Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
530,077 |
T→C |
15.4% |
Y125C (TAT→TGT) |
ybbB ← |
tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 530,077 | 0 | T | C | 15.4%
| 126.2
/ 16.7
| 52 | Y125C (TAT→TGT) | ybbB | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (25/19); new base C (5/3); total (30/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 |
AATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTG > W3110S.gb/530010‑530147
|
aaTCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCATGAATCGCGGTCTGGCGCAGTGCCTtat > 1:4360056/1‑68 (MQ=255)
gCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCt < 1:4877436/71‑1 (MQ=255)
gCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCt < 1:168656/71‑1 (MQ=255)
cACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCtt > 1:1529282/1‑71 (MQ=255)
tCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTccac > 1:4785151/1‑70 (MQ=255)
ggTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCTCAGTGCCTTATAACCGCCTTCCACCAg > 1:821643/1‑71 (MQ=255)
ggTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAg < 1:1485498/71‑1 (MQ=255)
ggTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAg > 1:1245429/1‑71 (MQ=255)
ccAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:2769546/71‑1 (MQ=255)
ccAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:1708128/71‑1 (MQ=255)
ccAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTACACCAGCGGATaatc < 1:2578044/70‑1 (MQ=255)
aGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaatcaat < 1:4340643/71‑1 (MQ=255)
aGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaatcaat < 1:4334781/71‑1 (MQ=255)
taaaTAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCACCTTCCACCAGCGGATAATCAATccc > 1:369493/3‑71 (MQ=255)
tCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATccc > 1:615316/1‑71 (MQ=255)
cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatcaa > 1:2125759/1‑66 (MQ=255)
cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatcaa > 1:4273866/1‑66 (MQ=255)
cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatcaa > 1:165282/1‑66 (MQ=255)
cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTAATAACCGCCTTCCACCAGCGGATAATCAATcccc > 1:4953116/1‑71 (MQ=255)
cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTAATAACCGCCTTCCACCAGCGGATAATCAATcccc > 1:2181231/1‑71 (MQ=255)
cAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTAATAACCGCCTTCCACCAGCGGATAATCAATccc > 1:3086884/1‑70 (MQ=255)
aaTAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCg < 1:3630935/71‑1 (MQ=255)
tAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCg > 1:3200569/1‑69 (MQ=255)
tAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCg > 1:2168653/1‑69 (MQ=255)
aGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaa > 1:285343/1‑58 (MQ=255)
aGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaa > 1:3773060/1‑58 (MQ=255)
gCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATAATCAATcccc > 1:1442116/1‑63 (MQ=255)
cTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:4278371/55‑1 (MQ=255)
cTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCtgcatgca < 1:2130751/71‑1 (MQ=255)
cTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATAATCAATCCCCGCtgcatgca < 1:4604425/71‑1 (MQ=255)
gAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGc > 1:2026557/1‑44 (MQ=255)
gAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCg > 1:585776/1‑45 (MQ=255)
gAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaa > 1:5035042/1‑50 (MQ=255)
gAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaa > 1:248353/1‑50 (MQ=255)
gAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaa > 1:2709256/1‑50 (MQ=255)
gAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAAc > 1:207819/1‑71 (MQ=255)
aTCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:1881911/51‑1 (MQ=255)
aTCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca < 1:360941/51‑1 (MQ=255)
cgcgGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatcaat > 1:244613/1‑51 (MQ=255)
cgcgGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca > 1:2248325/1‑49 (MQ=255)
cgcgGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatca > 1:331414/1‑49 (MQ=255)
gcgcAGTGCCTTATAACCGCCTTCCACCAGCGGATaa > 1:347533/1‑37 (MQ=255)
gcgcAGTGCCTTATAACCGCCTTCCACCAGCGGATaa > 1:5068301/1‑37 (MQ=255)
gcgcAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca < 1:2592699/68‑1 (MQ=255)
gcgcAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca < 1:4449566/68‑1 (MQ=255)
cgcAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAat < 1:391659/71‑1 (MQ=255)
tGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGcac < 1:2421970/63‑1 (MQ=255)
tGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGcac < 1:4078586/63‑1 (MQ=255)
tGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATAtg < 1:712633/69‑1 (MQ=255)
tGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATAtg < 1:284474/69‑1 (MQ=255)
gCCTTATAACCGCCTTCCACCAGCGGATAATCAATCACCGCTGCATGCAACCAGCttt > 1:383452/1‑58 (MQ=255)
ccTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCtgca > 1:383236/1‑44 (MQ=255)
ttATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAg < 1:4502296/51‑1 (MQ=255)
ataACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATAtg > 1:1078189/1‑63 (MQ=255)
ataACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATAtg > 1:375011/1‑63 (MQ=255)
ataACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCt > 1:4652273/1‑71 (MQ=255)
taACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCt > 1:3938337/1‑70 (MQ=255)
taACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTg < 1:1024450/71‑1 (MQ=255)
taACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTg < 1:935175/71‑1 (MQ=255)
|
AATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTG > W3110S.gb/530010‑530147
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
