Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,892,348 |
G→A |
33.3% |
intergenic (+46/‑94) |
ytfQ → / → ytfR |
predicted sugar transporter subunit/predicted sugar transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,892,348 | 0 | G | A | 33.3%
| 69.0
/ 48.7
| 60 | intergenic (+46/‑94) | ytfQ/ytfR | predicted sugar transporter subunit/predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (21/19); new base A (11/9); total (32/28) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTG > minE/2892279‑2892414
|
aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTcg < 1:297379/70‑1 (MQ=255)
tgcctgatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCAcgcc > 1:212568‑M2/43‑71 (MQ=255)
tgcctgatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCAcgcc > 1:3638845‑M2/43‑71 (MQ=255)
gcctgatgcgctacgcttatcaggcctacagattcttgagccATTCGTAGGCCGGATAAGGCGTTCacgc > 1:434558‑M2/43‑70 (MQ=255)
agaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCgcgc > 1:2152367/1‑69 (MQ=255)
ctgatgcgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCAcgccgc < 1:2768907‑M2/31‑1 (MQ=255)
cctgatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCAcgcc < 1:1742409‑M2/29‑1 (MQ=255)
gatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:3424724‑M2/39‑71 (MQ=255)
atgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1417802‑M2/38‑71 (MQ=255)
atgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1698281‑M2/38‑71 (MQ=255)
atgcgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATc < 1:3367228‑M2/34‑1 (MQ=255)
tgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:1593977‑M2/36‑71 (MQ=255)
cgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg < 1:2959088‑M2/36‑1 (MQ=255)
cgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCgg < 1:2360872‑M2/37‑1 (MQ=255)
tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGc < 1:104092/70‑1 (MQ=255)
ctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCGgca < 1:1879157‑M2/39‑2 (MQ=255)
tacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCggcat > 1:2696707‑M2/32‑69 (MQ=255)
tacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCggcag > 1:1085164‑M2/32‑69 (MQ=255)
tacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt > 1:1709259‑M2/31‑71 (MQ=255)
gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa < 1:47971/70‑1 (MQ=255)
ggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa < 1:712316/69‑1 (MQ=255)
cgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCggcag < 1:2136661‑M2/40‑3 (MQ=255)
ttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCa > 1:3110272‑M2/27‑58 (MQ=255)
ttGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTg > 1:2705674/1‑70 (MQ=255)
tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgccgc > 1:3850029/1‑55 (MQ=255)
tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa > 1:1298471/1‑64 (MQ=255)
atcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTg < 1:339089‑M2/45‑1 (MQ=255)
atcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTg < 1:585171‑M2/45‑1 (MQ=255)
gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCg > 1:3652576/1‑59 (MQ=18)
gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATcc < 1:1574127/58‑1 (MQ=255)
gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGt > 1:995263/1‑67 (MQ=255)
gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCa < 1:3526392/70‑1 (MQ=255)
gAGGGGTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTcgcg < 1:1045342/50‑1 (MQ=255)
aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACt < 1:1563226/71‑1 (MQ=255)
gTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGaa < 1:3731858/71‑1 (MQ=255)
ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgcc > 1:2165178/1‑47 (MQ=255)
ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgcc > 1:3394226/1‑47 (MQ=255)
ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATg < 1:933582/60‑1 (MQ=255)
acagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCggcaa > 1:41356‑M2/16‑53 (MQ=255)
tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAGGcat > 1:1511977/1‑68 (MQ=255)
aTGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGa > 1:3206027/1‑71 (MQ=255)
tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg < 1:3604240/69‑1 (MQ=255)
gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTg < 1:3466736/56‑1 (MQ=255)
aTGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACg > 1:3843917/1‑71 (MQ=255)
gATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc > 1:365581/1‑71 (MQ=255)
aTTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc > 1:72494/1‑71 (MQ=255)
ttCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcg > 1:3310600/1‑71 (MQ=255)
cGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc < 1:3038261/70‑1 (MQ=255)
gTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgcg < 1:1273212/70‑1 (MQ=255)
ggCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt > 1:3180584/1‑70 (MQ=255)
ggCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt < 1:2253388/70‑1 (MQ=255)
gCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa < 1:275933/71‑1 (MQ=255)
gCCGGATAAGGCGCACGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa < 1:2195048/71‑1 (MQ=255)
cGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTAt > 1:501586/1‑70 (MQ=255)
ggATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa < 1:124883/71‑1 (MQ=255)
aTAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg < 1:2173154/71‑1 (MQ=255)
aTAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg > 1:1935292/1‑71 (MQ=255)
tAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc > 1:2525184/1‑71 (MQ=255)
tAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc > 1:723037/1‑71 (MQ=255)
gcgcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa < 1:430234/70‑1 (MQ=255)
gcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCtt < 1:450083/58‑1 (MQ=255)
gcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa > 1:1043045/1‑68 (MQ=255)
gcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg > 1:1047539/1‑71 (MQ=255)
gcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg > 1:1121590/1‑71 (MQ=255)
|
AAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTG > minE/2892279‑2892414
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
