Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,657,262 |
C→T |
E273E (GAG→GAA) |
wzyE ← |
predicted Wzy protein involved in ECA polysaccharide chain elongation |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,657,262 | 0 | C | T | 100.0%
| 222.7
/ NA
| 68 | E273E (GAG→GAA) | wzyE | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/40); total (28/40) |
GGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCAT > W3110S.gb/3657206‑3657317
|
ggAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:3450039/62‑1 (MQ=255)
ggAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:3365309/62‑1 (MQ=255)
ggAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:3897219/62‑1 (MQ=255)
ggAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:1452357/62‑1 (MQ=255)
ggAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:3831097/62‑1 (MQ=255)
ccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAg < 1:2841264/62‑1 (MQ=255)
aGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcg < 1:3529301/62‑1 (MQ=255)
aGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcg < 1:4673551/62‑1 (MQ=255)
aGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcg < 1:4126270/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:2667166/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:4076459/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:2683760/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:4078619/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:215198/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:2144019/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:3206875/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:4468886/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:3329206/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:1424155/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:527719/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:705577/62‑1 (MQ=255)
gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:1059469/62‑1 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTcc > 1:3011469/1‑40 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:30497/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:2856212/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:2671468/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:2275897/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:3968705/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:2545909/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:2212333/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:1879140/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:1866253/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:1714106/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:1451553/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:1444572/1‑61 (MQ=255)
gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCAACGGCGAGAAGGTGTcgc > 1:2208420/1‑59 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:103577/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:3885131/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:4284600/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:3764026/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:903742/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:963899/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:3442539/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:3335897/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:1616050/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:2434524/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCa < 1:2572802/36‑1 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTga > 1:1025130/1‑59 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTga > 1:110754/1‑59 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTga > 1:1259731/1‑59 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTga > 1:1952420/1‑59 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTga > 1:2908780/1‑59 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTga > 1:3842076/1‑59 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTga > 1:3620716/1‑59 (MQ=255)
gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTga > 1:378799/1‑59 (MQ=255)
gTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATag < 1:361088/61‑1 (MQ=255)
gTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATag < 1:3159935/61‑1 (MQ=255)
gTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATag < 1:669578/61‑1 (MQ=255)
gTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATag < 1:1667604/61‑1 (MQ=255)
gTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATag < 1:560662/61‑1 (MQ=255)
gTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATag < 1:1372753/61‑1 (MQ=255)
aGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc < 1:3904364/48‑1 (MQ=255)
aaCAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGAt > 1:1533107/1‑49 (MQ=255)
aaCAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGAt > 1:4643124/1‑49 (MQ=255)
aaCAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGAt > 1:4143566/1‑49 (MQ=255)
aaCAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGAt > 1:3279476/1‑49 (MQ=255)
cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCAt > 1:2589245/1‑62 (MQ=255)
cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCAt > 1:3568069/1‑62 (MQ=255)
|
GGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCAT > W3110S.gb/3657206‑3657317
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
