Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R2
|
159 |
90.8 |
4763082 |
92.7% |
4415377 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
965,646 |
C→T |
8.9% |
G83D (GGC→GAC) |
ydeM ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 965,646 | 0 | C | T | 8.9%
| 315.0
/ 14.5
| 101 | G83D (GGC→GAC) | ydeM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (39/53); new base T (3/6); total (42/59) |
| Fisher's exact test for biased strand distribution p-value = 7.32e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 101 total aligned reads displayed.
AAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCACC > minE/965579‑965713
|
aaaGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTg < 1:3624547/71‑1 (MQ=255)
aGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCa > 1:3797910/1‑71 (MQ=255)
aGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCa > 1:416174/1‑71 (MQ=255)
ggCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt < 1:4535664/71‑1 (MQ=255)
gCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATa < 1:313503/71‑1 (MQ=255)
cacaCCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg < 1:3236791/71‑1 (MQ=255)
acacCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGc < 1:2191605/66‑1 (MQ=255)
acacCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGc < 1:3138502/71‑1 (MQ=255)
acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCa > 1:3142641/1‑65 (MQ=255)
acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:4284484/1‑66 (MQ=255)
acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:2224367/1‑66 (MQ=255)
acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgt < 1:3654183/71‑1 (MQ=255)
acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgt < 1:3407338/71‑1 (MQ=255)
ccATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgtt > 1:1158337/1‑71 (MQ=255)
cattcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttg < 1:2282/71‑1 (MQ=255)
ttcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg < 1:3437677/64‑1 (MQ=255)
ttcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg < 1:2505698/64‑1 (MQ=255)
cattATTCAATAAAATGCCATTAGTTTGTAATGCATTAAAAAAACGTTATTGTCCTGc > 1:2511746/1‑58 (MQ=38)
aaTAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGaa > 1:4339535/1‑70 (MQ=255)
aTAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTATGCATAGCGTTGTTGATAGTGAAt < 1:3310844/70‑1 (MQ=255)
aTAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttg > 1:278473/1‑58 (MQ=255)
aTAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttg > 1:4703402/1‑58 (MQ=255)
tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTg < 1:3453377/47‑1 (MQ=255)
tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTg < 1:4093703/47‑1 (MQ=255)
tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTg < 1:218818/47‑1 (MQ=255)
tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATaa > 1:2650890/1‑71 (MQ=255)
aaaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGACAGTGAATAAc > 1:2320346/1‑71 (MQ=255)
aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:3173023/1‑48 (MQ=255)
aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:4130173/1‑48 (MQ=255)
aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:3934992/1‑48 (MQ=255)
aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:2240723/1‑48 (MQ=255)
aaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACtt < 1:4030711/71‑1 (MQ=255)
aTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:805488/71‑1 (MQ=255)
tGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:4216762/71‑1 (MQ=255)
tGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:4043756/71‑1 (MQ=255)
cATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACgg < 1:554133/71‑1 (MQ=255)
ttCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg < 1:1553363/42‑1 (MQ=255)
ttCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGc < 1:304187/43‑1 (MQ=255)
tCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATa < 1:3742102/52‑1 (MQ=255)
tCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATa < 1:3100820/52‑1 (MQ=255)
cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCg > 1:3032696/1‑42 (MQ=255)
cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCg > 1:4279856/1‑42 (MQ=255)
cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGttgttg > 1:2898406/1‑48 (MQ=255)
cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaa > 1:1756840/1‑70 (MQ=255)
cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaa > 1:3559710/1‑70 (MQ=255)
tttGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaaa < 1:2630350/71‑1 (MQ=255)
tttGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaaa < 1:346300/71‑1 (MQ=255)
tttGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaaa < 1:3818530/71‑1 (MQ=255)
tGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAAt < 1:3122803/70‑1 (MQ=255)
gTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATc < 1:4757292/70‑1 (MQ=255)
tAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATcc < 1:2754532/70‑1 (MQ=255)
tAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATcc < 1:3498319/70‑1 (MQ=255)
aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:1102307/64‑1 (MQ=255)
aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:4053097/64‑1 (MQ=255)
aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:2013593/64‑1 (MQ=255)
aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:1878530/64‑1 (MQ=255)
aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:1660388/64‑1 (MQ=255)
aTGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAgg > 1:4387277/1‑71 (MQ=255)
aTGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAgg > 1:1629943/1‑71 (MQ=255)
tGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc < 1:3468674/71‑1 (MQ=255)
tGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc < 1:2359000/71‑1 (MQ=255)
tGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc < 1:1485514/71‑1 (MQ=255)
tGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc < 1:374060/71‑1 (MQ=255)
cATTAAAAATACGTTTTTGGCCTGCATAGCGttgttg < 1:3160746/37‑1 (MQ=255)
cATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc > 1:583509/1‑69 (MQ=255)
ttAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTg < 1:3823301/41‑1 (MQ=255)
tAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:838688/1‑70 (MQ=255)
aaaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaaa > 1:2860158/1‑58 (MQ=255)
aaaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:3191647/1‑69 (MQ=255)
aaaaaTACGTTTTTGGCCTGCACAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:2322061/1‑69 (MQ=255)
aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATa > 1:922253/1‑35 (MQ=255)
aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATa > 1:3236658/1‑35 (MQ=255)
aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATa > 1:3231282/1‑35 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagcca < 1:630486/70‑1 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagcca < 1:2537879/70‑1 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:108122/1‑68 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:3627041/1‑68 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:3621812/1‑68 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:1313779/1‑68 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:151067/1‑68 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:4628995/1‑68 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGccagccag < 1:3740763/71‑1 (MQ=255)
aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGccagccag < 1:3068130/71‑1 (MQ=255)
aaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCagag > 1:3216239/1‑71 (MQ=255)
aTACGTTTTTGGCCTGCATAACGTTGTTGATAGTGAATAACTTTACGGaaaaaa < 1:768553/54‑1 (MQ=255)
tACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTg < 1:2740340/71‑1 (MQ=255)
aCGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTgg > 1:327260/1‑71 (MQ=255)
cGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTggg < 1:4679781/71‑1 (MQ=255)
gTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGt > 1:3825159/1‑71 (MQ=255)
gTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGt < 1:4187411/71‑1 (MQ=255)
tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:2681045/39‑1 (MQ=255)
tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:1232985/39‑1 (MQ=255)
tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:1253689/39‑1 (MQ=255)
tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:688739/39‑1 (MQ=255)
tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:3177231/39‑1 (MQ=255)
tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGtt > 1:4695323/1‑71 (MQ=255)
tttttGGCCTGCATAGAGTTGTTGATAGTGAATAACttt < 1:1641644/39‑1 (MQ=255)
tttGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAg > 1:1692874/1‑52 (MQ=255)
ggCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCAcc < 1:2425779/70‑1 (MQ=255)
gCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaa < 1:4726686/40‑1 (MQ=255)
|
AAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCACC > minE/965579‑965713
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
