Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,338,549 |
G→A |
F185F (TTC→TTT) |
yrbE ← |
predicted toluene transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,338,549 | 0 | G | A | 100.0%
| 94.1
/ NA
| 29 | F185F (TTC→TTT) | yrbE | predicted toluene transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/18); total (11/18) |
TCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCC > W3110S.gb/3338494‑3338607
|
tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa < 1:4453563/61‑1 (MQ=255)
tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa < 1:4342908/61‑1 (MQ=255)
tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa < 1:424567/61‑1 (MQ=255)
tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa < 1:2061142/61‑1 (MQ=255)
tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa < 1:2919246/61‑1 (MQ=255)
cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa < 1:622806/60‑1 (MQ=255)
cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa < 1:594991/60‑1 (MQ=255)
cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAaaagaa < 1:2956066/60‑1 (MQ=255)
cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc < 1:869837/62‑1 (MQ=255)
cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc < 1:4083907/62‑1 (MQ=255)
cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc < 1:3563311/62‑1 (MQ=255)
cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc < 1:1417805/62‑1 (MQ=255)
cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc < 1:2075617/62‑1 (MQ=255)
cAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc < 1:1931754/62‑1 (MQ=255)
cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:216669/61‑1 (MQ=255)
cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:2374078/61‑1 (MQ=255)
cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:1681677/61‑1 (MQ=255)
cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:2108541/61‑1 (MQ=255)
cAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:2709797/61‑1 (MQ=255)
aTCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCtt < 1:3604066/62‑1 (MQ=255)
ccATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc < 1:587473/45‑1 (MQ=255)
ccATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAAcc < 1:243499/45‑1 (MQ=255)
ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc > 1:1216932/1‑45 (MQ=255)
ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc > 1:1223638/1‑45 (MQ=255)
ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc > 1:1851807/1‑45 (MQ=255)
ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc > 1:1249173/1‑45 (MQ=255)
ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc > 1:3815121/1‑45 (MQ=255)
ccAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc > 1:3941619/1‑45 (MQ=255)
cAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:1133678/48‑1 (MQ=255)
gCATTTTGCATTGCCGACCAAAAGCACCCGCTATCAATGCCTTTCCAACTGCCGCCGAc > 1:3731011/1‑59 (MQ=255)
gCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc > 1:2642192/1‑59 (MQ=255)
gCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc > 1:3414740/1‑59 (MQ=255)
gCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc > 1:3356169/1‑59 (MQ=255)
ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGa < 1:1582220/53‑1 (MQ=255)
ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGa < 1:301383/53‑1 (MQ=255)
gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGc > 1:39004/1‑58 (MQ=255)
ccAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATcc < 1:2439438/62‑1 (MQ=255)
|
TCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCC > W3110S.gb/3338494‑3338607
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
