Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,462 G→C 14.5% G78G (GGC→GGG)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,4620GC14.5% 238.2 / 27.5 96G78G (GGC→GGG) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base G (47/35);  new base C (8/6);  total (55/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAG  >  minE/2919394‑2919531
                                                                    |                                                                     
tACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGcc                                                                     <  1:3711769/71‑1 (MQ=255)
  cATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGcccta                                                                   <  1:1101366/71‑3 (MQ=255)
   aTGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGcc                                                                     >  1:2753508/1‑68 (MQ=255)
   aTGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGcc                                                                     >  1:3325441/1‑68 (MQ=255)
     gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgaga                                                                 >  1:1671216/1‑70 (MQ=255)
     gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgaga                                                                 >  1:286668/1‑70 (MQ=255)
     gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgaga                                                                 >  1:2474233/1‑70 (MQ=255)
      cGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGAt                                                                >  1:628717/1‑70 (MQ=255)
       gACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATaa                                                              <  1:1545066/71‑1 (MQ=255)
       gACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATaa                                                              <  1:3385590/71‑1 (MQ=255)
         ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg                                                             >  1:3253438/1‑70 (MQ=255)
         ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg                                                             >  1:1435759/1‑70 (MQ=255)
         ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg                                                             >  1:3744153/1‑70 (MQ=255)
         ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg                                                             >  1:2606645/1‑70 (MQ=255)
          cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTa                                                           <  1:1338284/71‑1 (MQ=255)
          cAAGCACGCGGGCAGTGTCTTTAATAGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                            >  1:3808790/1‑70 (MQ=255)
            aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAc                                                         >  1:2019871/1‑71 (MQ=255)
            aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAc                                                         <  1:800302/71‑1 (MQ=255)
              cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                       <  1:198903/71‑1 (MQ=255)
              cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcg                                                        >  1:1024561/1‑70 (MQ=255)
                cgcgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                     <  1:1212140/71‑1 (MQ=255)
                 gcgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAg                                                    >  1:3674753/1‑71 (MQ=255)
                 gcgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAg                                                    >  1:2676850/1‑71 (MQ=255)
                      cAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc                                               >  1:1171111/1‑71 (MQ=255)
                        gtgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGccc                                               <  1:1796656/69‑1 (MQ=255)
                         tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                          >  1:2262576/1‑57 (MQ=255)
                         tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTgg                                            >  1:305225/1‑71 (MQ=255)
                         tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTgg                                            >  1:3772954/1‑71 (MQ=255)
                         tgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAAc                                                         >  1:1536508/1‑58 (MQ=255)
                          gtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTgg                                            <  1:1451349/70‑1 (MQ=255)
                          gtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGt                                           >  1:2564401/1‑71 (MQ=255)
                          gtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAAcgc                                                       <  1:1223077/59‑1 (MQ=255)
                           tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCga                                                                   >  1:1111400/1‑46 (MQ=255)
                           tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                     >  1:3227485/1‑60 (MQ=255)
                           tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                     >  1:140120/1‑60 (MQ=255)
                           tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                     >  1:135462/1‑60 (MQ=255)
                             tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                            <  1:3029960/51‑1 (MQ=255)
                             tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                       <  1:2679631/56‑1 (MQ=255)
                             tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAg                                                    >  1:456162/1‑59 (MQ=255)
                             tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                         >  1:3480390/1‑70 (MQ=255)
                             tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                         >  1:910340/1‑70 (MQ=255)
                               tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat                                      >  1:3334071/1‑71 (MQ=255)
                               tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCatat                                      >  1:1032572/1‑71 (MQ=255)
                               tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAAGCGAGCACCCTGGTCatat                                      >  1:3624586/1‑71 (MQ=255)
                                aaTTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCt                                              >  1:2810014/1‑62 (MQ=255)
                                 aTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAc                                                         <  1:3024510/50‑1 (MQ=255)
                                  tCGACTCTTTATGACCAATCTGGCTGACGCTTGGGCCGAGATAAGTAACGCGAGCACCCt                                              >  1:1648351/1‑60 (MQ=255)
                                    gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgag                                                                  <  1:1462822/38‑1 (MQ=255)
                                     aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGAt                                                                <  1:889712/39‑1 (MQ=255)
                                     aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                          <  1:1961293/45‑1 (MQ=255)
                                     aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCa                                <  1:1679702/71‑1 (MQ=255)
                                      ctctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCaa                               >  1:3308221/1‑71 (MQ=255)
                                       tctTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGc                                 >  1:1662501/1‑68 (MQ=255)
                                       tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCaa                               >  1:2704415/1‑70 (MQ=255)
                                       tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAAc                              >  1:788008/1‑71 (MQ=255)
                                         tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAAc                              <  1:962910/69‑1 (MQ=255)
                                         tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCATGGTCATATGCGGCAAc                              <  1:1954740/69‑1 (MQ=255)
                                          ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc                                 <  1:2668362/65‑1 (MQ=255)
                                           tATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTc                           <  1:339524/70‑1 (MQ=255)
                                           tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                           >  1:575732/1‑70 (MQ=255)
                                            aTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                          >  1:2501674/1‑38 (MQ=255)
                                            aTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg                          <  1:417330/70‑1 (MQ=255)
                                            aTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg                          >  1:3808433/1‑70 (MQ=255)
                                             tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaa                        <  1:1746299/71‑1 (MQ=255)
                                             tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                         <  1:773765/70‑1 (MQ=255)
                                             tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                         <  1:3506041/70‑1 (MQ=255)
                                              gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGc                                    <  1:503415/58‑1 (MQ=255)
                                              gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGAACCCTTGACATATGc                                    <  1:1341441/58‑1 (MQ=255)
                                               cccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa                       <  1:3064838/69‑1 (MQ=255)
                                               cccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAag                      <  1:3312819/70‑1 (MQ=255)
                                               aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                       <  1:1027469/38‑1 (MQ=255)
                                               aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                     >  1:965550/1‑40 (MQ=255)
                                               aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa                       <  1:244577/70‑1 (MQ=255)
                                               aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAag                      <  1:2414506/71‑1 (MQ=255)
                                               aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAag                      <  1:1603931/71‑1 (MQ=255)
                                                ccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa                       >  1:1550738/1‑69 (MQ=255)
                                                ccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa                       >  1:3400372/1‑69 (MQ=255)
                                                 cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                       >  1:657330/1‑36 (MQ=255)
                                                 cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCCCCCTGGTCATATGCGGCAACTTCGAAagag                    >  1:2090553/1‑71 (MQ=255)
                                                 cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAagag                    >  1:2074159/1‑71 (MQ=255)
                                                   aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa                  <  1:3100397/71‑1 (MQ=255)
                                                    tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCAt                 >  1:1266996/1‑71 (MQ=255)
                                                     cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCa                                                  >  1:2226734/1‑37 (MQ=255)
                                                      tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGc                                    >  1:2709100/1‑50 (MQ=255)
                                                      tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCg               >  1:1136939/1‑71 (MQ=255)
                                                         cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGt            <  1:1150435/71‑1 (MQ=255)
                                                            ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc          >  1:1021955/1‑70 (MQ=255)
                                                            ccGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc          >  1:1352634/1‑70 (MQ=255)
                                                             cGCTTGGGCCGAGATAAGTAACGCGAGCACGCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc          <  1:1755589/69‑1 (MQ=255)
                                                             cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga        <  1:47219/71‑1 (MQ=255)
                                                             cGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAaga                     >  1:1256399/1‑58 (MQ=255)
                                                              gCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgag       <  1:3292804/71‑1 (MQ=255)
                                                               cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATg                                     <  1:2390389/40‑1 (MQ=255)
                                                               cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                <  1:3930069/61‑1 (MQ=255)
                                                               cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt      >  1:175560/1‑71 (MQ=255)
                                                                 tGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc     >  1:764412/1‑70 (MQ=255)
                                                                  gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcg    <  1:2916072/70‑1 (MQ=255)
                                                                  gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc     <  1:733628/69‑1 (MQ=255)
                                                                  gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc     <  1:3227791/69‑1 (MQ=255)
                                                                   ggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcgag  <  1:509753/71‑1 (MQ=255)
                                                                    |                                                                     
TACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAG  >  minE/2919394‑2919531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A