Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
285,498 |
G→A |
26.3% |
A294T (GCC→ACC) |
yagG → |
predicted sugar transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 285,498 | 0 | G | A | 26.3%
| 102.1
/ 36.2
| 57 | A294T (GCC→ACC) | yagG | predicted sugar transporter |
| Reads supporting (aligned to +/- strand): ref base G (18/24); new base A (7/8); total (25/32) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACA > W3110S.gb/285435‑285567
|
cTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTc > 1:833091/1‑69 (MQ=255)
cTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTc > 1:2680246/1‑69 (MQ=255)
cTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTc > 1:178871/1‑69 (MQ=255)
cTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCaa < 1:4335454/71‑1 (MQ=255)
tCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCaca > 1:993304/1‑69 (MQ=255)
gCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTg > 1:4460845/1‑71 (MQ=255)
ccaTGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatc < 1:2003680/71‑1 (MQ=255)
aTGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatcat > 1:1138644/1‑71 (MQ=255)
gTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCa < 1:3467335/59‑1 (MQ=255)
gTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCa < 1:773251/59‑1 (MQ=255)
gTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCa < 1:1537194/59‑1 (MQ=255)
ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatc < 1:105141/66‑1 (MQ=255)
cGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatcat > 1:4224329/1‑66 (MQ=255)
cGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCAtcgtcg > 1:1756161/1‑71 (MQ=255)
cTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGcc < 1:4341407/70‑1 (MQ=255)
cTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGcc < 1:2255818/70‑1 (MQ=255)
cTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGcc < 1:4376812/70‑1 (MQ=255)
tCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTa > 1:824693/1‑71 (MQ=255)
tCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTa > 1:2392783/1‑71 (MQ=255)
tCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa > 1:2162760/1‑71 (MQ=255)
tCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa > 1:2868838/1‑71 (MQ=255)
cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTAc > 1:4666386/1‑71 (MQ=255)
ctcctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGa > 1:1939421/1‑71 (MQ=255)
ctcctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGa > 1:2581657/1‑71 (MQ=255)
ctcctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGa > 1:1545231/1‑71 (MQ=255)
ctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatc < 1:3036208/49‑1 (MQ=255)
ctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatc < 1:167532/49‑1 (MQ=255)
tcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCt < 1:271852/65‑1 (MQ=255)
tcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCt < 1:4585183/65‑1 (MQ=255)
tcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCt < 1:3489195/65‑1 (MQ=255)
aCGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGcc > 1:2141188/1‑71 (MQ=255)
ccTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCctg > 1:2074376/1‑70 (MQ=255)
ctgctgGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCctgct < 1:1855325/71‑1 (MQ=255)
tgctgGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCAtcgtcg < 1:2393604/48‑1 (MQ=255)
ctgGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCctgctg < 1:86136/69‑1 (MQ=255)
cGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGt < 1:2507144/71‑1 (MQ=255)
cGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGt < 1:1328483/71‑1 (MQ=255)
gCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:130353/71‑1 (MQ=255)
ttCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCAc > 1:1831408/1‑71 (MQ=255)
cGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCAccc > 1:3890269/1‑71 (MQ=255)
cGACCGCGTCACCGCCTTCAAGTGGATCATCATCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCAccc > 1:3500931/1‑71 (MQ=255)
cGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCAc < 1:3751216/69‑1 (MQ=255)
cgTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:4359065/71‑1 (MQ=255)
cgTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:1309031/71‑1 (MQ=255)
cgTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:1395375/71‑1 (MQ=255)
cgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGt > 1:4892621/1‑60 (MQ=255)
cgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:3034220/71‑1 (MQ=255)
cgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:2549957/71‑1 (MQ=255)
cgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:3547618/71‑1 (MQ=255)
cgTCACCACATTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:906323/71‑1 (MQ=255)
tCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:4703878/59‑1 (MQ=255)
tCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:3109041/59‑1 (MQ=255)
tCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:490771/59‑1 (MQ=255)
tCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:2254993/59‑1 (MQ=255)
accaccTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTc > 1:3366745/1‑54 (MQ=255)
aCCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGca > 1:4857480/1‑71 (MQ=255)
aCCGCCTTAAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGca > 1:1725340/1‑71 (MQ=255)
cGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGcaca > 1:3139943/1‑71 (MQ=255)
|
CTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACA > W3110S.gb/285435‑285567
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
