Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,238,432 |
T→C |
32.8% |
L356L (TTA→CTA) |
yeiT → |
predicted oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,238,432 | 0 | T | C | 32.8%
| 64.3
/ 53.4
| 58 | L356L (TTA→CTA) | yeiT | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (24/15); new base C (13/6); total (37/21) |
| Fisher's exact test for biased strand distribution p-value = 7.73e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 |
ACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTT > W3110S.gb/2238366‑2238494
|
aCGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTa > 1:2064993/1‑69 (MQ=255)
ggcAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGc > 1:526465/1‑71 (MQ=255)
aTAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGt < 1:2958055/68‑1 (MQ=255)
aTAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGt < 1:2756362/68‑1 (MQ=255)
aTAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGc < 1:2024427/66‑1 (MQ=255)
tAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAaca > 1:2540462/1‑71 (MQ=255)
tAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAaca > 1:4059239/1‑71 (MQ=255)
aaaaTTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAacac > 1:3902029/1‑71 (MQ=255)
attattCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTaaaa > 1:4341752/1‑65 (MQ=255)
attattCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCAt > 1:3198198/1‑71 (MQ=255)
ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa < 1:1915178/70‑1 (MQ=255)
ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa < 1:2554426/70‑1 (MQ=255)
ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa < 1:2175833/70‑1 (MQ=255)
ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa < 1:4946461/70‑1 (MQ=255)
ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCaa < 1:1520876/70‑1 (MQ=255)
gCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAacac > 1:3946936/1‑70 (MQ=255)
ccGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa < 1:2186634/71‑1 (MQ=255)
ccGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACaa < 1:3757812/71‑1 (MQ=255)
ccGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACaa < 1:3751611/71‑1 (MQ=255)
tCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGtt < 1:992404/37‑1 (MQ=255)
tCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGtt < 1:4701033/37‑1 (MQ=255)
ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa > 1:4761170/1‑68 (MQ=255)
ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa > 1:3698899/1‑68 (MQ=255)
tCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa < 1:15340/64‑1 (MQ=255)
tCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa < 1:3615097/64‑1 (MQ=255)
cATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgac > 1:1213285/1‑71 (MQ=255)
aTGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacc < 1:512319/71‑1 (MQ=255)
tGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa < 1:2129479/51‑1 (MQ=255)
tGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacc > 1:2840664/1‑70 (MQ=255)
ccAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa > 1:3695136/1‑56 (MQ=255)
aGACTAGATGCCTTTGCGTAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcga > 1:875665/1‑71 (MQ=255)
aGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcga > 1:474733/1‑71 (MQ=255)
aGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcga > 1:4603075/1‑71 (MQ=255)
tAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcgacccg > 1:3595992/1‑71 (MQ=255)
gATGCCTTTGCGGTGCTAGAGCCGCAGCGTAACACCATCAAAAcaca > 1:1751139/1‑47 (MQ=39)
gATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcgacccgc > 1:2671369/1‑70 (MQ=255)
gATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca > 1:442798/1‑47 (MQ=255)
gATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca > 1:4187449/1‑47 (MQ=255)
gATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTa > 1:1872444/1‑53 (MQ=255)
aTGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaa < 1:4626494/47‑1 (MQ=255)
aTGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgaccc < 1:4041644/60‑1 (MQ=255)
tGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc < 1:387710/52‑1 (MQ=255)
gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa > 1:2081545/1‑47 (MQ=255)
gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaa > 1:2823976/1‑46 (MQ=255)
gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCaa > 1:4709533/1‑69 (MQ=255)
gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCaa > 1:1699240/1‑69 (MQ=255)
gCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAg > 1:612582/1‑70 (MQ=255)
tttGCGGAGTTAGAGCCGCAGCGTAACACCATCaaaa < 1:2732283/37‑1 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa > 1:584410/1‑42 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa > 1:66035/1‑42 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc > 1:1776195/1‑46 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc > 1:4838791/1‑46 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc > 1:3045361/1‑46 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc > 1:409482/1‑46 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgac > 1:3925040/1‑51 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAga > 1:856933/1‑50 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCttt > 1:3250628/1‑70 (MQ=255)
tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCttt > 1:16653/1‑70 (MQ=255)
tGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCttt < 1:1486373/70‑1 (MQ=255)
tGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCtt < 1:1863101/69‑1 (MQ=255)
|
ACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTT > W3110S.gb/2238366‑2238494
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
