
gACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt < 1:1177483/71‑1 (MQ=255)
gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGTCGGTGAGGAATCCGGt < 1:2611145/71‑1 (MQ=255)
gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg < 1:817798/70‑1 (MQ=255)
gcgcaCCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:2432020/70‑1 (MQ=255)
aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg > 1:773494/1‑51 (MQ=255)
aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg > 1:2587318/1‑51 (MQ=255)
aCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgc < 1:317021/71‑1 (MQ=255)
tcAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcgat > 1:1449819/2‑69 (MQ=255)
ccAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgcg > 1:185886/1‑71 (MQ=255)
agcaCCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGc < 1:3215003/68‑1 (MQ=255)
aCCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCca < 1:2235688/71‑1 (MQ=255)
cGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCcac < 1:1424282/70‑1 (MQ=255)
gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg < 1:3928906/71‑1 (MQ=255)
tCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGa < 1:249413/54‑1 (MQ=255)
tCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCcaca < 1:1126770/69‑1 (MQ=255)
ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:536063/1‑43 (MQ=255)
ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc < 1:2856962/71‑1 (MQ=255)
gCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGcc < 1:3830264/49‑1 (MQ=255)
atatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgcg < 1:3333826/57‑1 (MQ=255)
tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt < 1:3353065/43‑1 (MQ=255)
gTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATcc < 1:1177307/70‑1 (MQ=255)
ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccccc > 1:810155/1‑49 (MQ=255)
taGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg < 1:3353876/71‑1 (MQ=255)
taGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg < 1:1827776/71‑1 (MQ=255)
taGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg < 1:3340122/71‑1 (MQ=255)
gaagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg < 1:1009229/69‑1 (MQ=255)
agagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTg < 1:1623860/70‑1 (MQ=255)
agagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGa < 1:1726669/71‑1 (MQ=255)
gAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGcc < 1:822839/55‑1 (MQ=255)
gAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGc < 1:2603276/71‑1 (MQ=255)
ggCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGc > 1:3044490/1‑68 (MQ=255)
ggCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAg < 1:852350/71‑1 (MQ=255)
|
CCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAG > minE/2595549‑2595681
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
