Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,657,262 |
C→T |
25.9% |
E273E (GAG→GAA) |
wzyE ← |
predicted Wzy protein involved in ECA polysaccharide chain elongation |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,657,262 | 0 | C | T | 25.9%
| 156.7
/ 56.1
| 86 | E273E (GAG→GAA) | wzyE | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Reads supporting (aligned to +/- strand): ref base C (35/28); new base T (12/10); total (48/38) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCGGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTCACATT > W3110S.gb/3657195‑3657330
|
cgcgGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGAtcctcc > 1:4237296/1‑71 (MQ=255)
gcgGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTccc < 1:4963938/71‑1 (MQ=255)
cAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGc < 1:326196/71‑1 (MQ=255)
aaTTGGAGCCAGGCCCTGTAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGc > 1:819244/1‑70 (MQ=255)
aaTTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGc > 1:559137/1‑70 (MQ=255)
aaTTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGc > 1:700431/1‑70 (MQ=255)
aTTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCga < 1:1448995/71‑1 (MQ=255)
ttGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCga < 1:3230834/70‑1 (MQ=255)
ggAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCgag > 1:3630011/1‑69 (MQ=255)
gAGCCAGGCCCTGGAAGTCGTTGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGaa < 1:4947025/70‑1 (MQ=255)
gAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGaa < 1:3436229/70‑1 (MQ=255)
gAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGaa < 1:4358206/70‑1 (MQ=255)
gCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGgt < 1:2789630/71‑1 (MQ=255)
ccAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGgtg < 1:2410135/71‑1 (MQ=255)
gCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCgag < 1:3483914/61‑1 (MQ=255)
cccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATtct > 1:3275543/1‑49 (MQ=255)
cccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATtct > 1:4930722/1‑49 (MQ=255)
cccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgcg < 1:5134665/71‑1 (MQ=255)
ccTTGAAGTCGATGTTGTCGTAGTTCTGCAACATCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc > 1:2229556/1‑69 (MQ=255)
ccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc > 1:4116187/1‑69 (MQ=255)
ccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc > 1:4820346/1‑69 (MQ=255)
ccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACAGCGAGAAGGTGTcgc > 1:1196049/1‑69 (MQ=255)
gAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGt < 1:1570024/69‑1 (MQ=255)
aaGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCa > 1:505399/1‑47 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACgg > 1:205035/1‑48 (MQ=255)
gTCGATGTTGTCGTAGCTCTGCAACAGCAACGCCAGATTCTCCCACgg > 1:2459675/1‑48 (MQ=255)
cGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTgaga > 1:4840137/1‑68 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:3969625/1‑69 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:520406/1‑69 (MQ=255)
tgttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATagag > 1:2713406/1‑70 (MQ=255)
gttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAgaga > 1:2431832/1‑70 (MQ=255)
gttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAgaga > 1:4656409/1‑70 (MQ=255)
ttgtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGaa > 1:751446/1‑47 (MQ=255)
ttgtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAg < 1:3925429/48‑1 (MQ=255)
ttgtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGAt > 1:349385/1‑64 (MQ=255)
ttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaa < 1:4060153/70‑1 (MQ=255)
ttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaa < 1:857133/70‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg > 1:5842/1‑71 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAg < 1:407108/46‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAg < 1:1533089/46‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg > 1:1642710/1‑71 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg > 1:4491743/1‑71 (MQ=255)
tCGTAGCTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg < 1:971212/70‑1 (MQ=255)
gTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAg > 1:1455125/1‑53 (MQ=255)
gTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAg > 1:1942499/1‑53 (MQ=255)
tAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAg < 1:2718357/52‑1 (MQ=255)
tAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAg < 1:1585836/52‑1 (MQ=255)
gTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAg > 1:215276/1‑40 (MQ=255)
ttCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc < 1:2399736/46‑1 (MQ=255)
cTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:594085/1‑71 (MQ=255)
cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaa > 1:3589396/1‑59 (MQ=255)
cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:1133549/1‑71 (MQ=255)
cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:594067/1‑71 (MQ=255)
cTGCAACAGCAACGCCAGATTCTCCCACGGAGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:3727403/1‑71 (MQ=255)
cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg < 1:4854949/68‑1 (MQ=255)
cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg < 1:2511174/68‑1 (MQ=255)
cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACATATAGAACGCt < 1:4777252/70‑1 (MQ=255)
aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:3631769/1‑50 (MQ=255)
aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:2471381/1‑50 (MQ=255)
aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:453331/1‑50 (MQ=255)
aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCtt < 1:1294256/70‑1 (MQ=255)
aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTc < 1:4832375/71‑1 (MQ=255)
aCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg < 1:2535641/66‑1 (MQ=255)
aCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCa < 1:2080751/71‑1 (MQ=255)
cAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCAt > 1:1524116/1‑71 (MQ=255)
cAGAAACGCCAGATTATCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTc > 1:1970497/1‑69 (MQ=255)
aGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:3385674/1‑39 (MQ=255)
aGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATc > 1:2606186/1‑71 (MQ=255)
gCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTgaga > 1:3541634/1‑44 (MQ=255)
gCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTgaga > 1:2038536/1‑44 (MQ=255)
gCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcg > 1:3381764/1‑71 (MQ=255)
gCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATc > 1:2168600/1‑70 (MQ=255)
aCGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgcag > 1:4806466/1‑69 (MQ=255)
gCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgccctc < 1:4529637/71‑4 (MQ=255)
gCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCt > 1:3890424/1‑71 (MQ=255)
ccAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTc < 1:4431622/71‑1 (MQ=255)
ccAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTc < 1:2797209/71‑1 (MQ=255)
ccAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTgaga > 1:3286395/1‑38 (MQ=255)
ccAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg < 1:2232831/48‑1 (MQ=255)
cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgc > 1:1580324/1‑65 (MQ=255)
cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTca > 1:1760305/1‑71 (MQ=255)
cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTca < 1:1484149/71‑1 (MQ=255)
cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTc < 1:2256105/70‑1 (MQ=255)
aGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTca > 1:256936/1‑70 (MQ=255)
gATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTcaca < 1:3865546/71‑1 (MQ=255)
gATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTcaca < 1:2396312/71‑1 (MQ=255)
aTTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTTTAGAACGCTTCATCGCCGCTcaca < 1:1929552/70‑1 (MQ=255)
aTTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAg < 1:2754788/49‑1 (MQ=255)
ttCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTCAcatt > 1:4259273/1‑71 (MQ=255)
tctcCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgc < 1:3446915/60‑1 (MQ=255)
tctcCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgc < 1:3339340/60‑1 (MQ=255)
|
CGCGGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTCACATT > W3110S.gb/3657195‑3657330
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
