Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R2
|
159 |
90.8 |
4763082 |
92.7% |
4415377 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
406,585 |
A→G |
12.3% |
D8D (GAT→GAC) |
ybeB ← |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 406,585 | 0 | A | G | 12.3%
| 226.1
/ 20.4
| 81 | D8D (GAT→GAC) | ybeB | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (27/44); new base G (2/8); total (29/52) |
| Fisher's exact test for biased strand distribution p-value = 3.18e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 |
TGGATTTGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATAAAAATGGTCTGT > minE/406515‑406649
|
tGGATTTGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACaaaa > 1:4635296/1‑71 (MQ=255)
gATTTGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAAt < 1:4569195/70‑1 (MQ=255)
gATTTGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAAt < 1:1402359/70‑1 (MQ=255)
gATTTGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAAt < 1:1777813/70‑1 (MQ=255)
aTTTGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATcc < 1:1635496/71‑1 (MQ=255)
aTTTGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATcc < 1:1640913/71‑1 (MQ=255)
tttGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCt > 1:1003388/1‑71 (MQ=255)
tttGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCt > 1:2179302/1‑71 (MQ=255)
gCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTgg < 1:3741250/70‑1 (MQ=255)
cccTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAg < 1:4684378/71‑1 (MQ=255)
cccTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAg < 1:1362087/71‑1 (MQ=255)
cccTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAg < 1:2532706/71‑1 (MQ=255)
cTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTg < 1:3676372/71‑1 (MQ=255)
cTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTg > 1:3303855/1‑71 (MQ=255)
aaCGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCGTCAATTTTGTCGATAACAAAGTCCTGGAGTGCtt < 1:4221199/71‑1 (MQ=255)
aaCGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAGTCCTg < 1:2981549/63‑1 (MQ=255)
aaCGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAGTCCTg < 1:1569433/63‑1 (MQ=255)
aaCGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAGTCCTGGAGTGCtt < 1:4702282/71‑1 (MQ=255)
aaCGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAGTCCTGGAGTGCtt < 1:1218317/71‑1 (MQ=255)
aaCGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAGTCCTGGAGTGCtt < 1:1267448/71‑1 (MQ=255)
aaCGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGc < 1:828900/69‑1 (MQ=255)
cGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAAt > 1:189966/1‑57 (MQ=255)
cGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAAt > 1:807541/1‑57 (MQ=255)
cGTCTAAGGCGATGATGTCCTGACCTTTAAGGTCATCAATTTTGTCGATAACAAAAt > 1:1794421/1‑57 (MQ=255)
tCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCt < 1:2838573/58‑1 (MQ=255)
tAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGa > 1:3031482/1‑59 (MQ=255)
aGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGc < 1:3510138/71‑1 (MQ=255)
aGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGc < 1:2611574/71‑1 (MQ=255)
aGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGc < 1:2168353/71‑1 (MQ=255)
aGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGc < 1:2858627/71‑1 (MQ=255)
ggCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCa < 1:78348/71‑1 (MQ=255)
gCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTa > 1:4714632/1‑63 (MQ=255)
gCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTa > 1:4670722/1‑63 (MQ=255)
cGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAg < 1:4133886/71‑1 (MQ=255)
gatgTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTAcc > 1:4190851/1‑60 (MQ=255)
gatgTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTAcc > 1:4736644/1‑60 (MQ=255)
gatgTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGtttt > 1:2251629/1‑71 (MQ=255)
gatgTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGtttt > 1:3297455/1‑71 (MQ=255)
gTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTccc > 1:3942917/1‑71 (MQ=255)
gTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTccc > 1:1679103/1‑71 (MQ=255)
ccTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATcc > 1:1950462/1‑41 (MQ=255)
cTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCtt < 1:1377126/50‑1 (MQ=255)
cTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCt < 1:3280898/71‑1 (MQ=255)
cTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCt < 1:1463963/71‑1 (MQ=255)
gcccTTTGAGGTGATCAATTTTTTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCAgg > 1:492041/3‑71 (MQ=255)
gACCTTTGAGGTCATCAATTTTGTCGATAACAAAATACTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTgg > 1:588068/1‑71 (MQ=255)
aCCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTggg > 1:2499772/1‑71 (MQ=255)
tttGAGGTCATCAATTTTGTCGATAACAAAATCCt < 1:3272249/35‑1 (MQ=255)
tttGAGGTCATCAATTTTGTCGATAACAAAATCCt < 1:498776/35‑1 (MQ=255)
tttGAGGTCATCAATTTTGTCGATAACAAAATCCt < 1:793369/35‑1 (MQ=255)
ttGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGc < 1:1643249/51‑1 (MQ=255)
tGAGGTCATCAATTTTGTCGATAACAAAGTCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTggg > 1:1489510/1‑66 (MQ=255)
gAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGa < 1:3142444/68‑1 (MQ=255)
aGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCa < 1:4283915/71‑1 (MQ=255)
aGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCa < 1:4525109/71‑1 (MQ=255)
aGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCa < 1:2097213/71‑1 (MQ=255)
catcaATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGt > 1:4582131/1‑61 (MQ=255)
catcaATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGt > 1:2450093/1‑61 (MQ=255)
catcaATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCaaa > 1:2179370/1‑69 (MQ=255)
atcaATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGt < 1:3361062/60‑1 (MQ=255)
atcaATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGt < 1:3959961/60‑1 (MQ=255)
atcaATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGt < 1:2964374/60‑1 (MQ=255)
caATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGt < 1:1817425/58‑1 (MQ=255)
caATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGa > 1:4405579/1‑60 (MQ=255)
caATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGa > 1:4113722/1‑70 (MQ=255)
aaTTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGAt > 1:3595887/1‑70 (MQ=255)
aaTTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGAt > 1:3544434/1‑70 (MQ=255)
aaTTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGAt > 1:3382374/1‑70 (MQ=255)
ttttGTCGATAACAAAATCCTGGAGTGCTTTACCCt < 1:3389442/36‑1 (MQ=255)
ttttGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATaaa < 1:3929604/71‑1 (MQ=255)
tCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATAAAAATg < 1:1257417/70‑1 (MQ=255)
cGATAACAAAATCCTGGAGTGCTTTACCCTGCAAg < 1:1889415/35‑1 (MQ=255)
taTAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTg < 1:1184553/44‑1 (MQ=255)
gATAACAAAATCCTGGAGTGCTTTACCCTGCAAGtttt < 1:559033/38‑1 (MQ=255)
gATAACAAAATCCTGGAGTGCTTTACCCTGCAAGtttt < 1:2326202/38‑1 (MQ=255)
gATAACAAAATCCTGGAGTGCTTTACCCTGCAAGtttt < 1:932513/38‑1 (MQ=255)
gATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATAAAAATg < 1:916325/68‑1 (MQ=255)
gATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATAAAAATGGt < 1:2174579/70‑1 (MQ=255)
tAACAAAGTCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATAAAAATGGTCTg < 1:4476063/71‑1 (MQ=255)
tAACAAAGTCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATAAAAATGGTCTg > 1:4198354/1‑71 (MQ=255)
tAACAAAGTCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATAAAAATGGTCTg < 1:2874483/71‑1 (MQ=255)
aCAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATAAAAATGGTCTGt < 1:4251557/70‑1 (MQ=255)
aaaTCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGaa > 1:3967522/1‑44 (MQ=255)
aaaTCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGaa > 1:3767806/1‑44 (MQ=255)
aaaTCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGaa > 1:3001388/1‑44 (MQ=255)
|
TGGATTTGCCCTGAACGTCTAAGGCGATGATGTCCTGACCTTTGAGGTCATCAATTTTGTCGATAACAAAATCCTGGAGTGCTTTACCCTGCAAGTTTTCCCCCTGGGTGAATCAAATAGATAAAAATGGTCTGT > minE/406515‑406649
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
