Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,338,549 |
G→A |
39.7% |
F185F (TTC→TTT) |
yrbE ← |
predicted toluene transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,338,549 | 0 | G | A | 39.7%
| 48.1
/ 71.2
| 63 | F185F (TTC→TTT) | yrbE | predicted toluene transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/23); new base A (12/13); total (27/36) |
| Fisher's exact test for biased strand distribution p-value = 6.05e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 |
CACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGC > W3110S.gb/3338483‑3338614
|
cacGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCagaaga < 1:2825965/71‑1 (MQ=255)
cacGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCagaaga < 1:746500/71‑1 (MQ=255)
tAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTa > 1:4103967/1‑71 (MQ=255)
aTCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:945020/71‑1 (MQ=255)
aTCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:3368489/71‑1 (MQ=255)
aTCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc < 1:220440/71‑1 (MQ=255)
tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc > 1:3703701/1‑70 (MQ=255)
gACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAAt > 1:2144966/1‑70 (MQ=255)
gTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:132552/67‑1 (MQ=255)
gTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:64762/67‑1 (MQ=255)
gTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCtt < 1:849669/71‑1 (MQ=255)
tGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTc > 1:2375849/1‑71 (MQ=255)
tGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTc > 1:4611001/1‑71 (MQ=255)
aCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCagaaga < 1:2135226/49‑1 (MQ=255)
aCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTcc < 1:5105322/70‑1 (MQ=255)
cAGATCCATACGCCAGTCGACGGCATTATGCATTGCCGACCAGAAGAACCCGCTATCAATGACTTTCCAAc > 1:2210561/1‑71 (MQ=255)
aGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACt < 1:1676664/71‑1 (MQ=255)
aGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACt < 1:1237081/71‑1 (MQ=255)
aTCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:4060064/54‑1 (MQ=255)
cGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc < 1:3161327/71‑1 (MQ=255)
cGCCAGTCGACGGAATTTTGCATTGCCGACCAgaagaa > 1:2942618/1‑38 (MQ=255)
gTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg < 1:4721554/64‑1 (MQ=255)
gTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg < 1:4108227/64‑1 (MQ=255)
gTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCagag < 1:3575149/71‑1 (MQ=255)
gTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCagag < 1:3855766/71‑1 (MQ=255)
gTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTa < 1:140067/40‑1 (MQ=255)
gTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTa < 1:1274783/40‑1 (MQ=255)
gTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCagag < 1:4293830/71‑1 (MQ=255)
gTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCa > 1:921851/1‑68 (MQ=255)
tCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:1953425/41‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCa > 1:4166506/1‑66 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCa > 1:1600952/1‑66 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc > 1:691368/1‑40 (MQ=255)
gACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGAt < 1:720035/70‑1 (MQ=255)
gACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGAt < 1:3293002/70‑1 (MQ=255)
gACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCaa > 1:4272968/1‑41 (MQ=255)
aTTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGcccc < 1:2124341/71‑1 (MQ=255)
aTTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGccc > 1:4675150/1‑70 (MQ=255)
ttttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCtt < 1:1091479/40‑1 (MQ=255)
ttttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTcc < 1:704280/43‑1 (MQ=255)
ttttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc < 1:3364743/56‑1 (MQ=255)
ttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGa > 1:4063127/1‑47 (MQ=255)
ttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGa > 1:4522884/1‑53 (MQ=255)
ttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAACAGAGATCCGccc > 1:2852105/1‑67 (MQ=255)
ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATc > 1:2272777/1‑62 (MQ=255)
ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGccc > 1:3182645/1‑67 (MQ=255)
ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAg < 1:2059950/70‑1 (MQ=255)
ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAg < 1:4362911/70‑1 (MQ=255)
tGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAAc > 1:2589010/1‑43 (MQ=255)
cATTGCCGACCAGAAGAACCCGCAATCAATGCCTTTcc < 1:4074650/38‑1 (MQ=255)
ttGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACt < 1:4132208/40‑1 (MQ=255)
tGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa > 1:1202933/1‑71 (MQ=255)
gCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGa > 1:3627464/1‑40 (MQ=255)
gCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa > 1:2921078/1‑70 (MQ=255)
gCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa < 1:1534748/70‑1 (MQ=255)
gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCg > 1:3556412/1‑57 (MQ=255)
gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa > 1:2858444/1‑70 (MQ=255)
gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCAc > 1:4812578/1‑71 (MQ=255)
gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCAc > 1:2504475/1‑71 (MQ=255)
ccGACCAGAAGAA‑‑CGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGc < 1:4992537/71‑1 (MQ=255)
gACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGc < 1:4379445/71‑1 (MQ=255)
gACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGc < 1:3818279/71‑1 (MQ=255)
gACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGc < 1:4838064/71‑1 (MQ=255)
aCCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAgaga < 1:3589974/49‑1 (MQ=255)
ccAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCag > 1:3632036/1‑45 (MQ=255)
ccAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCag > 1:134838/1‑45 (MQ=255)
ccAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAgaga < 1:2001483/48‑1 (MQ=255)
agaagaACCCGCTATCAATGCCTTTCCAACTGACGcc < 1:4276481/37‑1 (MQ=255)
|
CACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGC > W3110S.gb/3338483‑3338614
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
